Restriction map for
pFN21AE1310
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BamHI BbeI BbsI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstYI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoT22I
FbaI FseI FspI HaeII HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MflI
MluI MroI MseI MunI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PstI PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tru9I Tth111I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 22 enzymes) #
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BglI 1091 GCCTCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 105 GTGCAG GTGCAG(16/14)
BsmI 714 GAATGC GAATGC(1/-1)
BsrBI 781 GAGCGG CCGCTC(-3/-3)
BsrDI 237 GCAATG GCAATG(2/0)
BstXI 606 CCACCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII 318 GATC ^GATC
DraIII 79 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 1054 CTCTTC CTCTTC(1/4)
EcoRV 9 GATATC GAT^ATC
FauI 783 GCGGG CCCGC(4/6)
Ksp632I 1054 CTCTTC CTCTTC(1/4)
MboI 318 GATC ^GATC
MspA1I 915 CAGCTG C[AC]G^C[GT]G
NcoI 953 CCATGG C^CATGG
NdeI 1172 CATATG CA^TATG
NdeII 318 GATC ^GATC
PvuII 915 CAGCTG CAG^CTG
Sau3AI 318 GATC ^GATC
SfcI 131 CTGTAG C^T[GA][CT]AG
StuI 680 AGGCCT AGG^CCT
Tsp509I 1168 AATT ^AATT
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# TWO CUT ENZYMES ( 32 enzymes) #
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AccII 181 CGCG CG^CG
252 CGCG
AocI 491 CCTCAGG CC^T[ACGT]AGG
925 CCTAAGG
ApaI 446 GGGCCC GGGCC^C
616 GGGCCC
BalI 35 TGGCCA TGG^CCA
1070 TGGCCA
BanI 301 GGCACC G^G[CT][GA]CC
1182 GGTGCC
BbrPI 102 CACGTG CAC^GTG
585 CACGTG
BbvCI 631 GCTGAGG CCTCAGC(-5/-2)
931 GCTGAGG
BsaAI 102 CACGTG [CT]AC^GT[GA]
585 CACGTG
BstUI 181 CGCG CG^CG
252 CGCG
Bsu36I 491 CCTCAGG CC^T[ACGT]AGG
925 CCTAAGG
CfoI 182 GCGC GCG^C
808 GCGC
DsaI 78 CCACGG C^C[GA][CT]GG
953 CCATGG
EaeI 35 TGGCCA [CT]^GGCC[GA]
1070 TGGCCA
Eco81I 491 CCTCAGG CC^T[ACGT]AGG
925 CCTAAGG
EcoT14I 953 CCATGG C^C[AT][AT]GG
1044 CCTTGG
HhaI 182 GCGC GCG^C
808 GCGC
HinP1I 182 GCGC G^CGC
808 GCGC
HinfI 367 GAGTC G^A[ACGT]TC
420 GACTC
HphI 327 GGTGA GGTGA(8/7)
1079 TCACC
MaeIII 85 GTGAC ^GT[ACGT]AC
481 GTGAC
MboII 427 TCTTC GAAGA(8/7)
1055 TCTTC
MluNI 35 TGGCCA TGG^CCA
1070 TGGCCA
MscI 35 TGGCCA TGG^CCA
1070 TGGCCA
MslI 76 CACCACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
811 CACACGGATG
MvnI 181 CGCG CG^CG
252 CGCG
PflMI 620 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
963 CCAGAGGCTGG
PleI 420 GACTC GAGTC(4/5)
367 GAGTC
PmaCI 102 CACGTG CAC^GTG
585 CACGTG
PmlI 102 CACGTG CAC^GTG
585 CACGTG
StyI 953 CCATGG C^C[AT][AT]GG
1044 CCTTGG
Tsp45I 85 GTGAC ^GT[GC]AC
481 GTGAC
Van91I 620 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
963 CCAGAGGCTGG
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AcyI AluI AlwNI AvaII
BanII BbvI BcnI BmyI BpmI
BsaHI BsaJI BseRI BsiYI BslI
BsmAI BsmBI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DraII EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HgaI Hin1I
HpaII ItaI MaeII MnlI MspI
MvaI MwoI NciI NlaIII NlaIV
PpuMI Sau96I ScrFI SfaNI TaqI
TseI TspRI TthHB8I