Restriction map for
pFN21AE6212
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII AccI AclI AflII AgeI
AhdI AlwI AlwNI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BalI
BamHI BbeI BbvCI BciVI BclI
BcnI BfrI BglI BlnI BlpI
Bpu1102I BsaBI BsaI BseRI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrFI BsrGI Bst1107I
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraII DraIII
Eam1105I EarI Eco47III Eco81I EcoNI
EcoO109I EcoRV EcoT22I FbaI FseI
FspI HaeII HindIII HinfI HpaI
KasI Ksp632I MamI MfeI MluNI
MscI MseI MunI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NsiI NspI PacI PaeR7I
PflFI PflMI PinAI PleI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I TspRI Tth111I Van91I
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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Acc65I 128 GGTACC G^GTACC
AccIII 472 TCCGGA T^CCGGA
AcsI 538 GAATTC [GA]^AATT[CT]
AfaI 129 GTAC GT^AC
ApoI 538 GAATTC [GA]^AATT[CT]
Asp718I 128 GGTACC G^GTACC
BanI 128 GGTACC G^G[CT][GA]CC
BbsI 385 GTCTTC GAAGAC(2/6)
BcgI 20 CGATACTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 374 CTAG C^TAG
BglII 95 AGATCT A^GATCT
BpuAI 385 GTCTTC GAAGAC(2/6)
BsaWI 472 TCCGGA [AT]^CCGG[AT]
BseAI 472 TCCGGA T^CCGGA
BsiEI 430 CGGCCG CG[GA][CT]^CG
BsmFI 153 GGGAC GGGAC(10/14)
BspEI 472 TCCGGA T^CCGGA
BspMI 266 ACCTGC ACCTGC(4/8)
BsrI 273 CCAGT ACTGG(1/-1)
BssHII 238 GCGCGC G^CGCGC
BssSI 286 CTCGTG CACGAG(-5/-1)
BstBI 391 TTCGAA TT^CGAA
BstF5I 333 CATCC GGATG(2/0)
BstYI 95 AGATCT [GA]^GATC[CT]
DdeI 63 CTCAG C^T[ACGT]AG
DrdI 457 GACACCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 430 CGGCCG [CT]^GGCC[GA]
EagI 430 CGGCCG C^GGCCG
EclXI 430 CGGCCG C^GGCCG
Eco52I 430 CGGCCG C^GGCCG
EcoRI 538 GAATTC G^AATTC
EcoT14I 314 CCTTGG C^C[AT][AT]GG
FauI 534 GCGGG CCCGC(4/6)
FokI 333 CATCC GGATG(9/13)
HincII 3 GTTGAC GT[CT]^[GA]AC
HindII 3 GTTGAC GT[CT]^[GA]AC
KpnI 128 GGTACC GGTAC^C
KspI 584 CCGCGG CCGC^GG
MaeI 374 CTAG C^TAG
MflI 95 AGATCT [GA]^GATC[CT]
MluI 140 ACGCGT A^CGCGT
MroI 472 TCCGGA T^CCGGA
MslI 552 CACCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 85 CATATG CA^TATG
NlaIII 382 CATG CATG^
NspV 391 TTCGAA TT^CGAA
PvuII 205 CAGCTG CAG^CTG
RsaI 129 GTAC GT^AC
SacI 411 GAGCTC GAGCT^C
SacII 584 CCGCGG CCGC^GG
SfaNI 683 GCATC GCATC(5/9)
SfuI 391 TTCGAA TT^CGAA
StyI 314 CCTTGG C^C[AT][AT]GG
TaqI 392 TCGA T^CGA
Tsp509I 539 AATT ^AATT
TthHB8I 392 TCGA T^CGA
XbaI 373 TCTAGA T^CTAGA
XhoII 95 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 58 GACGCC G[GA]^CG[CT]C
640 GGCGTC
AflIII 122 ACGTGT A^C[GA][CT]GT
140 ACGCGT
Alw44I 298 GTGCAC G^TGCAC
693 GTGCAC
ApaLI 298 GTGCAC G^TGCAC
693 GTGCAC
AvaI 226 CCCGAG C^[CT]CG[GA]G
364 CCCGAG
AvaII 311 GGACC G^G[AT]CC
633 GGTCC
BbrPI 121 CACGTG CAC^GTG
166 CACGTG
BsaAI 121 CACGTG [CT]AC^GT[GA]
166 CACGTG
BsaHI 58 GACGCC G[GA]^CG[CT]C
640 GGCGTC
BsgI 559 GTGCAG GTGCAG(16/14)
568 GTGCAG
BsiYI 227 CCGAGAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
361 CCTCCCGAGGG
BslI 227 CCGAGAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
361 CCTCCCGAGGG
BsoBI 226 CCCGAG C^[CT]CG[GA]G
364 CCCGAG
BsrBI 615 CCGCTC CCGCTC(-3/-3)
307 GAGCGG
BsrDI 11 CATTGC GCAATG(2/0)
663 CATTGC
BstAPI 210 GCAGCCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
561 GCAGTATGTGC
BstEII 465 GGTCACC G^GT[ACGT]ACC
480 GGTCACC
BstPI 465 GGTCACC G^GT[ACGT]ACC
480 GGTCACC
DpnII 96 GATC ^GATC
198 GATC
DsaI 279 CCACGG C^C[GA][CT]GG
584 CCGCGG
EcoO65I 465 GGTCACC G^GT[ACGT]ACC
480 GGTCACC
Hin1I 58 GACGCC G[GA]^CG[CT]C
640 GGCGTC
MaeII 122 ACGT A^CGT
167 ACGT
MaeIII 466 GTCAC ^GT[ACGT]AC
481 GTCAC
MboI 96 GATC ^GATC
198 GATC
NdeII 96 GATC ^GATC
198 GATC
PmaCI 121 CACGTG CAC^GTG
166 CACGTG
PmlI 121 CACGTG CAC^GTG
166 CACGTG
Sau3AI 96 GATC ^GATC
198 GATC
Tsp45I 466 GTCAC ^GT[GC]AC
481 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AccII AciI AluI AspHI BanII
BbvI BmyI BpmI BsaJI BsiHKAI
Bsp1286I BssKI BstNI BstUI Cac8I
CfoI Cfr13I EcoRII Fnu4HI HaeIII
HapII HgaI HhaI HinP1I HpaII
HphI ItaI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NlaIV
PstI Sau96I ScrFI SfcI TseI