Restriction map for
pFN21AE4532
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BbeI BbrPI
BcgI BciVI BclI BfrI BglII
BlnI BsaAI BsaBI BsaHI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI Bsu36I Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoRI FbaI FseI FspI
Hin1I HincII HindII HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NheI NotI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccII 528 CGCG CG^CG
AflIII 306 ACACGT A^C[GA][CT]GT
ApaI 663 GGGCCC GGGCC^C
AvaI 362 CCCGAG C^[CT]CG[GA]G
BalI 1115 TGGCCA TGG^CCA
BamHI 1702 GGATCC G^GATCC
BanI 1078 GGCACC G^G[CT][GA]CC
BfaI 935 CTAG C^TAG
BglI 595 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsoBI 362 CCCGAG C^[CT]CG[GA]G
BspHI 149 TCATGA T^CATGA
BspMI 1040 ACCTGC ACCTGC(4/8)
Bst1107I 787 GTATAC GTA^TAC
BstEII 1665 GGTGACC G^GT[ACGT]ACC
BstPI 1665 GGTGACC G^GT[ACGT]ACC
BstUI 528 CGCG CG^CG
BstXI 1175 CCATTCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1702 GGATCC [GA]^GATC[CT]
BstZ17I 787 GTATAC GTA^TAC
DsaI 1118 CCATGG C^C[GA][CT]GG
EaeI 1115 TGGCCA [CT]^GGCC[GA]
EcoO65I 1665 GGTGACC G^GT[ACGT]ACC
EcoRV 178 GATATC GAT^ATC
EcoT22I 43 ATGCAT ATGCA^T
HgaI 1368 GCGTC GACGC(5/10)
HindIII 462 AAGCTT A^AGCTT
MaeI 935 CTAG C^TAG
MaeII 308 ACGT A^CGT
MflI 1702 GGATCC [GA]^GATC[CT]
MluNI 1115 TGGCCA TGG^CCA
MscI 1115 TGGCCA TGG^CCA
MspA1I 804 CAGCGG C[AC]G^C[GT]G
MvnI 528 CGCG CG^CG
NcoI 1118 CCATGG C^CATGG
NruI 527 TCGCGA TCG^CGA
NsiI 43 ATGCAT ATGCA^T
PshAI 322 GACTCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 149 TCATGA T^CATGA
SacI 1018 GAGCTC GAGCT^C
ScaI 1028 AGTACT AGT^ACT
SfcI 468 CTACAG C^T[GA][CT]AG
SspI 400 AATATT AAT^ATT
Tsp509I 737 AATT ^AATT
XhoII 1702 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AccI 787 GTATAC GT^[AC][GT]AC
1084 GTAGAC
BanII 663 GGGCCC G[GA]GC[CT]^C
1018 GAGCTC
BbsI 960 GAAGAC GAAGAC(2/6)
310 GTCTTC
BbvCI 1051 GCTGAGG CCTCAGC(-5/-2)
1132 GCTGAGG
BlpI 604 GCTCAGC GC^T[ACGT]AGC
1020 GCTCAGC
Bpu1102I 604 GCTCAGC GC^T[ACGT]AGC
1020 GCTCAGC
BpuAI 960 GAAGAC GAAGAC(2/6)
310 GTCTTC
BsaI 799 GAGACC GGTCTC(1/5)
914 GGTCTC
BsgI 302 CTGCAC GTGCAG(16/14)
1042 CTGCAC
BsmAI 799 GAGAC GTCTC(1/5)
915 GTCTC
BsrBI 199 CCGCTC CCGCTC(-3/-3)
1387 GAGCGG
CelII 604 GCTCAGC GC^T[ACGT]AGC
1020 GCTCAGC
EcoNI 840 CCTGGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1047 CCTTGCTGAGG
HaeII 509 AGCGCC [GA]GCGC^[CT]
1546 AGCGCC
MslI 111 CATGAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1320 CACCAACATG
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BbvI BcnI BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeIII MboI MboII MnlI
MseI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TfiI Tru9I TseI
Tsp45I TspRI TthHB8I