Restriction map for
pFN21AE3603
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# NO CUT ENZYMES ( 156 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AflIII AhdI AlwI AlwNI
AocI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaBI
BsaI BseAI BsgI BsiEI BsiWI
BsmBI BsmI BsoBI BspDI BspEI
BspLU11I BsrBI BsrDI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DdeI DpnII DraI DraIII
DrdI DsaI EagI Eam1105I EarI
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoT22I FbaI FseI FspI
HgaI HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MamI MboI MfeI MflI MluI
MroI MslI MunI NaeI NarI
NcoI NdeI NdeII NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
Sau3AI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TfiI Tsp509I Tth111I Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 571 GACGTC GACGT^C
AccII 138 CGCG CG^CG
AciI 10 CCGC CCGC(-3/-1)
AcyI 571 GACGTC G[GA]^CG[CT]C
AfaI 27 GTAC GT^AC
AgeI 354 ACCGGT A^CCGGT
Alw44I 88 GTGCAC G^TGCAC
Aor51HI 80 AGCGCT AGC^GCT
ApaLI 88 GTGCAC G^TGCAC
BalI 374 TGGCCA TGG^CCA
BpmI 507 CTCCAG CTGGAG(16/14)
BsaAI 85 TACGTG [CT]AC^GT[GA]
BsaHI 571 GACGTC G[GA]^CG[CT]C
BsaWI 354 ACCGGT [AT]^CCGG[AT]
BseRI 102 CTCCTC GAGGAG(10/8)
BsmFI 158 GGGAC GGGAC(10/14)
Bsp1407I 26 TGTACA T^GTACA
BspHI 407 TCATGA T^CATGA
BspMI 199 GCAGGT ACCTGC(4/8)
BsrFI 354 ACCGGT [GA]^CCGG[CT]
BsrGI 26 TGTACA T^GTACA
BstUI 138 CGCG CG^CG
BstXI 509 CCAGATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 354 ACCGGT [GA]^CCGG[CT]
EaeI 374 TGGCCA [CT]^GGCC[GA]
Eco47III 80 AGCGCT AGC^GCT
EcoRV 193 GATATC GAT^ATC
EcoT14I 599 CCAAGG C^C[AT][AT]GG
FauI 9 CCCGC CCCGC(4/6)
HaeII 80 AGCGCT [GA]GCGC^[CT]
Hin1I 571 GACGTC G[GA]^CG[CT]C
MluNI 374 TGGCCA TGG^CCA
MscI 374 TGGCCA TGG^CCA
MseI 334 TTAA T^TAA
MspA1I 609 CAGCTG C[AC]G^C[GT]G
MvnI 138 CGCG CG^CG
PinAI 354 ACCGGT A^CCGGT
PpuMI 158 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 225 GACGAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 181 CTGCAG CTGCA^G
PvuII 609 CAGCTG CAG^CTG
RcaI 407 TCATGA T^CATGA
RsaI 27 GTAC GT^AC
SfaNI 512 GATGC GCATC(5/9)
SfcI 181 CTGCAG C^T[GA][CT]AG
SspBI 26 TGTACA T^GTACA
StyI 599 CCAAGG C^C[AT][AT]GG
TaqI 269 TCGA T^CGA
Tru9I 334 TTAA T^TAA
TthHB8I 269 TCGA T^CGA
XcmI 119 CCACAATCGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
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AvaII 159 GGACC G^G[AT]CC
429 GGACC
BbsI 63 GAAGAC GAAGAC(2/6)
265 GTCTTC
BbvI 183 GCAGC GCAGC(8/12)
611 GCTGC
BcnI 114 CCGGG CC^[GC]GG
162 CCCGG
BpuAI 63 GAAGAC GAAGAC(2/6)
265 GTCTTC
BsmAI 166 GAGAC GTCTC(1/5)
338 GTCTC
BstF5I 399 GGATG GGATG(2/0)
460 GGATG
DraII 5 AGGCCCC [GA]G^G[ACGT]CC[CT]
158 GGGACCC
EcoO109I 5 AGGCCCC [GA]G^G[ACGT]CC[CT]
158 GGGACCC
Fnu4HI 183 GCAGC GC^[ACGT]GC
611 GCTGC
FokI 399 GGATG GGATG(9/13)
460 GGATG
HinfI 325 GACTC G^A[ACGT]TC
556 GAGTC
ItaI 183 GCAGC GC^[ACGT]GC
611 GCTGC
MaeII 86 ACGT A^CGT
572 ACGT
MboII 63 GAAGA GAAGA(8/7)
266 TCTTC
NciI 114 CCGGG CC^[GC]GG
162 CCCGG
NlaIII 222 CATG CATG^
408 CATG
PleI 325 GACTC GAGTC(4/5)
556 GAGTC
SmlI 142 CTCAAG C^T[CT][GA]AG
421 CTCAAG
TseI 183 GCAGC G^C[AT]GC
611 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AluI AspHI BmyI BsaJI BsiHKAI
BsiYI BslI Bsp1286I BsrI BssKI
BstNI Cac8I CfoI Cfr13I EcoRII
HaeIII HapII HhaI HinP1I HpaII
HphI MaeIII MnlI MspI MvaI
MwoI NlaIV Sau96I ScrFI Tsp45I
TspRI