Restriction map for
pFN21AE4587
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# NO CUT ENZYMES ( 84 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AgeI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI Asp718I
AvrII BamHI BciVI BclI BfrI
BglII BlnI BsaBI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI Bst1107I BstBI
BstZ17I ClaI DraI Eco47III EcoRI
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KpnI MamI MfeI
MluI MroI MseI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI SalI
SapI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI SwaI Tru9I Tsp509I
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 1573 GACGTC GACGT^C
AflIII 71 ACGTGT A^C[GA][CT]GT
AhdI 1915 GACGTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1471 CCTGAGG CC^T[ACGT]AGG
Asp700I 2376 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1915 GACGTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 143 TGCGCA TGC^GCA
BbeI 1347 GGCGCC GGCGC^C
BfaI 1144 CTAG C^TAG
BglI 1838 GCCCCGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiEI 2055 CGGCCG CG[GA][CT]^CG
BsmBI 1669 GAGACG CGTCTC(1/5)
BsrBI 2065 GAGCGG CCGCTC(-3/-3)
BsrFI 2057 GCCGGC [GA]^CCGG[CT]
BssHII 1194 GCGCGC G^CGCGC
BssSI 790 CACGAG CACGAG(-5/-1)
BstXI 140 CCATGCGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1471 CCTGAGG CC^T[ACGT]AGG
Cfr10I 2057 GCCGGC [GA]^CCGG[CT]
CpoI 864 CGGACCG CG^G[AT]CCG
DraIII 2659 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1870 GACCAGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2055 CGGCCG C^GGCCG
Eam1105I 1915 GACGTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2055 CGGCCG C^GGCCG
Eco52I 2055 CGGCCG C^GGCCG
Eco81I 1471 CCTGAGG CC^T[ACGT]AGG
EcoNI 1017 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2613 GATATC GAT^ATC
FspI 143 TGCGCA TGC^GCA
KasI 1347 GGCGCC G^GCGCC
MaeI 1144 CTAG C^TAG
NaeI 2057 GCCGGC GCC^GGC
NarI 1347 GGCGCC GG^CGCC
NgoMIV 2057 GCCGGC G^CCGGC
NspI 1217 ACATGC [GA]CATG^[CT]
PaeR7I 262 CTCGAG C^TCGAG
PleI 2179 GAGTC GAGTC(4/5)
RsrII 864 CGGACCG CG^G[AT]CCG
SacI 2272 GAGCTC GAGCT^C
SbfI 333 CCTGCAGG CCTGCA^GG
SfoI 1347 GGCGCC GGC^GCC
Sse8387I 333 CCTGCAGG CCTGCA^GG
StuI 2246 AGGCCT AGG^CCT
XbaI 1143 TCTAGA T^CTAGA
XhoI 262 CTCGAG C^TCGAG
XmnI 2376 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AspHI 2151 GAGCAC G[AT]GC[AT]^C
2272 GAGCTC
BanI 1347 GGCGCC G^G[CT][GA]CC
2664 GGTGCC
BbrPI 775 CACGTG CAC^GTG
2659 CACGTG
BbsI 1441 GTCTTC GAAGAC(2/6)
1879 GTCTTC
BbvCI 999 GCTGAGG CCTCAGC(-5/-2)
2362 GCTGAGG
BcgI 2178 CGAGTCCCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1715 GCAAAGCCATCG
BlpI 2269 GCTGAGC GC^T[ACGT]AGC
2350 GCTCAGC
Bpu1102I 2269 GCTGAGC GC^T[ACGT]AGC
2350 GCTCAGC
BpuAI 1441 GTCTTC GAAGAC(2/6)
1879 GTCTTC
BsaI 1819 GAGACC GGTCTC(1/5)
1044 GGTCTC
BsiHKAI 2151 GAGCAC G[AT]GC[AT]^C
2272 GAGCTC
BstAPI 536 GCAAGGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
987 GCAGGGGCTGC
BstEII 150 GGTGACC G^GT[ACGT]ACC
591 GGTCACC
BstPI 150 GGTGACC G^GT[ACGT]ACC
591 GGTCACC
BstYI 266 AGATCC [GA]^GATC[CT]
2075 AGATCC
CelII 2269 GCTGAGC GC^T[ACGT]AGC
2350 GCTCAGC
EarI 2521 CTCTTC CTCTTC(1/4)
10 GAAGAG
EcoO65I 150 GGTGACC G^GT[ACGT]ACC
591 GGTCACC
Ksp632I 2521 CTCTTC CTCTTC(1/4)
10 GAAGAG
KspI 1077 CCGCGG CCGC^GG
2052 CCGCGG
MflI 266 AGATCC [GA]^GATC[CT]
2075 AGATCC
PmaCI 775 CACGTG CAC^GTG
2659 CACGTG
PmlI 775 CACGTG CAC^GTG
2659 CACGTG
SacII 1077 CCGCGG CCGC^GG
2052 CCGCGG
TfiI 849 GATTC G^A[AT]TC
879 GAATC
XcmI 815 CCATCACCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1106 CCAAAGTGGAGGTGG
XhoII 266 AGATCC [GA]^GATC[CT]
2075 AGATCC
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# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspI AvaI
AvaII BalI BanII BbvI BcnI
BmyI BpmI BsaAI BsaHI BsaJI
BsaWI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflFI PflMI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StyI TaqI TseI Tsp45I TspRI
Tth111I TthHB8I Van91I XmaCI XmaI