Restriction map for
pFN21AB5690
##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
Acc65I AccIII AflII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AviII AvrII BamHI BbrPI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BseAI
BseRI BsiEI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrGI BssSI BstBI BstEII BstPI
CelII ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRV EcoT22I FbaI
FspI HindIII HpaI KpnI MfeI
MluI MroI MunI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI PvuI RsrII
SacI SalI SapI ScaI SexAI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI SspBI SspI StuI
SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 430 GACGTC GACGT^C
AclI 676 AACGTT AA^CGTT
AflIII 380 ACACGT A^C[GA][CT]GT
AlwI 721 GGATC GGATC(4/5)
AocI 1507 CCTGAGG CC^T[ACGT]AGG
BalI 233 TGGCCA TGG^CCA
BbeI 217 GGCGCC GGCGC^C
BbsI 478 GAAGAC GAAGAC(2/6)
BbvCI 1129 CCTCAGC CCTCAGC(-5/-2)
BpuAI 478 GAAGAC GAAGAC(2/6)
BsaAI 381 CACGTA [CT]AC^GT[GA]
BsaBI 285 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 634 GAGACC GGTCTC(1/5)
BsaWI 1481 TCCGGT [AT]^CCGG[AT]
BsgI 1298 GTGCAG GTGCAG(16/14)
BspHI 1424 TCATGA T^CATGA
BsrDI 528 CATTGC GCAATG(2/0)
BssHII 54 GCGCGC G^CGCGC
Bst1107I 792 GTATAC GTA^TAC
BstYI 721 GGATCT [GA]^GATC[CT]
BstZ17I 792 GTATAC GTA^TAC
Bsu36I 1507 CCTGAGG CC^T[ACGT]AGG
DrdI 1504 GACCCTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1507 CCTGAGG CC^T[ACGT]AGG
FseI 1158 GGCCGGCC GGCCGG^CC
KasI 217 GGCGCC G^GCGCC
MamI 285 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 721 GGATCT [GA]^GATC[CT]
MluNI 233 TGGCCA TGG^CCA
MscI 233 TGGCCA TGG^CCA
NarI 217 GGCGCC GG^CGCC
NcoI 236 CCATGG C^CATGG
PflMI 1397 CCATTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 676 AACGTT AA^CGTT
PvuII 1021 CAGCTG CAG^CTG
RcaI 1424 TCATGA T^CATGA
SbfI 249 CCTGCAGG CCTGCA^GG
SfoI 217 GGCGCC GGC^GCC
Sse8387I 249 CCTGCAGG CCTGCA^GG
Van91I 1397 CCATTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1202 CCACAAATGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 721 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 241 GTAGAC GT^[AC][GT]AC
792 GTATAC
AspI 481 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1011 GACTGGGTC
BfaI 404 CTAG C^TAG
595 CTAG
BsmAI 634 GAGAC GTCTC(1/5)
782 GAGAC
BsrBI 414 CCGCTC CCGCTC(-3/-3)
1376 CCGCTC
BsrFI 703 GCCGGC [GA]^CCGG[CT]
1159 GCCGGC
BstAPI 1300 GCAGAAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1309 GCAGGTGCTGC
BstXI 224 CCAACTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1226 CCACGGTGATGG
Cfr10I 703 GCCGGC [GA]^CCGG[CT]
1159 GCCGGC
EarI 808 GAAGAG CTCTTC(1/4)
1269 GAAGAG
EcoRI 991 GAATTC G^AATTC
1038 GAATTC
HincII 325 GTTGAC GT[CT]^[GA]AC
523 GTCAAC
HindII 325 GTTGAC GT[CT]^[GA]AC
523 GTCAAC
Ksp632I 808 GAAGAG CTCTTC(1/4)
1269 GAAGAG
MaeI 404 CTAG C^TAG
595 CTAG
MslI 687 CATGCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1227 CACGGTGATG
NaeI 703 GCCGGC GCC^GGC
1159 GCCGGC
NgoMIV 703 GCCGGC G^CCGGC
1159 GCCGGC
PflFI 481 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1011 GACTGGGTC
TaqI 644 TCGA T^CGA
1256 TCGA
Tth111I 481 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1011 GACTGGGTC
TthHB8I 644 TCGA T^CGA
1256 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwNI ApoI AvaI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaHI BsaJI BsiYI BslI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KspI
MaeII MaeIII MboI MboII MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI RsaI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XmaCI XmaI