Restriction map for
pFN21AE1384
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
AocI Aor51HI ApoI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BamHI BbeI BbsI BclI
BfaI BfrI BglI BlnI BpuAI
BsaBI BsaHI BsaI BseAI BsiWI
BsmAI BsmBI BsmI BspDI BspEI
BspHI BsrDI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI MaeI MamI MfeI
MluI MroI MseI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI TfiI Tru9I Tsp509I
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccII 1562 CGCG CG^CG
AflIII 847 ACATGT A^C[GA][CT]GT
AlwI 1269 GATCC GGATC(4/5)
Asp700I 621 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 110 TGGCCA TGG^CCA
BbrPI 1612 CACGTG CAC^GTG
BglII 1769 AGATCT A^GATCT
BlpI 1282 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1282 GCTGAGC GC^T[ACGT]AGC
BsaAI 1612 CACGTG [CT]AC^GT[GA]
BsaWI 1231 TCCGGT [AT]^CCGG[AT]
BsiEI 1539 CGACCG CG[GA][CT]^CG
Bsp1407I 1206 TGTACA T^GTACA
BspLU11I 847 ACATGT A^CATGT
BspMI 1223 ACCTGC ACCTGC(4/8)
BsrGI 1206 TGTACA T^GTACA
BstUI 1562 CGCG CG^CG
CelII 1282 GCTGAGC GC^T[ACGT]AGC
EarI 1336 CTCTTC CTCTTC(1/4)
EcoNI 1463 CCTTCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Ksp632I 1336 CTCTTC CTCTTC(1/4)
KspI 1561 CCGCGG CCGC^GG
MluNI 110 TGGCCA TGG^CCA
MscI 110 TGGCCA TGG^CCA
MvnI 1562 CGCG CG^CG
NaeI 1040 GCCGGC GCC^GGC
NcoI 101 CCATGG C^CATGG
NgoMIV 1040 GCCGGC G^CCGGC
NspI 847 ACATGT [GA]CATG^[CT]
PmaCI 1612 CACGTG CAC^GTG
PmlI 1612 CACGTG CAC^GTG
SacI 1333 GAGCTC GAGCT^C
SacII 1561 CCGCGG CCGC^GG
SapI 1335 GCTCTTC GCTCTTC(1/4)
SspBI 1206 TGTACA T^GTACA
XmnI 621 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Alw44I 1109 GTGCAC G^TGCAC
1695 GTGCAC
ApaLI 1109 GTGCAC G^TGCAC
1695 GTGCAC
BanI 699 GGCACC G^G[CT][GA]CC
1743 GGTGCC
BbvCI 236 CCTCAGC CCTCAGC(-5/-2)
272 CCTCAGC
BcgI 946 CGAAACCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1494 CGATGTCCCTGC
BsgI 1477 CTGCAC GTGCAG(16/14)
883 GTGCAG
BsrBI 348 GAGCGG CCGCTC(-3/-3)
768 GAGCGG
BstXI 101 CCATGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
828 CCAGCATTATGG
BstYI 1268 AGATCC [GA]^GATC[CT]
1769 AGATCT
CfoI 876 GCGC GCG^C
1713 GCGC
DpnII 1269 GATC ^GATC
1770 GATC
EaeI 110 TGGCCA [CT]^GGCC[GA]
474 CGGCCA
EcoT14I 101 CCATGG C^C[AT][AT]GG
758 CCAAGG
HhaI 876 GCGC GCG^C
1713 GCGC
HinP1I 876 GCGC G^CGC
1713 GCGC
MaeII 1613 ACGT A^CGT
1659 ACGT
MboI 1269 GATC ^GATC
1770 GATC
MflI 1268 AGATCC [GA]^GATC[CT]
1769 AGATCT
MslI 162 CACCTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
832 CATTATGGTG
NdeII 1269 GATC ^GATC
1770 GATC
PflMI 645 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1161 CCAGGAGCTGG
Sau3AI 1269 GATC ^GATC
1770 GATC
StuI 504 AGGCCT AGG^CCT
1398 AGGCCT
StyI 101 CCATGG C^C[AT][AT]GG
758 CCAAGG
TaqI 308 TCGA T^CGA
1493 TCGA
TthHB8I 308 TCGA T^CGA
1493 TCGA
Van91I 645 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1161 CCAGGAGCTGG
XhoII 1268 AGATCC [GA]^GATC[CT]
1769 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AfaI AluI AlwNI ApaI
AspHI AvaI AvaII BanII BbvI
BciVI BcnI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstAPI BstF5I BstNI Cac8I Cfr10I
Cfr13I DdeI DraII DsaI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeIII MboII MnlI MspA1I MspI
MvaI MwoI NciI NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
Sau96I ScrFI SfaNI SfcI SmlI
TseI Tsp45I TspRI XcmI