Restriction map for
pFN21AE6307
##################################
# NO CUT ENZYMES ( 155 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI AlwNI AocI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BbsI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaI BsaWI BseAI BseRI
BsiEI BsiWI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrFI BsrGI
BssHII Bst1107I BstBI BstEII BstF5I
BstPI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT14I
FbaI FokI FseI FspI HapII
HgaI HincII HindII HindIII HpaI
HpaII KpnI MaeI MaeIII MfeI
MluNI MroI MscI MslI MspI
MunI NaeI NciI NcoI NdeI
NgoMIV NheI NlaIII NotI NruI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmeI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SalI SbfI ScaI
SexAI SfcI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI Tsp45I Tth111I TthHB8I
XbaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AcyI 3 GGCGCC G[GA]^CG[CT]C
AfaI 143 GTAC GT^AC
AflIII 514 ACGCGT A^C[GA][CT]GT
Alw44I 155 GTGCAC G^TGCAC
ApaI 257 GGGCCC GGGCC^C
ApaLI 155 GTGCAC G^TGCAC
AspHI 155 GTGCAC G[AT]GC[AT]^C
BbeI 3 GGCGCC GGCGC^C
BbrPI 342 CACGTG CAC^GTG
BpmI 262 CTCCAG CTGGAG(16/14)
BsaAI 342 CACGTG [CT]AC^GT[GA]
BsaBI 721 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3 GGCGCC G[GA]^CG[CT]C
BsaJI 41 CCGCGG C^C[ACGT][ACGT]GG
BsgI 491 CTGCAC GTGCAG(16/14)
BsiHKAI 155 GTGCAC G[AT]GC[AT]^C
BsiYI 341 CCACGTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 341 CCACGTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsrBI 303 GAGCGG CCGCTC(-3/-3)
BssKI 730 CCTGG ^CC[ACGT]GG
BssSI 543 CACGAG CACGAG(-5/-1)
BstAPI 832 GCAGTTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 730 CCTGG CC^[AT]GG
BstXI 191 CCAAATGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 4 GCGC GCG^C
DsaI 41 CCGCGG C^C[GA][CT]GG
EaeI 101 TGGCCG [CT]^GGCC[GA]
EcoRI 772 GAATTC G^AATTC
EcoRII 730 CCTGG ^CC[AT]GG
EcoT22I 922 ATGCAT ATGCA^T
HaeII 3 GGCGCC [GA]GCGC^[CT]
HhaI 4 GCGC GCG^C
Hin1I 3 GGCGCC G[GA]^CG[CT]C
HinP1I 4 GCGC G^CGC
HinfI 667 GAATC G^A[ACGT]TC
KasI 3 GGCGCC G^GCGCC
KspI 41 CCGCGG CCGC^GG
MaeII 343 ACGT A^CGT
MamI 721 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 514 ACGCGT A^CGCGT
MspA1I 41 CCGCGG C[AC]G^C[GT]G
MvaI 730 CCTGG CC^[AT]GG
NarI 3 GGCGCC GG^CGCC
NsiI 922 ATGCAT ATGCA^T
PflMI 341 CCACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 342 CACGTG CAC^GTG
PmlI 342 CACGTG CAC^GTG
PpuMI 14 AGGTCCT [GA]G^G[AT]CC[CT]
RsaI 143 GTAC GT^AC
SacII 41 CCGCGG CCGC^GG
SapI 239 GAAGAGC GCTCTTC(1/4)
ScrFI 730 CCTGG CC^[ACGT]GG
SfaNI 921 GATGC GCATC(5/9)
SfoI 3 GGCGCC GGC^GCC
TfiI 667 GAATC G^A[AT]TC
Van91I 341 CCACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 264 CCAGAACAATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 10 enzymes) #
###################################
AvaII 15 GGTCC G^G[AT]CC
693 GGACC
BamHI 528 GGATCC G^GATCC
726 GGATCC
BanI 3 GGCGCC G^G[CT][GA]CC
334 GGCACC
BanII 257 GGGCCC G[GA]GC[CT]^C
307 GGGCTC
BbvI 64 GCAGC GCAGC(8/12)
187 GCTGC
BspMI 400 ACCTGC ACCTGC(4/8)
12 GCAGGT
EarI 239 GAAGAG CTCTTC(1/4)
873 GAAGAG
FauI 9 CCCGC CCCGC(4/6)
305 GCGGG
Ksp632I 239 GAAGAG CTCTTC(1/4)
873 GAAGAG
TseI 64 GCAGC G^C[AT]GC
187 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
ApoI BmyI Bsp1286I BsrI BstUI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I Fnu4HI HaeIII HphI
ItaI MboI MboII MflI MnlI
MseI MvnI MwoI NdeII NlaIV
Sau3AI Sau96I Tru9I Tsp509I TspRI
XhoII