Restriction map for
pFN21AE1435
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BalI BamHI
BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI BmyI
BpmI Bpu1102I BpuAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BsmI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRI EcoRV FauI FbaI
FseI FspI HaeII HhaI Hin1I
HinP1I KasI KpnI Ksp632I KspI
MaeI MaeIII MamI MfeI MflI
MluI MluNI MroI MscI MspA1I
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PpuMI PshAI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tsp45I
Tth111I Van91I XbaI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AflIII 274 ACACGT A^C[GA][CT]GT
AluI 686 AGCT AG^CT
AlwI 435 GGATC GGATC(4/5)
AlwNI 596 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 219 CCTCAGG CC^T[ACGT]AGG
AseI 148 ATTAAT AT^TAAT
AsnI 148 ATTAAT AT^TAAT
AvaI 710 CCCGGG C^[CT]CG[GA]G
AvaII 394 GGACC G^G[AT]CC
BbrPI 275 CACGTG CAC^GTG
BbvI 211 GCAGC GCAGC(8/12)
BsaAI 275 CACGTG [CT]AC^GT[GA]
BsmAI 517 GAGAC GTCTC(1/5)
BsoBI 710 CCCGGG C^[CT]CG[GA]G
Bsu36I 219 CCTCAGG CC^T[ACGT]AGG
Cac8I 484 GCCTGC GC[ACGT]^[ACGT]GC
Cfr13I 394 GGACC G^G[ACGT]CC
DrdI 183 GACATCTCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 219 CCTCAGG CC^T[ACGT]AGG
EcoT14I 360 CCATGG C^C[AT][AT]GG
EcoT22I 584 ATGCAT ATGCA^T
Fnu4HI 211 GCAGC GC^[ACGT]GC
HapII 711 CCGG C^CGG
HgaI 757 GACGC GACGC(5/10)
HindIII 685 AAGCTT A^AGCTT
HpaI 715 GTTAAC GTT^AAC
HpaII 711 CCGG C^CGG
HphI 364 GGTGA GGTGA(8/7)
ItaI 211 GCAGC GC^[ACGT]GC
MaeII 276 ACGT A^CGT
MslI 174 CACTGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 711 CCGG C^CGG
MwoI 326 GCAAAAGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 360 CCATGG C^CATGG
NsiI 584 ATGCAT ATGCA^T
PmaCI 275 CACGTG CAC^GTG
PmlI 275 CACGTG CAC^GTG
PshBI 148 ATTAAT AT^TAAT
Sau96I 394 GGACC G^G[ACGT]CC
SexAI 600 ACCTGGT A^CC[AT]GGT
SmaI 710 CCCGGG CCC^GGG
StuI 482 AGGCCT AGG^CCT
StyI 360 CCATGG C^C[AT][AT]GG
TaqI 206 TCGA T^CGA
TseI 211 GCAGC G^C[AT]GC
TthHB8I 206 TCGA T^CGA
XcmI 35 CCATTAAAAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 710 CCCGGG C^CCGGG
XmaI 710 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 15 enzymes) #
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AfaI 197 GTAC GT^AC
494 GTAC
BcnI 710 CCCGG CC^[GC]GG
711 CCGGG
BsiYI 124 CCTGAGATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
720 CCGAAAGGTGG
BslI 124 CCTGAGATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
720 CCGAAAGGTGG
BstF5I 645 CATCC GGATG(2/0)
630 GGATG
DsaI 360 CCATGG C^C[GA][CT]GG
489 CCGTGG
FokI 645 CATCC GGATG(9/13)
630 GGATG
HaeIII 483 GGCC GG^CC
570 GGCC
HincII 400 GTTGAC GT[CT]^[GA]AC
715 GTTAAC
HindII 400 GTTGAC GT[CT]^[GA]AC
715 GTTAAC
NciI 710 CCCGG CC^[GC]GG
711 CCGGG
NlaIV 48 GGAACC GG[ACGT]^[ACGT]CC
634 GGCTCC
PleI 82 GACTC GAGTC(4/5)
742 GAGTC
RsaI 197 GTAC GT^AC
494 GTAC
SfaNI 208 GATGC GCATC(5/9)
297 GATGC
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# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AcsI ApoI BsaJI BsrI BssKI
BstNI DdeI DpnII EcoRII HinfI
MboI MboII MnlI MseI MvaI
NdeII NlaIII Sau3AI ScrFI TfiI
Tru9I Tsp509I TspRI