Restriction map for
pFN21AE9660
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AfaI AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI ApoI AscI
Asp718I AspEI AspI AvaI AviII
AvrII BalI BbrPI BbvCI BcgI
BciVI BclI BglII BlnI BlpI
Bpu1102I BsaAI BsaI BsiEI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspHI BsrBI BsrDI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
CelII ClaI CpoI DraI DraII
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HpaI HphI
KpnI MaeII MaeIII MluI MluNI
MscI MslI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI PvuII RcaI RsaI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfiI SfuI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tsp45I TspRI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 67 enzymes) #
###################################
AccII 39 CGCG CG^CG
AccIII 46 TCCGGA T^CCGGA
AflII 697 CTTAAG C^TTAAG
AflIII 639 ACATGT A^C[GA][CT]GT
AocI 129 CCTCAGG CC^T[ACGT]AGG
AseI 414 ATTAAT AT^TAAT
AsnI 414 ATTAAT AT^TAAT
Asp700I 703 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 111 GGACC G^G[AT]CC
BamHI 687 GGATCC G^GATCC
BanI 88 GGCGCC G^G[CT][GA]CC
BanII 34 GAGCCC G[GA]GC[CT]^C
BbeI 88 GGCGCC GGCGC^C
BbsI 247 GAAGAC GAAGAC(2/6)
BcnI 60 CCGGG CC^[GC]GG
BfaI 368 CTAG C^TAG
BfrI 697 CTTAAG C^TTAAG
BpuAI 247 GAAGAC GAAGAC(2/6)
BsaBI 582 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 46 TCCGGA [AT]^CCGG[AT]
BseAI 46 TCCGGA T^CCGGA
BsmAI 318 GAGAC GTCTC(1/5)
BspEI 46 TCCGGA T^CCGGA
BspLU11I 639 ACATGT A^CATGT
BspMI 78 GCAGGT ACCTGC(4/8)
BsrFI 85 GCCGGC [GA]^CCGG[CT]
BstF5I 314 GGATG GGATG(2/0)
BstUI 39 CGCG CG^CG
BstYI 687 GGATCC [GA]^GATC[CT]
Bsu36I 129 CCTCAGG CC^T[ACGT]AGG
CfoI 89 GCGC GCG^C
Cfr10I 85 GCCGGC [GA]^CCGG[CT]
DsaI 38 CCGCGG C^C[GA][CT]GG
Eco81I 129 CCTCAGG CC^T[ACGT]AGG
FokI 314 GGATG GGATG(9/13)
HaeII 88 GGCGCC [GA]GCGC^[CT]
HgaI 25 GACGC GACGC(5/10)
HhaI 89 GCGC GCG^C
HinP1I 89 GCGC G^CGC
HincII 730 GTTGAC GT[CT]^[GA]AC
HindII 730 GTTGAC GT[CT]^[GA]AC
KasI 88 GGCGCC G^GCGCC
KspI 38 CCGCGG CCGC^GG
MaeI 368 CTAG C^TAG
MamI 582 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 675 CAATTG C^AATTG
MflI 687 GGATCC [GA]^GATC[CT]
MroI 46 TCCGGA T^CCGGA
MspA1I 38 CCGCGG C[AC]G^C[GT]G
MunI 675 CAATTG C^AATTG
MvnI 39 CGCG CG^CG
NaeI 85 GCCGGC GCC^GGC
NarI 88 GGCGCC GG^CGCC
NciI 60 CCGGG CC^[GC]GG
NgoMIV 85 GCCGGC G^CCGGC
NspI 639 ACATGT [GA]CATG^[CT]
PleI 43 GACTC GAGTC(4/5)
PshBI 414 ATTAAT AT^TAAT
PstI 712 CTGCAG CTGCA^G
SacII 38 CCGCGG CCGC^GG
SfoI 88 GGCGCC GGC^GCC
SgrAI 84 CGCCGGCG C[GA]^CCGG[CT]G
TaqI 233 TCGA T^CGA
TfiI 436 GAATC G^A[AT]TC
TthHB8I 233 TCGA T^CGA
XhoII 687 GGATCC [GA]^GATC[CT]
XmnI 703 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 25 GACGCC G[GA]^CG[CT]C
88 GGCGCC
AlwI 688 GATCC GGATC(4/5)
687 GGATC
AspHI 140 GAGCAC G[AT]GC[AT]^C
373 GTGCTC
BglI 59 GCCGGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
91 GCCCAGGCGGC
BpmI 203 CTCCAG CTGGAG(16/14)
346 CTGGAG
BsaHI 25 GACGCC G[GA]^CG[CT]C
88 GGCGCC
BsaJI 38 CCGCGG C^C[ACGT][ACGT]GG
92 CCCAGG
BseRI 127 CTCCTC GAGGAG(10/8)
4 GAGGAG
BsgI 329 CTGCAC GTGCAG(16/14)
102 GTGCAG
BsiHKAI 140 GAGCAC G[AT]GC[AT]^C
373 GTGCTC
BsiYI 603 CCTACAGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
654 CCTTATTTTGG
BslI 603 CCTACAGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
654 CCTTATTTTGG
Cfr13I 70 GGGCC G^G[ACGT]CC
111 GGACC
DpnII 588 GATC ^GATC
688 GATC
EarI 268 GAAGAG CTCTTC(1/4)
477 GAAGAG
FauI 37 CCCGC CCCGC(4/6)
68 GCGGG
HaeIII 58 GGCC GG^CC
71 GGCC
Hin1I 25 GACGCC G[GA]^CG[CT]C
88 GGCGCC
HinfI 43 GACTC G^A[ACGT]TC
436 GAATC
Ksp632I 268 GAAGAG CTCTTC(1/4)
477 GAAGAG
MboI 588 GATC ^GATC
688 GATC
NdeII 588 GATC ^GATC
688 GATC
NlaIV 88 GGCGCC GG[ACGT]^[ACGT]CC
687 GGATCC
Sau3AI 588 GATC ^GATC
688 GATC
Sau96I 70 GGGCC G^G[ACGT]CC
111 GGACC
SfaNI 18 GATGC GCATC(5/9)
184 GATGC
SfcI 604 CTACAG C^T[GA][CT]AG
712 CTGCAG
SmlI 697 CTTAAG C^T[CT][GA]AG
718 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI AluI BbvI BmyI Bsp1286I
BssKI BstNI Cac8I DdeI EcoRII
Fnu4HI HapII HindIII HpaII ItaI
MboII MnlI MseI MspI MvaI
MwoI NlaIII ScrFI Tru9I TseI
Tsp509I