Restriction map for
pFN21AE2247
##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI Aor51HI AscI AseI AsnI
Asp718I AviII AvrII BbvCI BcgI
BciVI BfrI BlnI BsaBI BsiWI
BsmBI BsmI Bsp1407I BspDI BsrDI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I ClaI DraI EagI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV FseI FspI HindIII HpaI
KpnI KspI MamI MfeI MluI
MseI MunI NaeI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI SacII SalI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SspBI SspI SwaI Tru9I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AcsI 2371 GAATTT [GA]^AATT[CT]
AocI 1789 CCTCAGG CC^T[ACGT]AGG
ApoI 2371 GAATTT [GA]^AATT[CT]
Asp700I 2244 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2479 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 959 GGCGCC GGCGC^C
BbrPI 1816 CACGTG CAC^GTG
BclI 2055 TGATCA T^GATCA
BfaI 728 CTAG C^TAG
BsiEI 694 CGACCG CG[GA][CT]^CG
BspLU11I 2636 ACATGT A^CATGT
BsrBI 1492 GAGCGG CCGCTC(-3/-3)
BssSI 1216 CACGAG CACGAG(-5/-1)
Bsu36I 1789 CCTCAGG CC^T[ACGT]AGG
CpoI 1495 CGGACCG CG^G[AT]CCG
DraIII 1406 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 717 GACTTACGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1789 CCTCAGG CC^T[ACGT]AGG
FbaI 2055 TGATCA T^GATCA
HgaI 311 GCGTC GACGC(5/10)
KasI 959 GGCGCC G^GCGCC
MaeI 728 CTAG C^TAG
NarI 959 GGCGCC GG^CGCC
PflFI 2479 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2481 CCAAGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1816 CACGTG CAC^GTG
PmlI 1816 CACGTG CAC^GTG
RsrII 1495 CGGACCG CG^G[AT]CCG
SacI 1378 GAGCTC GAGCT^C
SapI 2025 GAAGAGC GCTCTTC(1/4)
SbfI 1593 CCTGCAGG CCTGCA^GG
ScaI 2573 AGTACT AGT^ACT
SfoI 959 GGCGCC GGC^GCC
SmaI 2210 CCCGGG CCC^GGG
SmlI 1300 CTCAAG C^T[CT][GA]AG
Sse8387I 1593 CCTGCAGG CCTGCA^GG
TfiI 13 GATTC G^A[AT]TC
Tsp509I 2372 AATT ^AATT
Tth111I 2479 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2481 CCAAGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 2210 CCCGGG C^CCGGG
XmaI 2210 CCCGGG C^CCGGG
XmnI 2244 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccIII 134 TCCGGA T^CCGGA
1157 TCCGGA
AcyI 310 GGCGTC G[GA]^CG[CT]C
959 GGCGCC
AflIII 722 ACGTGT A^C[GA][CT]GT
2636 ACATGT
AhdI 2440 GACTTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2632 GACTACATGTC
Alw44I 259 GTGCAC G^TGCAC
1612 GTGCAC
ApaI 1460 GGGCCC GGGCC^C
1927 GGGCCC
ApaLI 259 GTGCAC G^TGCAC
1612 GTGCAC
AspEI 2440 GACTTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2632 GACTACATGTC
BamHI 866 GGATCC G^GATCC
1934 GGATCC
BbsI 673 GAAGAC GAAGAC(2/6)
829 GAAGAC
BglI 754 GCCCAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1543 GCCAAGCGGGC
BglII 266 AGATCT A^GATCT
1433 AGATCT
BlpI 1038 GCTCAGC GC^T[ACGT]AGC
2001 GCTCAGC
Bpu1102I 1038 GCTCAGC GC^T[ACGT]AGC
2001 GCTCAGC
BpuAI 673 GAAGAC GAAGAC(2/6)
829 GAAGAC
BsaAI 721 TACGTG [CT]AC^GT[GA]
1816 CACGTG
BsaHI 310 GGCGTC G[GA]^CG[CT]C
959 GGCGCC
BseAI 134 TCCGGA T^CCGGA
1157 TCCGGA
BspEI 134 TCCGGA T^CCGGA
1157 TCCGGA
BspHI 713 TCATGA T^CATGA
2384 TCATGA
BstXI 656 CCAAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2402 CCAACCGCCTGG
CelII 1038 GCTCAGC GC^T[ACGT]AGC
2001 GCTCAGC
Eam1105I 2440 GACTTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2632 GACTACATGTC
EcoNI 132 CCTCCGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1110 CCTGGAGCAGG
EcoT22I 916 ATGCAT ATGCA^T
1978 ATGCAT
Hin1I 310 GGCGTC G[GA]^CG[CT]C
959 GGCGCC
MroI 134 TCCGGA T^CCGGA
1157 TCCGGA
NsiI 916 ATGCAT ATGCA^T
1978 ATGCAT
PleI 1402 GACTC GAGTC(4/5)
2611 GACTC
PstI 1021 CTGCAG CTGCA^G
1594 CTGCAG
RcaI 713 TCATGA T^CATGA
2384 TCATGA
SfcI 1021 CTGCAG C^T[GA][CT]AG
1594 CTGCAG
SphI 918 GCATGC GCATG^C
1863 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BalI
BanI BanII BbvI BcnI BmyI
BpmI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI StuI StyI TaqI
TseI Tsp45I TspRI TthHB8I XcmI
XhoII