Restriction map for
pFN21AE1568
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# NO CUT ENZYMES ( 150 enzymes) #
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Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII BalI BamHI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglII BlpI Bpu1102I
BpuAI BsaBI BsaI BsaWI BseAI
BsiWI BsmBI BsmFI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DpnII
DraI DraII DraIII DrdI DsaI
Eam1105I Eco47III Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HgaI HindIII HpaI HphI
KpnI KspI MamI MboI MfeI
MflI MluI MluNI MroI MscI
MslI MspA1I MunI NciI NcoI
NdeI NdeII NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SapI Sau3AI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 354 GACGTC GACGT^C
AvrII 874 CCTAGG C^CTAGG
BbeI 419 GGCGCC GGCGC^C
BfaI 875 CTAG C^TAG
BglI 411 GCCTGAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 874 CCTAGG C^CTAGG
BpmI 292 CTGGAG CTGGAG(16/14)
BsaAI 684 CACGTA [CT]AC^GT[GA]
BseRI 28 GAGGAG GAGGAG(10/8)
BsiEI 96 CGGCCG CG[GA][CT]^CG
BsmAI 203 GAGAC GTCTC(1/5)
BsmI 787 GCATTC GAATGC(1/-1)
BsrFI 43 GCCGGC [GA]^CCGG[CT]
BssHII 101 GCGCGC G^CGCGC
BssKI 672 CCTGG ^CC[ACGT]GG
BstAPI 200 GCAGAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 672 CCTGG CC^[AT]GG
Cfr10I 43 GCCGGC [GA]^CCGG[CT]
EagI 96 CGGCCG C^GGCCG
EarI 465 GAAGAG CTCTTC(1/4)
EclXI 96 CGGCCG C^GGCCG
Eco52I 96 CGGCCG C^GGCCG
EcoRI 474 GAATTC G^AATTC
EcoRII 672 CCTGG ^CC[AT]GG
FauI 108 GCGGG CCCGC(4/6)
HaeII 419 GGCGCC [GA]GCGC^[CT]
HapII 44 CCGG C^CGG
HincII 316 GTTGAC GT[CT]^[GA]AC
HindII 316 GTTGAC GT[CT]^[GA]AC
HpaII 44 CCGG C^CGG
KasI 419 GGCGCC G^GCGCC
Ksp632I 465 GAAGAG CTCTTC(1/4)
MaeI 875 CTAG C^TAG
MseI 254 TTAA T^TAA
MspI 44 CCGG C^CGG
MvaI 672 CCTGG CC^[AT]GG
NaeI 43 GCCGGC GCC^GGC
NarI 419 GGCGCC GG^CGCC
NgoMIV 43 GCCGGC G^CCGGC
NspI 175 GCATGT [GA]CATG^[CT]
ScrFI 672 CCTGG CC^[ACGT]GG
SfoI 419 GGCGCC GGC^GCC
StuI 80 AGGCCT AGG^CCT
TfiI 676 GAATC G^A[AT]TC
Tru9I 254 TTAA T^TAA
XcmI 386 CCAGAGCTCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 10 AAATTC [GA]^AATT[CT]
474 GAATTC
AcyI 354 GACGTC G[GA]^CG[CT]C
419 GGCGCC
AlwNI 17 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
201 CAGAGACTG
ApoI 10 AAATTC [GA]^AATT[CT]
474 GAATTC
Asp700I 148 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
783 GAAGGCATTC
AvaII 714 GGTCC G^G[AT]CC
883 GGTCC
BanI 419 GGCGCC G^G[CT][GA]CC
447 GGTGCC
BsaHI 354 GACGTC G[GA]^CG[CT]C
419 GGCGCC
BsgI 747 CTGCAC GTGCAG(16/14)
887 CTGCAC
BsrDI 47 GCAATG GCAATG(2/0)
599 GCAATG
BsrI 231 ACTGG ACTGG(1/-1)
69 CCAGT
DdeI 413 CTGAG C^T[ACGT]AG
461 CTCAG
EaeI 66 CGGCCA [CT]^GGCC[GA]
96 CGGCCG
Hin1I 354 GACGTC G[GA]^CG[CT]C
419 GGCGCC
NlaIII 176 CATG CATG^
396 CATG
PleI 19 GACTC GAGTC(4/5)
400 GACTC
SacI 31 GAGCTC GAGCT^C
389 GAGCTC
Tsp45I 179 GTCAC ^GT[GC]AC
352 GTGAC
Tsp509I 11 AATT ^AATT
475 AATT
XmnI 148 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
783 GAAGGCATTC
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# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
BanII BbvI BmyI BsaJI BsiHKAI
BsiYI BslI Bsp1286I BstF5I BstUI
Cac8I CfoI Cfr13I EcoT14I Fnu4HI
FokI HaeIII HhaI HinP1I HinfI
ItaI MaeII MaeIII MboII MnlI
MvnI MwoI NlaIV PstI RsaI
Sau96I SfaNI SfcI SmlI StyI
TseI TspRI