Restriction map for
pFN21AE5210
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# NO CUT ENZYMES ( 181 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsiHKAI BsiWI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BsrI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII CfoI
ClaI CpoI DpnII DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII HpaI HphI KasI
KpnI KspI MaeI MamI MboI
MfeI MflI MluI MluNI MroI
MscI MseI MslI MspA1I MunI
MvnI NaeI NarI NdeI NdeII
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
Sau3AI SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Tru9I Tth111I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 466 GTAGAC GT^[AC][GT]AC
AcsI 214 AAATTC [GA]^AATT[CT]
ApoI 214 AAATTC [GA]^AATT[CT]
BanI 97 GGCACC G^G[CT][GA]CC
BbvI 436 GCAGC GCAGC(8/12)
BsaI 22 GAGACC GGTCTC(1/5)
BseRI 19 GAGGAG GAGGAG(10/8)
BsiEI 123 CGACCG CG[GA][CT]^CG
BsiYI 384 CCAGGGCGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 384 CCAGGGCGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 529 GAGACG CGTCTC(1/5)
BspMI 202 ACCTGC ACCTGC(4/8)
BsrFI 86 ACCGGC [GA]^CCGG[CT]
BstAPI 268 GCACCTTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 414 CATCC GGATG(2/0)
Cac8I 159 GCTGGC GC[ACGT]^[ACGT]GC
Cfr10I 86 ACCGGC [GA]^CCGG[CT]
DdeI 377 CTCAG C^T[ACGT]AG
DraII 199 GGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 161 TGGCCG [CT]^GGCC[GA]
EcoO109I 199 GGGACCT [GA]G^G[ACGT]CC[CT]
FokI 414 CATCC GGATG(9/13)
HincII 534 GTTGAC GT[CT]^[GA]AC
HindII 534 GTTGAC GT[CT]^[GA]AC
MaeIII 247 GTCAC ^GT[ACGT]AC
MnlI 19 GAGG CCTC(7/6)
PflMI 384 CCAGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 199 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 24 GACCCGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 456 CTGCAG CTGCA^G
SfcI 456 CTGCAG C^T[GA][CT]AG
SspI 169 AATATT AAT^ATT
TaqI 357 TCGA T^CGA
TseI 436 GCAGC G^C[AT]GC
Tsp45I 247 GTCAC ^GT[GC]AC
Tsp509I 215 AATT ^AATT
TspRI 363 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 357 TCGA T^CGA
Van91I 384 CCAGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 473 CCATGGCGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
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AvaII 84 GGACC G^G[AT]CC
200 GGACC
BcnI 126 CCGGG CC^[GC]GG
176 CCGGG
BsgI 115 CTGCAC GTGCAG(16/14)
305 GTGCAG
BsmAI 22 GAGAC GTCTC(1/5)
529 GAGAC
BsmFI 179 GGGAC GGGAC(10/14)
199 GGGAC
BstNI 301 CCTGG CC^[AT]GG
384 CCAGG
DsaI 404 CCATGG C^C[GA][CT]GG
473 CCATGG
EarI 16 GAAGAG CTCTTC(1/4)
526 GAAGAG
EcoRII 301 CCTGG ^CC[AT]GG
384 CCAGG
EcoT14I 404 CCATGG C^C[AT][AT]GG
473 CCATGG
HinfI 66 GAATC G^A[ACGT]TC
359 GAATC
Ksp632I 16 GAAGAG CTCTTC(1/4)
526 GAAGAG
MaeII 245 ACGT A^CGT
532 ACGT
MvaI 301 CCTGG CC^[AT]GG
384 CCAGG
MwoI 186 GCTGGTGTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
268 GCACCTTATGC
NciI 126 CCGGG CC^[GC]GG
176 CCGGG
NcoI 404 CCATGG C^CATGG
473 CCATGG
StyI 404 CCATGG C^C[AT][AT]GG
473 CCATGG
TfiI 66 GAATC G^A[AT]TC
359 GAATC
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# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AciI AfaI AluI BsaJI BssKI
Cfr13I Fnu4HI HaeIII HapII HpaII
ItaI MboII MspI NlaIII NlaIV
RsaI Sau96I ScrFI