Restriction map for
pFN21AE4396
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# NO CUT ENZYMES ( 113 enzymes) #
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AccI AccIII AclI AflII AflIII
AgeI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I AviII
BbrPI BclI BfrI BglI BglII
BlpI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII EagI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KspI MamI MfeI
MluI MroI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI XbaI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 82 GACGTC GACGT^C
Acc65I 764 GGTACC G^GTACC
AcsI 277 GAATTC [GA]^AATT[CT]
AhdI 843 GACCATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 246 GGGCCC GGGCC^C
ApoI 277 GAATTC [GA]^AATT[CT]
Asp718I 764 GGTACC G^GTACC
AspEI 843 GACCATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 82 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 951 CCTAGG C^CTAGG
BamHI 1227 GGATCC G^GATCC
BbsI 229 GTCTTC GAAGAC(2/6)
BcgI 566 CGAGCCAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 736 GTATCC GTATCC(6/5)
BfaI 952 CTAG C^TAG
BlnI 951 CCTAGG C^CTAGG
BpmI 470 CTGGAG CTGGAG(16/14)
BpuAI 229 GTCTTC GAAGAC(2/6)
BsgI 91 GTGCAG GTGCAG(16/14)
BsmBI 498 CGTCTC CGTCTC(1/5)
BsmI 312 GAATGC GAATGC(1/-1)
BssSI 673 CACGAG CACGAG(-5/-1)
BstYI 1227 GGATCC [GA]^GATC[CT]
DrdI 174 GACGCATGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 843 GACCATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1218 CTCTTC CTCTTC(1/4)
EcoNI 317 CCTCGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 277 GAATTC G^AATTC
EcoRV 406 GATATC GAT^ATC
KpnI 764 GGTACC GGTAC^C
Ksp632I 1218 CTCTTC CTCTTC(1/4)
MaeI 952 CTAG C^TAG
MflI 1227 GGATCC [GA]^GATC[CT]
MseI 928 TTAA T^TAA
PaeR7I 1146 CTCGAG C^TCGAG
PflFI 82 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 608 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 956 GAGCTC GAGCT^C
SapI 1217 GCTCTTC GCTCTTC(1/4)
SfcI 151 CTACAG C^T[GA][CT]AG
SphI 1158 GCATGC GCATG^C
TaqI 1147 TCGA T^CGA
Tru9I 928 TTAA T^TAA
Tth111I 82 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1147 TCGA T^CGA
Van91I 608 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1031 CCATCATCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1146 CTCGAG C^TCGAG
XhoII 1227 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
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AvaI 5 CCCGAG C^[CT]CG[GA]G
1146 CTCGAG
BalI 61 TGGCCA TGG^CCA
771 TGGCCA
BbeI 96 GGCGCC GGCGC^C
880 GGCGCC
BcnI 77 CCGGG CC^[GC]GG
112 CCCGG
BsmAI 499 GTCTC GTCTC(1/5)
1186 GTCTC
BsoBI 5 CCCGAG C^[CT]CG[GA]G
1146 CTCGAG
BstXI 266 CCAAACGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
462 CCAGCTCACTGG
HapII 77 CCGG C^CGG
113 CCGG
HpaII 77 CCGG C^CGG
113 CCGG
KasI 96 GGCGCC G^GCGCC
880 GGCGCC
MluNI 61 TGGCCA TGG^CCA
771 TGGCCA
MscI 61 TGGCCA TGG^CCA
771 TGGCCA
MspI 77 CCGG C^CGG
113 CCGG
NarI 96 GGCGCC GG^CGCC
880 GGCGCC
NciI 77 CCGGG CC^[GC]GG
112 CCCGG
PleI 127 GACTC GAGTC(4/5)
262 GAGTC
SfaNI 555 GATGC GCATC(5/9)
799 GATGC
SfoI 96 GGCGCC GGC^GCC
880 GGCGCC
SmlI 630 CTCAAG C^T[CT][GA]AG
1146 CTCGAG
TfiI 211 GAATC G^A[AT]TC
1052 GAATC
Tsp509I 278 AATT ^AATT
832 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaII BanI
BanII BbvCI BbvI BmyI BsaHI
BsaJI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HgaI HhaI
Hin1I HinP1I HinfI HphI ItaI
MaeII MaeIII MboI MboII MnlI
MslI MspA1I MvaI MvnI MwoI
NcoI NdeII NlaIII NlaIV NspI
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI StyI TseI Tsp45I TspRI