Restriction map for
pFN21AE4523
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII BalI BbeI BbrPI
BcgI BciVI BfrI BglI BglII
BsaAI BsaBI BsaHI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BsrGI
BssHII Bst1107I BstAPI BstBI BstUI
BstZ17I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FseI FspI
Hin1I HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MscI MseI MunI MvnI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TaqI TfiI Tru9I
Tth111I TthHB8I XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 871 GTCTAC GT^[AC][GT]AC
AccIII 1414 TCCGGA T^CCGGA
AfaI 834 GTAC GT^AC
AflIII 954 ACATGT A^C[GA][CT]GT
AvaI 1046 CTCGGG C^[CT]CG[GA]G
AvrII 21 CCTAGG C^CTAGG
BbvCI 993 CCTCAGC CCTCAGC(-5/-2)
BlnI 21 CCTAGG C^CTAGG
BlpI 369 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 369 GCTGAGC GC^T[ACGT]AGC
BsaI 1051 GGTCTC GGTCTC(1/5)
BsaWI 1414 TCCGGA [AT]^CCGG[AT]
BseAI 1414 TCCGGA T^CCGGA
BseRI 218 CTCCTC GAGGAG(10/8)
BsoBI 1046 CTCGGG C^[CT]CG[GA]G
BspEI 1414 TCCGGA T^CCGGA
BspHI 1031 TCATGA T^CATGA
BspLU11I 954 ACATGT A^CATGT
BsrBI 1043 CCGCTC CCGCTC(-3/-3)
BsrDI 1238 GCAATG GCAATG(2/0)
BsrFI 887 GCCGGC [GA]^CCGG[CT]
BssSI 1114 CACGAG CACGAG(-5/-1)
BstEII 597 GGTAACC G^GT[ACGT]ACC
BstPI 597 GGTAACC G^GT[ACGT]ACC
BstXI 1524 CCAGCAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 369 GCTGAGC GC^T[ACGT]AGC
Cfr10I 887 GCCGGC [GA]^CCGG[CT]
EcoNI 1630 CCTGTAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 597 GGTAACC G^GT[ACGT]ACC
FauI 1014 GCGGG CCCGC(4/6)
HaeII 1000 AGCGCC [GA]GCGC^[CT]
HgaI 87 GACGC GACGC(5/10)
MroI 1414 TCCGGA T^CCGGA
NaeI 887 GCCGGC GCC^GGC
NcoI 1405 CCATGG C^CATGG
NgoMIV 887 GCCGGC G^CCGGC
NspI 954 ACATGT [GA]CATG^[CT]
PstI 913 CTGCAG CTGCA^G
RcaI 1031 TCATGA T^CATGA
RsaI 834 GTAC GT^AC
Tsp509I 1206 AATT ^AATT
XbaI 822 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BanI 655 GGCACC G^G[CT][GA]CC
850 GGTGCC
BbsI 1109 GAAGAC GAAGAC(2/6)
1671 GAAGAC
BclI 341 TGATCA T^GATCA
1460 TGATCA
BpuAI 1109 GAAGAC GAAGAC(2/6)
1671 GAAGAC
BspMI 1304 ACCTGC ACCTGC(4/8)
564 GCAGGT
CfoI 732 GCGC GCG^C
1001 GCGC
DraII 429 AGGACCT [GA]G^G[ACGT]CC[CT]
454 AGGTCCC
DraIII 289 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
418 CACTATGTG
EcoO109I 429 AGGACCT [GA]G^G[ACGT]CC[CT]
454 AGGTCCC
FbaI 341 TGATCA T^GATCA
1460 TGATCA
HhaI 732 GCGC GCG^C
1001 GCGC
HinP1I 732 GCGC G^CGC
1001 GCGC
HincII 1018 GTCAAC GT[CT]^[GA]AC
1210 GTTGAC
HindII 1018 GTCAAC GT[CT]^[GA]AC
1210 GTTGAC
MaeII 552 ACGT A^CGT
644 ACGT
MslI 415 CATCACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1155 CACCACAGTG
MspA1I 79 CAGCGG C[AC]G^C[GT]G
1012 CAGCGG
PpuMI 429 AGGACCT [GA]G^G[AT]CC[CT]
454 AGGTCCC
SacI 1027 GAGCTC GAGCT^C
1290 GAGCTC
SapI 133 GAAGAGC GCTCTTC(1/4)
1062 GCTCTTC
StuI 482 AGGCCT AGG^CCT
582 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AluI AlwI AlwNI AocI
AspHI AvaII BamHI BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I Cfr13I
DdeI DpnII DsaI EaeI EarI
Eco81I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TseI Tsp45I
TspRI Van91I XhoII