Restriction map for
pFN21AE1556
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI AlwI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BfrI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspMI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DrdI
Eam1105I Eco47III Eco81I EcoNI EcoRI
EcoRV EcoT22I FseI HgaI Hin1I
HindIII KasI KpnI KspI MaeII
MamI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
SspBI SspI SwaI XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccII 24 CGCG CG^CG
AflIII 991 ACATGT A^C[GA][CT]GT
Alw44I 516 GTGCAC G^TGCAC
ApaLI 516 GTGCAC G^TGCAC
Asp700I 972 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 586 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 852 CTCGGG C^[CT]CG[GA]G
AviII 299 TGCGCA TGC^GCA
BanII 1317 GAGCCC G[GA]GC[CT]^C
BclI 99 TGATCA T^GATCA
BfaI 949 CTAG C^TAG
BglII 754 AGATCT A^GATCT
BsgI 645 CTGCAC GTGCAG(16/14)
BsiEI 53 CGGCCG CG[GA][CT]^CG
BsmI 614 GAATGC GAATGC(1/-1)
BsoBI 852 CTCGGG C^[CT]CG[GA]G
BspHI 872 TCATGA T^CATGA
BspLU11I 991 ACATGT A^CATGT
BsrDI 1022 GCAATG GCAATG(2/0)
BstEII 1338 GGTAACC G^GT[ACGT]ACC
BstPI 1338 GGTAACC G^GT[ACGT]ACC
BstUI 24 CGCG CG^CG
BstYI 754 AGATCT [GA]^GATC[CT]
EagI 53 CGGCCG C^GGCCG
EclXI 53 CGGCCG C^GGCCG
Eco52I 53 CGGCCG C^GGCCG
EcoO65I 1338 GGTAACC G^GT[ACGT]ACC
FbaI 99 TGATCA T^GATCA
FspI 299 TGCGCA TGC^GCA
HaeII 220 AGCGCC [GA]GCGC^[CT]
HincII 370 GTTAAC GT[CT]^[GA]AC
HindII 370 GTTAAC GT[CT]^[GA]AC
HpaI 370 GTTAAC GTT^AAC
HphI 509 TCACC GGTGA(8/7)
MaeI 949 CTAG C^TAG
MflI 754 AGATCT [GA]^GATC[CT]
MvnI 24 CGCG CG^CG
NdeI 359 CATATG CA^TATG
PflFI 586 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1303 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 590 AGGTCCC [GA]G^G[AT]CC[CT]
PvuII 810 CAGCTG CAG^CTG
RcaI 872 TCATGA T^CATGA
SapI 618 GCTCTTC GCTCTTC(1/4)
SbfI 327 CCTGCAGG CCTGCA^GG
Sse8387I 327 CCTGCAGG CCTGCA^GG
StuI 461 AGGCCT AGG^CCT
TaqI 473 TCGA T^CGA
TfiI 352 GATTC G^A[AT]TC
Tth111I 586 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 473 TCGA T^CGA
Van91I 1303 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 754 AGATCT [GA]^GATC[CT]
XmnI 972 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
BglI 223 GCCATGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1113 GCCCCTGGGGC
BseRI 841 CTCCTC GAGGAG(10/8)
1298 CTCCTC
DraIII 510 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
1163 CACCGTGTG
DsaI 224 CCATGG C^C[GA][CT]GG
916 CCATGG
EaeI 53 CGGCCG [CT]^GGCC[GA]
596 CGGCCA
FauI 160 GCGGG CCCGC(4/6)
270 GCGGG
MspA1I 261 CCGCTG C[AC]G^C[GT]G
810 CAGCTG
NcoI 224 CCATGG C^CATGG
916 CCATGG
NspI 314 GCATGT [GA]CATG^[CT]
991 ACATGT
SfaNI 451 GATGC GCATC(5/9)
187 GCATC
Tsp45I 923 GTGAC ^GT[GC]AC
1036 GTGAC
XcmI 173 CCAACTTCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1280 CCACAAAATCAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI AspHI AvaII BbvI BcnI
BmyI BpmI BsaI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrBI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI Ksp632I
MaeIII MboI MboII MnlI MseI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
RsaI Sau3AI Sau96I ScrFI SfcI
StyI Tru9I TseI Tsp509I TspRI