Restriction map for
pFN21AE0500
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# NO CUT ENZYMES ( 110 enzymes) #
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Acc65I AccIII AclI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BamHI BbeI BbrPI
BcgI BciVI BclI BfrI BglI
BlnI BsaAI BsaBI BseAI BsgI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV FbaI FseI FspI HgaI
HindIII KasI KpnI KspI MamI
MfeI MroI MunI NaeI NarI
NgoMIV NotI NruI PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AatII 1138 GACGTC GACGT^C
AccI 970 GTCTAC GT^[AC][GT]AC
AcyI 1138 GACGTC G[GA]^CG[CT]C
AflIII 1412 ACGCGT A^C[GA][CT]GT
Asp700I 313 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1747 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1133 CCCGGG C^[CT]CG[GA]G
AvaII 1975 GGACC G^G[AT]CC
BanI 145 GGTGCC G^G[CT][GA]CC
BbvCI 1641 GCTGAGG CCTCAGC(-5/-2)
BlpI 1761 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1761 GCTGAGC GC^T[ACGT]AGC
BsaHI 1138 GACGTC G[GA]^CG[CT]C
BsaWI 1169 ACCGGA [AT]^CCGG[AT]
BsoBI 1133 CCCGGG C^[CT]CG[GA]G
BspMI 1232 ACCTGC ACCTGC(4/8)
BsrFI 1517 ACCGGC [GA]^CCGG[CT]
BstXI 2120 CCATGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1761 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1517 ACCGGC [GA]^CCGG[CT]
DraI 1725 TTTAAA TTT^AAA
Hin1I 1138 GACGTC G[GA]^CG[CT]C
HpaI 1315 GTTAAC GTT^AAC
HphI 1696 TCACC GGTGA(8/7)
MaeII 1139 ACGT A^CGT
MluI 1412 ACGCGT A^CGCGT
NdeI 1037 CATATG CA^TATG
NheI 2140 GCTAGC G^CTAGC
NspI 338 GCATGT [GA]CATG^[CT]
PflFI 1747 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SmaI 1133 CCCGGG CCC^GGG
Tsp45I 1195 GTGAC ^GT[GC]AC
Tth111I 1747 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1133 CCCGGG C^CCGGG
XmaI 1133 CCCGGG C^CCGGG
XmnI 313 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AfaI 871 GTAC GT^AC
997 GTAC
ApaI 1583 GGGCCC GGGCC^C
1777 GGGCCC
BglII 1208 AGATCT A^GATCT
1473 AGATCT
BsrBI 327 CCGCTC CCGCTC(-3/-3)
1404 CCGCTC
BstAPI 1278 GCAACAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1355 GCAGCGCCTGC
BstBI 320 TTCGAA TT^CGAA
957 TTCGAA
DraII 1582 AGGGCCC [GA]G^G[ACGT]CC[CT]
1583 GGGCCCT
DsaI 5 CCATGG C^C[GA][CT]GG
1124 CCATGG
EcoO109I 1582 AGGGCCC [GA]G^G[ACGT]CC[CT]
1583 GGGCCCT
EcoT22I 1103 ATGCAT ATGCA^T
1450 ATGCAT
HaeII 1357 AGCGCC [GA]GCGC^[CT]
1839 GGCGCT
HincII 286 GTCAAC GT[CT]^[GA]AC
1315 GTTAAC
HindII 286 GTCAAC GT[CT]^[GA]AC
1315 GTTAAC
MaeIII 256 GTAAC ^GT[ACGT]AC
1195 GTGAC
MspA1I 404 CAGCTG C[AC]G^C[GT]G
2125 CAGCTG
NcoI 5 CCATGG C^CATGG
1124 CCATGG
NsiI 1103 ATGCAT ATGCA^T
1450 ATGCAT
NspV 320 TTCGAA TT^CGAA
957 TTCGAA
PvuII 404 CAGCTG CAG^CTG
2125 CAGCTG
RsaI 871 GTAC GT^AC
997 GTAC
SfuI 320 TTCGAA TT^CGAA
957 TTCGAA
SmlI 1954 CTCAAG C^T[CT][GA]AG
1987 CTTGAG
StuI 451 AGGCCT AGG^CCT
2105 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
AlwNI ApoI AspHI BalI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EaeI EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI Ksp632I MaeI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XcmI XhoII