Restriction map for
pFN21AE4316
##################################
# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AvaI AviII AvrII
BbeI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DdeI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HgaI
Hin1I HpaI KasI KpnI KspI
MaeI MamI MluI MroI MseI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
PacI PaeR7I PflMI PinAI PleI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I Van91I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AcsI 853 GAATTC [GA]^AATT[CT]
AfaI 549 GTAC GT^AC
AlwNI 356 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 853 GAATTC [GA]^AATT[CT]
AspI 712 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 658 GGATCC G^GATCC
BanI 64 GGCACC G^G[CT][GA]CC
BanII 541 GGGCTC G[GA]GC[CT]^C
BbrPI 438 CACGTG CAC^GTG
BbsI 229 GAAGAC GAAGAC(2/6)
BcnI 696 CCCGG CC^[GC]GG
BpmI 517 CTGGAG CTGGAG(16/14)
BpuAI 229 GAAGAC GAAGAC(2/6)
BsaAI 438 CACGTG [CT]AC^GT[GA]
BsgI 841 GTGCAG GTGCAG(16/14)
BstBI 6 TTCGAA TT^CGAA
BstXI 110 CCACCAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 710 AGGACCC [GA]G^G[ACGT]CC[CT]
EarI 222 GAAGAG CTCTTC(1/4)
EcoNI 293 CCTTGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 710 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 853 GAATTC G^AATTC
HapII 697 CCGG C^CGG
HpaII 697 CCGG C^CGG
HphI 373 GGTGA GGTGA(8/7)
Ksp632I 222 GAAGAG CTCTTC(1/4)
MslI 486 CATCGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 514 CAGCTG C[AC]G^C[GT]G
MspI 697 CCGG C^CGG
NciI 696 CCCGG CC^[GC]GG
NcoI 676 CCATGG C^CATGG
NspV 6 TTCGAA TT^CGAA
PflFI 712 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 438 CACGTG CAC^GTG
PmlI 438 CACGTG CAC^GTG
PpuMI 710 AGGACCC [GA]G^G[AT]CC[CT]
PstI 77 CTGCAG CTGCA^G
PvuII 514 CAGCTG CAG^CTG
RsaI 549 GTAC GT^AC
SexAI 566 ACCAGGT A^CC[AT]GGT
SfuI 6 TTCGAA TT^CGAA
SmlI 544 CTCAAG C^T[CT][GA]AG
Tth111I 712 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 777 CCAGCTACAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AciI 138 CCGC CCGC(-3/-1)
507 CCGC
AspHI 650 GTGCTC G[AT]GC[AT]^C
700 GTGCTC
BbvI 513 GCAGC GCAGC(8/12)
822 GCAGC
BseRI 172 GAGGAG GAGGAG(10/8)
871 GAGGAG
BsiHKAI 650 GTGCTC G[AT]GC[AT]^C
700 GTGCTC
BsiYI 49 CCTGTCCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
293 CCTTGGAAAGG
BslI 49 CCTGTCCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
293 CCTTGGAAAGG
BspMI 177 GCAGGT ACCTGC(4/8)
843 GCAGGT
BstF5I 192 CATCC GGATG(2/0)
580 CATCC
BstYI 426 GGATCT [GA]^GATC[CT]
658 GGATCC
DsaI 449 CCGTGG C^C[GA][CT]GG
676 CCATGG
Fnu4HI 513 GCAGC GC^[ACGT]GC
822 GCAGC
FokI 192 CATCC GGATG(9/13)
580 CATCC
HincII 331 GTTGAC GT[CT]^[GA]AC
861 GTTGAC
HindII 331 GTTGAC GT[CT]^[GA]AC
861 GTTGAC
HindIII 98 AAGCTT A^AGCTT
250 AAGCTT
HinfI 4 GATTC G^A[ACGT]TC
148 GATTC
ItaI 513 GCAGC GC^[ACGT]GC
822 GCAGC
MaeII 439 ACGT A^CGT
839 ACGT
MaeIII 38 GTCAC ^GT[ACGT]AC
303 GTCAC
MfeI 122 CAATTG C^AATTG
319 CAATTG
MflI 426 GGATCT [GA]^GATC[CT]
658 GGATCC
MunI 122 CAATTG C^AATTG
319 CAATTG
NlaIII 45 CATG CATG^
677 CATG
SfaNI 466 GATGC GCATC(5/9)
827 GCATC
TaqI 7 TCGA T^CGA
270 TCGA
TfiI 4 GATTC G^A[AT]TC
148 GATTC
TseI 513 GCAGC G^C[AT]GC
822 GCAGC
Tsp45I 38 GTCAC ^GT[GC]AC
303 GTCAC
TthHB8I 7 TCGA T^CGA
270 TCGA
XhoII 426 GGATCT [GA]^GATC[CT]
658 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AluI AlwI AvaII BalI BmyI
BsaJI Bsp1286I BsrI BssKI BstNI
Cac8I CfoI Cfr13I DpnII EaeI
EcoRII EcoT14I HaeIII HhaI HinP1I
MboI MboII MluNI MnlI MscI
MvaI MwoI NdeII NlaIV Sau3AI
Sau96I ScrFI SfcI StyI Tsp509I
TspRI