Restriction map for
pFN21AE5604
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# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII AccI AclI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AseI AsnI AspEI
AspI AviII BalI BamHI BbrPI
BbsI BbvCI BciVI BfrI BglII
BpuAI BsaAI BsaBI BseRI BsiWI
BsmI BspDI BspHI BspLU11I BspMI
BsrDI BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DrdI Eam1105I Eco47III Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HpaI KspI MamI MfeI
MluI MluNI MscI MslI MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SbfI ScaI
SfcI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tth111I Van91I XbaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccIII 757 TCCGGA T^CCGGA
AlwNI 538 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
AscI 348 GGCGCGCC GG^CGCGCC
AvrII 435 CCTAGG C^CTAGG
BcgI 123 GCAGAAGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 156 CCCGG CC^[GC]GG
BlnI 435 CCTAGG C^CTAGG
BlpI 594 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 594 GCTGAGC GC^T[ACGT]AGC
BsaI 359 GAGACC GGTCTC(1/5)
BseAI 757 TCCGGA T^CCGGA
BsgI 610 GTGCAG GTGCAG(16/14)
BsmBI 160 GAGACG CGTCTC(1/5)
Bsp1407I 1105 TGTACA T^GTACA
BspEI 757 TCCGGA T^CCGGA
BsrGI 1105 TGTACA T^GTACA
BssHII 349 GCGCGC G^CGCGC
BstAPI 603 GCAGAGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 709 GGATG GGATG(2/0)
BstXI 699 CCAGCGTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 730 GGATCT [GA]^GATC[CT]
CelII 594 GCTGAGC GC^T[ACGT]AGC
DraI 1177 TTTAAA TTT^AAA
EagI 369 CGGCCG C^GGCCG
EclXI 369 CGGCCG C^GGCCG
Eco52I 369 CGGCCG C^GGCCG
FokI 709 GGATG GGATG(9/13)
HgaI 588 GACGC GACGC(5/10)
HincII 780 GTTGAC GT[CT]^[GA]AC
HindII 780 GTTGAC GT[CT]^[GA]AC
HindIII 1063 AAGCTT A^AGCTT
MaeII 320 ACGT A^CGT
MflI 730 GGATCT [GA]^GATC[CT]
MroI 757 TCCGGA T^CCGGA
NaeI 599 GCCGGC GCC^GGC
NciI 156 CCCGG CC^[GC]GG
NgoMIV 599 GCCGGC G^CCGGC
NspI 1005 GCATGT [GA]CATG^[CT]
PaeR7I 131 CTCGAG C^TCGAG
PpuMI 1029 GGGTCCT [GA]G^G[AT]CC[CT]
SapI 189 GCTCTTC GCTCTTC(1/4)
SspBI 1105 TGTACA T^GTACA
StuI 674 AGGCCT AGG^CCT
Tsp45I 572 GTCAC ^GT[GC]AC
XcmI 344 CCACGGCGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 131 CTCGAG C^TCGAG
XhoII 730 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 1198 GAATTT [GA]^AATT[CT]
1401 AAATTT
AcyI 6 GGCGCC G[GA]^CG[CT]C
340 GGCGCC
AlwI 1210 GATCC GGATC(4/5)
730 GGATC
ApoI 1198 GAATTT [GA]^AATT[CT]
1401 AAATTT
Asp700I 954 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1198 GAATTTTTTC
BbeI 6 GGCGCC GGCGC^C
340 GGCGCC
BsaHI 6 GGCGCC G[GA]^CG[CT]C
340 GGCGCC
BsaWI 757 TCCGGA [AT]^CCGG[AT]
927 ACCGGA
BsiEI 195 CGACCG CG[GA][CT]^CG
369 CGGCCG
BsrBI 308 GAGCGG CCGCTC(-3/-3)
366 GAGCGG
BsrFI 35 GCCGGT [GA]^CCGG[CT]
599 GCCGGC
Cfr10I 35 GCCGGT [GA]^CCGG[CT]
599 GCCGGC
DraII 446 AGGCCCT [GA]G^G[ACGT]CC[CT]
1029 GGGTCCT
DraIII 316 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
541 CACCTGGTG
EaeI 369 CGGCCG [CT]^GGCC[GA]
647 TGGCCG
EarI 190 CTCTTC CTCTTC(1/4)
694 CTCTTC
EcoO109I 446 AGGCCCT [GA]G^G[ACGT]CC[CT]
1029 GGGTCCT
FauI 310 GCGGG CCCGC(4/6)
1026 GCGGG
Hin1I 6 GGCGCC G[GA]^CG[CT]C
340 GGCGCC
KasI 6 GGCGCC G^GCGCC
340 GGCGCC
Ksp632I 190 CTCTTC CTCTTC(1/4)
694 CTCTTC
NarI 6 GGCGCC GG^CGCC
340 GGCGCC
PleI 253 GACTC GAGTC(4/5)
1298 GAGTC
SexAI 542 ACCTGGT A^CC[AT]GGT
662 ACCAGGT
SfaNI 710 GATGC GCATC(5/9)
1135 GATGC
SfoI 6 GGCGCC GGC^GCC
340 GGCGCC
XmnI 954 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1198 GAATTTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
Acc65I AccII AciI AfaI AluI
Asp718I AspHI AvaI AvaII BanI
BanII BbvI BclI BfaI BglI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EcoRII EcoT14I FbaI Fnu4HI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI KpnI MaeI
MaeIII MboI MboII MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV PvuII RsaI
SacI Sau3AI Sau96I ScrFI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I