Restriction map for
pFN21AE2277
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AlwNI AocI Aor51HI ApaI
ApoI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HaeII Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MseI MslI MunI NarI NciI
NcoI NdeI NheI NlaIII NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI TfiI Tru9I Tsp509I
TspRI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AfaI 171 GTAC GT^AC
Alw44I 91 GTGCAC G^TGCAC
AlwI 186 GATCC GGATC(4/5)
ApaLI 91 GTGCAC G^TGCAC
AscI 139 GGCGCGCC GG^CGCGCC
BalI 257 TGGCCA TGG^CCA
BanII 277 GAGCTC G[GA]GC[CT]^C
BbsI 225 GAAGAC GAAGAC(2/6)
BfaI 59 CTAG C^TAG
BpuAI 225 GAAGAC GAAGAC(2/6)
BsaI 67 GGTCTC GGTCTC(1/5)
BsaJI 80 CCGTGG C^C[ACGT][ACGT]GG
BseRI 274 GAGGAG GAGGAG(10/8)
BsmAI 68 GTCTC GTCTC(1/5)
BsrBI 124 GAGCGG CCGCTC(-3/-3)
BssHII 140 GCGCGC G^CGCGC
BssKI 189 CCTGG ^CC[ACGT]GG
BstAPI 93 GCACCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 189 CCTGG CC^[AT]GG
BstYI 185 AGATCC [GA]^GATC[CT]
DpnII 186 GATC ^GATC
DsaI 80 CCGTGG C^C[GA][CT]GG
EaeI 257 TGGCCA [CT]^GGCC[GA]
EarI 54 CTCTTC CTCTTC(1/4)
EcoRII 189 CCTGG ^CC[AT]GG
HgaI 243 GCGTC GACGC(5/10)
HinfI 48 GACTC G^A[ACGT]TC
HphI 302 TCACC GGTGA(8/7)
Ksp632I 54 CTCTTC CTCTTC(1/4)
MaeI 59 CTAG C^TAG
MaeII 9 ACGT A^CGT
MaeIII 301 GTCAC ^GT[ACGT]AC
MboI 186 GATC ^GATC
MflI 185 AGATCC [GA]^GATC[CT]
MluNI 257 TGGCCA TGG^CCA
MscI 257 TGGCCA TGG^CCA
MspA1I 158 CAGCGG C[AC]G^C[GT]G
MvaI 189 CCTGG CC^[AT]GG
NaeI 136 GCCGGC GCC^GGC
NdeII 186 GATC ^GATC
NgoMIV 136 GCCGGC G^CCGGC
NlaIV 133 GGGGCC GG[ACGT]^[ACGT]CC
PleI 48 GACTC GAGTC(4/5)
RsaI 171 GTAC GT^AC
SacI 277 GAGCTC GAGCT^C
SapI 53 GCTCTTC GCTCTTC(1/4)
Sau3AI 186 GATC ^GATC
ScrFI 189 CCTGG CC^[ACGT]GG
SfaNI 233 GCATC GCATC(5/9)
SgrAI 143 CGCCGGTG C[GA]^CCGG[CT]G
Tsp45I 301 GTCAC ^GT[GC]AC
XhoII 185 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 13 enzymes) #
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AluI 278 AGCT AG^CT
287 AGCT
AspHI 91 GTGCAC G[AT]GC[AT]^C
277 GAGCTC
BbvI 157 GCAGC GCAGC(8/12)
288 GCTGC
BmyI 91 GTGCAC G[AGT]GC[ACT]^C
277 GAGCTC
BpmI 70 CTCCAG CTGGAG(16/14)
166 CTGGAG
BsiHKAI 91 GTGCAC G[AT]GC[AT]^C
277 GAGCTC
Bsp1286I 91 GTGCAC G[AGT]GC[ACT]^C
277 GAGCTC
BstF5I 237 CATCC GGATG(2/0)
261 CATCC
DdeI 38 CTAAG C^T[ACGT]AG
362 CTGAG
FokI 237 CATCC GGATG(9/13)
261 CATCC
MboII 225 GAAGA GAAGA(8/7)
55 TCTTC
MnlI 23 GAGG CCTC(7/6)
274 GAGG
TseI 157 GCAGC G^C[AT]GC
288 GCTGC
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# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AccII AciI BsiYI BslI BsrFI
BsrI BstUI Cac8I CfoI Cfr10I
Cfr13I FauI Fnu4HI HaeIII HapII
HhaI HinP1I HpaII ItaI MspI
MvnI MwoI Sau96I