Restriction map for
pFN21AE6330
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# NO CUT ENZYMES ( 141 enzymes) #
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AatII AccIII AclI AcsI AcyI
AflII AflIII AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BcgI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BseAI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrGI BssHII
Bst1107I BstBI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoT22I FbaI FseI FspI
HaeII Hin1I HincII HindII HindIII
HpaI KasI Ksp632I KspI MamI
MfeI MflI MluI MluNI MroI
MscI MslI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TaqI TfiI Tsp509I
Tth111I TthHB8I XbaI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 766 GGTACC G^GTACC
AccI 301 GTCTAC GT^[AC][GT]AC
AgeI 113 ACCGGT A^CCGGT
Asp718I 766 GGTACC G^GTACC
AvaI 12 CCCGGG C^[CT]CG[GA]G
BciVI 292 GTATCC GTATCC(6/5)
BfaI 410 CTAG C^TAG
BsaI 343 GGTCTC GGTCTC(1/5)
BsaWI 113 ACCGGT [AT]^CCGG[AT]
BsgI 97 CTGCAC GTGCAG(16/14)
BsmBI 475 CGTCTC CGTCTC(1/5)
BsoBI 12 CCCGGG C^[CT]CG[GA]G
BsrDI 867 CATTGC GCAATG(2/0)
BsrFI 113 ACCGGT [GA]^CCGG[CT]
BssSI 947 CACGAG CACGAG(-5/-1)
BstAPI 742 GCAGTGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 24 GGTGACC G^GT[ACGT]ACC
BstPI 24 GGTGACC G^GT[ACGT]ACC
CfoI 70 GCGC GCG^C
Cfr10I 113 ACCGGT [GA]^CCGG[CT]
EaeI 958 CGGCCA [CT]^GGCC[GA]
EcoO65I 24 GGTGACC G^GT[ACGT]ACC
EcoRV 850 GATATC GAT^ATC
HgaI 711 GACGC GACGC(5/10)
HhaI 70 GCGC GCG^C
HinP1I 70 GCGC G^CGC
KpnI 766 GGTACC GGTAC^C
MaeI 410 CTAG C^TAG
MaeII 506 ACGT A^CGT
MseI 625 TTAA T^TAA
PflMI 961 CCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 113 ACCGGT A^CCGGT
PpuMI 922 GGGACCC [GA]G^G[AT]CC[CT]
PstI 740 CTGCAG CTGCA^G
SacI 897 GAGCTC GAGCT^C
SmaI 12 CCCGGG CCC^GGG
SmlI 1107 CTTGAG C^T[CT][GA]AG
StuI 979 AGGCCT AGG^CCT
Tru9I 625 TTAA T^TAA
Van91I 961 CCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1146 CCAGAGTCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 12 CCCGGG C^CCGGG
XmaI 12 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccII 69 CGCG CG^CG
80 CGCG
AlwI 243 GGATC GGATC(4/5)
561 GGATC
AlwNI 728 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1018 CAGGCCCTG
AspHI 723 GAGCAC G[AT]GC[AT]^C
897 GAGCTC
BbsI 180 GTCTTC GAAGAC(2/6)
645 GTCTTC
BpuAI 180 GTCTTC GAAGAC(2/6)
645 GTCTTC
BseRI 461 CTCCTC GAGGAG(10/8)
835 GAGGAG
BsiHKAI 723 GAGCAC G[AT]GC[AT]^C
897 GAGCTC
BsmFI 922 GGGAC GGGAC(10/14)
327 GTCCC
BstUI 69 CGCG CG^CG
80 CGCG
DpnII 244 GATC ^GATC
562 GATC
MboI 244 GATC ^GATC
562 GATC
MvnI 69 CGCG CG^CG
80 CGCG
NdeII 244 GATC ^GATC
562 GATC
PvuII 88 CAGCTG CAG^CTG
372 CAGCTG
Sau3AI 244 GATC ^GATC
562 GATC
SfaNI 862 GATGC GCATC(5/9)
422 GCATC
SfcI 369 CTACAG C^T[GA][CT]AG
740 CTGCAG
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# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AfaI AluI ApaI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaJI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DraII DsaI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeIII MboII MnlI
MspA1I MspI MvaI MwoI NciI
NcoI NlaIII NlaIV PleI RsaI
Sau96I ScrFI StyI TseI Tsp45I
TspRI