Restriction map for
pFN21AE6221
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BamHI
BbrPI BciVI BclI BfrI BglI
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HgaI HincII
HindII HindIII HpaI KpnI KspI
MaeIII MamI MluI MroI MseI
MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tsp45I Tth111I Van91I
XcmI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcsI 655 GAATTT [GA]^AATT[CT]
AcyI 167 GGCGCC G[GA]^CG[CT]C
AlwI 774 GGATC GGATC(4/5)
ApoI 655 GAATTT [GA]^AATT[CT]
BanI 167 GGCGCC G^G[CT][GA]CC
BbeI 167 GGCGCC GGCGC^C
BbsI 760 GAAGAC GAAGAC(2/6)
BglII 582 AGATCT A^GATCT
BpuAI 760 GAAGAC GAAGAC(2/6)
BsaHI 167 GGCGCC G[GA]^CG[CT]C
BseRI 314 GAGGAG GAGGAG(10/8)
BsmAI 245 GAGAC GTCTC(1/5)
BsrDI 502 CATTGC GCAATG(2/0)
BssHII 134 GCGCGC G^CGCGC
BssSI 687 CACGAG CACGAG(-5/-1)
BstF5I 552 GGATG GGATG(2/0)
BstXI 217 CCACCCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 582 AGATCT [GA]^GATC[CT]
DsaI 223 CCGTGG C^C[GA][CT]GG
EarI 694 GAAGAG CTCTTC(1/4)
EcoT14I 746 CCAAGG C^C[AT][AT]GG
FokI 552 GGATG GGATG(9/13)
Hin1I 167 GGCGCC G[GA]^CG[CT]C
HphI 811 GGTGA GGTGA(8/7)
KasI 167 GGCGCC G^GCGCC
Ksp632I 694 GAAGAG CTCTTC(1/4)
MaeII 341 ACGT A^CGT
MfeI 866 CAATTG C^AATTG
MflI 582 AGATCT [GA]^GATC[CT]
MunI 866 CAATTG C^AATTG
NarI 167 GGCGCC GG^CGCC
NlaIII 879 CATG CATG^
PaeR7I 79 CTCGAG C^TCGAG
SfaNI 6 GATGC GCATC(5/9)
SfoI 167 GGCGCC GGC^GCC
SmaI 268 CCCGGG CCC^GGG
SmlI 79 CTCGAG C^T[CT][GA]AG
StyI 746 CCAAGG C^C[AT][AT]GG
TfiI 540 GATTC G^A[AT]TC
XbaI 819 TCTAGA T^CTAGA
XhoI 79 CTCGAG C^TCGAG
XhoII 582 AGATCT [GA]^GATC[CT]
XmaCI 268 CCCGGG C^CCGGG
XmaI 268 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AfaI 19 GTAC GT^AC
792 GTAC
AluI 318 AGCT AG^CT
858 AGCT
ApaI 90 GGGCCC GGGCC^C
237 GGGCCC
BanII 90 GGGCCC G[GA]GC[CT]^C
237 GGGCCC
BbvCI 198 CCTCAGC CCTCAGC(-5/-2)
120 GCTGAGG
BbvI 381 GCAGC GCAGC(8/12)
859 GCTGC
BcgI 99 CGAGGGGACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
110 CGAGAGTCTTGC
BmyI 90 GGGCCC G[AGT]GC[ACT]^C
237 GGGCCC
Bsp1286I 90 GGGCCC G[AGT]GC[ACT]^C
237 GGGCCC
DdeI 121 CTGAG C^T[ACGT]AG
199 CTCAG
HaeII 59 GGCGCT [GA]GCGC^[CT]
167 GGCGCC
MslI 218 CACCCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
873 CACACACATG
RsaI 19 GTAC GT^AC
792 GTAC
TseI 381 GCAGC G^C[AT]GC
859 GCTGC
TspRI 204 CACTG CA[GC]TG[ACGT][ACGT]^
472 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AvaI AvaII BalI
BcnI BfaI BsaJI BsiYI BslI
BsmFI BsoBI BsrI BssKI BstNI
BstUI Cac8I CfoI Cfr13I DpnII
DraII EaeI EcoO109I EcoRII Fnu4HI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MaeI MboI MboII
MluNI MnlI MscI MspI MvaI
MvnI MwoI NciI NdeII NlaIV
PleI Sau3AI Sau96I ScrFI TaqI
Tsp509I TthHB8I