Restriction map for
pFN21AB8241
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Alw44I AocI ApaLI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BseAI BsiWI Bsp1407I BspDI BspEI
BspLU11I BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI Eam1105I EarI Eco81I
EcoO65I EcoRV EcoT22I FbaI FspI
HgaI HindIII HpaI Ksp632I KspI
MaeI MamI MfeI MluI MroI
MseI MunI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI TfiI Tru9I Tth111I Van91I
XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 775 GGTACC G^GTACC
AccI 966 GTCTAC GT^[AC][GT]AC
AcsI 481 GAATTC [GA]^AATT[CT]
AcyI 448 GGCGCC G[GA]^CG[CT]C
AflIII 581 ACGTGT A^C[GA][CT]GT
AlwNI 1306 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 630 AGCGCT AGC^GCT
ApaI 214 GGGCCC GGGCC^C
ApoI 481 GAATTC [GA]^AATT[CT]
Asp718I 775 GGTACC G^GTACC
AvaI 1336 CTCGAG C^[CT]CG[GA]G
BamHI 224 GGATCC G^GATCC
BbeI 448 GGCGCC GGCGC^C
BbsI 112 GAAGAC GAAGAC(2/6)
BglII 386 AGATCT A^GATCT
BpmI 150 CTGGAG CTGGAG(16/14)
BpuAI 112 GAAGAC GAAGAC(2/6)
BsaHI 448 GGCGCC G[GA]^CG[CT]C
BsaWI 116 ACCGGA [AT]^CCGG[AT]
BsmBI 997 GAGACG CGTCTC(1/5)
BsmI 552 GAATGC GAATGC(1/-1)
BsoBI 1336 CTCGAG C^[CT]CG[GA]G
BspHI 302 TCATGA T^CATGA
BsrFI 1215 GCCGGC [GA]^CCGG[CT]
BssSI 1126 CACGAG CACGAG(-5/-1)
Cfr10I 1215 GCCGGC [GA]^CCGG[CT]
Eco47III 630 AGCGCT AGC^GCT
EcoNI 204 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 481 GAATTC G^AATTC
EcoT14I 443 CCATGG C^C[AT][AT]GG
FseI 1214 GGCCGGCC GGCCGG^CC
Hin1I 448 GGCGCC G[GA]^CG[CT]C
HincII 844 GTCAAC GT[CT]^[GA]AC
HindII 844 GTCAAC GT[CT]^[GA]AC
KasI 448 GGCGCC G^GCGCC
KpnI 775 GGTACC GGTAC^C
MboII 112 GAAGA GAAGA(8/7)
MslI 717 CACAACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1215 GCCGGC GCC^GGC
NarI 448 GGCGCC GG^CGCC
NcoI 443 CCATGG C^CATGG
NgoMIV 1215 GCCGGC G^CCGGC
NspI 906 GCATGC [GA]CATG^[CT]
PaeR7I 1336 CTCGAG C^TCGAG
PstI 505 CTGCAG CTGCA^G
PvuII 1052 CAGCTG CAG^CTG
RcaI 302 TCATGA T^CATGA
SacI 1333 GAGCTC GAGCT^C
SexAI 778 ACCTGGT A^CC[AT]GGT
SfcI 505 CTGCAG C^T[GA][CT]AG
SfoI 448 GGCGCC GGC^GCC
SphI 906 GCATGC GCATG^C
StyI 443 CCATGG C^C[AT][AT]GG
Tsp509I 482 AATT ^AATT
XhoI 1336 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AspHI 1274 GTGCTC G[AT]GC[AT]^C
1333 GAGCTC
AvaII 378 GGACC G^G[AT]CC
1071 GGACC
BanII 214 GGGCCC G[GA]GC[CT]^C
1333 GAGCTC
BsgI 1027 GTGCAG GTGCAG(16/14)
1303 GTGCAG
BsiHKAI 1274 GTGCTC G[AT]GC[AT]^C
1333 GAGCTC
BsmFI 932 GGGAC GGGAC(10/14)
750 GTCCC
BsrBI 1074 CCGCTC CCGCTC(-3/-3)
1342 GAGCGG
BstXI 329 CCAAGTATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
716 CCACAACTGTGG
DraII 213 GGGGCCC [GA]G^G[ACGT]CC[CT]
604 GGGGCCT
EcoO109I 213 GGGGCCC [GA]G^G[ACGT]CC[CT]
604 GGGGCCT
SmlI 289 CTCAAG C^T[CT][GA]AG
1336 CTCGAG
XcmI 329 CCAAGTATCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
500 CCAATCTGCAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
BalI BanI BbvI BcnI BglI
BmyI BsaJI BseRI BsiEI BsiYI
BslI BsmAI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EaeI EagI EclXI Eco52I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MflI MluNI MnlI MscI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
StuI TaqI TseI Tsp45I TspRI
TthHB8I XhoII