Restriction map for
pFN21AE9674
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# NO CUT ENZYMES ( 92 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AflIII AgeI AocI Aor51HI ApaI
AscI AseI AsnI Asp718I AspI
AviII AvrII BamHI BciVI BfrI
BglI BlnI BsaBI BseAI BsiWI
Bsp1407I BspEI BspLU11I BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CpoI DraIII DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV FspI HindIII
HpaI KpnI KspI MamI MfeI
MluI MroI MunI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I RsrII SacII SalI SapI
SbfI ScaI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AatII 1945 GACGTC GACGT^C
AccII 2449 CGCG CG^CG
AfaI 759 GTAC GT^AC
AhdI 2793 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2452 GTGCAC G^TGCAC
AlwNI 898 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 2452 GTGCAC G^TGCAC
AspEI 2793 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 111 CTCGGG C^[CT]CG[GA]G
BalI 500 TGGCCA TGG^CCA
BbeI 1931 GGCGCC GGCGC^C
BbrPI 2245 CACGTG CAC^GTG
BbvCI 2440 GCTGAGG CCTCAGC(-5/-2)
BcgI 1523 GCATCAACGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1568 TGATCA T^GATCA
BglII 2642 AGATCT A^GATCT
BsaI 2578 GAGACC GGTCTC(1/5)
BsaWI 1424 ACCGGA [AT]^CCGG[AT]
BsgI 2248 GTGCAG GTGCAG(16/14)
BsiEI 735 CGATCG CG[GA][CT]^CG
BsmBI 2852 CGTCTC CGTCTC(1/5)
BsmI 2483 GCATTC GAATGC(1/-1)
BsoBI 111 CTCGGG C^[CT]CG[GA]G
BspDI 2263 ATCGAT AT^CGAT
BspHI 258 TCATGA T^CATGA
BspMI 893 ACCTGC ACCTGC(4/8)
BstUI 2449 CGCG CG^CG
BstXI 2471 CCAGCTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 2642 AGATCT [GA]^GATC[CT]
ClaI 2263 ATCGAT AT^CGAT
Eam1105I 2793 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 1002 ATGCAT ATGCA^T
FbaI 1568 TGATCA T^GATCA
FseI 957 GGCCGGCC GGCCGG^CC
HaeII 1931 GGCGCC [GA]GCGC^[CT]
HgaI 2215 GACGC GACGC(5/10)
HincII 889 GTCAAC GT[CT]^[GA]AC
HindII 889 GTCAAC GT[CT]^[GA]AC
KasI 1931 GGCGCC G^GCGCC
MflI 2642 AGATCT [GA]^GATC[CT]
MluNI 500 TGGCCA TGG^CCA
MscI 500 TGGCCA TGG^CCA
MslI 774 CATTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 2449 CGCG CG^CG
NaeI 958 GCCGGC GCC^GGC
NarI 1931 GGCGCC GG^CGCC
NcoI 198 CCATGG C^CATGG
NgoMIV 958 GCCGGC G^CCGGC
NsiI 1002 ATGCAT ATGCA^T
PmaCI 2245 CACGTG CAC^GTG
PmlI 2245 CACGTG CAC^GTG
PvuI 735 CGATCG CGAT^CG
RcaI 258 TCATGA T^CATGA
RsaI 759 GTAC GT^AC
SexAI 2390 ACCTGGT A^CC[AT]GGT
SfoI 1931 GGCGCC GGC^GCC
StuI 1484 AGGCCT AGG^CCT
XhoII 2642 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 165 AAATTT [GA]^AATT[CT]
2824 GAATTT
AcyI 1931 GGCGCC G[GA]^CG[CT]C
1945 GACGTC
ApoI 165 AAATTT [GA]^AATT[CT]
2824 GAATTT
Asp700I 2726 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2747 GAAGTAGTTC
BcnI 1754 CCCGG CC^[GC]GG
2567 CCGGG
BfaI 107 CTAG C^TAG
626 CTAG
BlpI 2284 GCTGAGC GC^T[ACGT]AGC
2404 GCTGAGC
Bpu1102I 2284 GCTGAGC GC^T[ACGT]AGC
2404 GCTGAGC
BsaAI 338 CACGTA [CT]AC^GT[GA]
2245 CACGTG
BsaHI 1931 GGCGCC G[GA]^CG[CT]C
1945 GACGTC
BsrBI 1438 CCGCTC CCGCTC(-3/-3)
2287 GAGCGG
BsrDI 123 CATTGC GCAATG(2/0)
774 CATTGC
BsrFI 958 GCCGGC [GA]^CCGG[CT]
1698 GCCGGT
BssSI 1294 CTCGTG CACGAG(-5/-1)
2494 CTCGTG
CelII 2284 GCTGAGC GC^T[ACGT]AGC
2404 GCTGAGC
Cfr10I 958 GCCGGC [GA]^CCGG[CT]
1698 GCCGGT
DraI 1069 TTTAAA TTT^AAA
1627 TTTAAA
EcoNI 1905 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2616 CCTCTACAAGG
Hin1I 1931 GGCGCC G[GA]^CG[CT]C
1945 GACGTC
MaeI 107 CTAG C^TAG
626 CTAG
NciI 1754 CCCGG CC^[GC]GG
2567 CCGGG
NspI 1004 GCATGC [GA]CATG^[CT]
2328 GCATGC
PstI 895 CTGCAG CTGCA^G
2036 CTGCAG
SacI 1765 GAGCTC GAGCT^C
2692 GAGCTC
SphI 1004 GCATGC GCATG^C
2328 GCATGC
Tsp45I 930 GTCAC ^GT[GC]AC
1537 GTGAC
XcmI 744 CCAGGTGTTGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2018 CCAGAGCTGCCCTGG
XmnI 2726 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2747 GAAGTAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaII
BanI BanII BbsI BbvI BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PflMI PleI PpuMI PvuII
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I Van91I