Restriction map for
pFN21AE6113
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# NO CUT ENZYMES ( 184 enzymes) #
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AatII Acc65I AccI AccII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BbvCI BbvI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsiEI
BsiWI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
ClaI CpoI DraI DraII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI Fnu4HI FseI FspI HaeII
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI ItaI KasI KpnI
KspI MaeII MamI MfeI MflI
MluI MluNI MscI MspA1I MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NlaIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
TseI Tth111I TthHB8I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccIII 285 TCCGGA T^CCGGA
AcsI 115 AAATTT [GA]^AATT[CT]
ApoI 115 AAATTT [GA]^AATT[CT]
AvaII 329 GGACC G^G[AT]CC
BanII 247 GAGCTC G[GA]GC[CT]^C
BcgI 4 GCAGAGCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 164 CTGGAG CTGGAG(16/14)
BsaJI 215 CCAGGG C^C[ACGT][ACGT]GG
BsaWI 285 TCCGGA [AT]^CCGG[AT]
BseAI 285 TCCGGA T^CCGGA
BseRI 43 GAGGAG GAGGAG(10/8)
BsgI 88 CTGCAC GTGCAG(16/14)
BspEI 285 TCCGGA T^CCGGA
BstXI 113 CCAAATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I 329 GGACC G^G[ACGT]CC
DdeI 179 CTGAG C^T[ACGT]AG
DraIII 94 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 362 TGGCCG [CT]^GGCC[GA]
EarI 124 GAAGAG CTCTTC(1/4)
HapII 286 CCGG C^CGG
HgaI 172 GACGC GACGC(5/10)
HpaII 286 CCGG C^CGG
Ksp632I 124 GAAGAG CTCTTC(1/4)
MaeIII 170 GTGAC ^GT[ACGT]AC
MroI 285 TCCGGA T^CCGGA
MspI 286 CCGG C^CGG
NlaIII 390 CATG CATG^
PflMI 320 CCAGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 356 CTGCAG CTGCA^G
SacI 247 GAGCTC GAGCT^C
SapI 124 GAAGAGC GCTCTTC(1/4)
Sau96I 329 GGACC G^G[ACGT]CC
ScaI 29 AGTACT AGT^ACT
Tsp45I 170 GTGAC ^GT[GC]AC
Tsp509I 116 AATT ^AATT
TspRI 359 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 320 CCAGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 350 CCATCTCTGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AfaI 30 GTAC GT^AC
102 GTAC
AluI 162 AGCT AG^CT
248 AGCT
AlwI 84 GATCC GGATC(4/5)
342 GATCC
AspHI 72 GAGCAC G[AT]GC[AT]^C
247 GAGCTC
BfaI 40 CTAG C^TAG
314 CTAG
BsiHKAI 72 GAGCAC G[AT]GC[AT]^C
247 GAGCTC
BsiYI 320 CCAGTTGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
384 CCTATACATGG
BslI 320 CCAGTTGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
384 CCTATACATGG
BsrI 335 ACTGG ACTGG(1/-1)
320 CCAGT
DpnII 84 GATC ^GATC
342 GATC
HaeIII 21 GGCC GG^CC
363 GGCC
HinfI 48 GATTC G^A[ACGT]TC
310 GATTC
HphI 169 GGTGA GGTGA(8/7)
339 GGTGA
MaeI 40 CTAG C^TAG
314 CTAG
MboI 84 GATC ^GATC
342 GATC
MboII 124 GAAGA GAAGA(8/7)
142 GAAGA
MseI 151 TTAA T^TAA
274 TTAA
MslI 130 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
253 CATCCAGATG
MwoI 81 GCTGATCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
349 GCCATCTCTGC
NdeII 84 GATC ^GATC
342 GATC
RsaI 30 GTAC GT^AC
102 GTAC
Sau3AI 84 GATC ^GATC
342 GATC
SfaNI 211 GATGC GCATC(5/9)
129 GCATC
SfcI 206 CTACAG C^T[GA][CT]AG
356 CTGCAG
TfiI 48 GATTC G^A[AT]TC
310 GATTC
Tru9I 151 TTAA T^TAA
274 TTAA
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# 3 OR MORE CUT ENZYMES ( 10 enzymes) #
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BmyI Bsp1286I BssKI BstF5I BstNI
EcoRII FokI MnlI MvaI ScrFI