Restriction map for
pFN21AE1381
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I AspEI AviII
BalI BbrPI BbsI BcgI BciVI
BclI BfrI BglII BpuAI BsaAI
BsaBI BseAI BsiWI BsmI BspDI
BspEI BspHI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DraIII DrdI Eam1105I Eco47III
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HpaI
MamI MfeI MluI MluNI MroI
MscI MseI MslI MunI NcoI
NdeI NheI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI ScaI
SexAI SfuI SgrAI SnaBI SpeI
SspI SwaI TfiI Tru9I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 881 GGTACC G^GTACC
AccI 2098 GTCTAC GT^[AC][GT]AC
AcsI 718 AAATTC [GA]^AATT[CT]
AocI 1575 CCTGAGG CC^T[ACGT]AGG
ApoI 718 AAATTC [GA]^AATT[CT]
Asp718I 881 GGTACC G^GTACC
AspI 418 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 696 CCTAGG C^CTAGG
BamHI 1899 GGATCC G^GATCC
BbeI 1358 GGCGCC GGCGC^C
BlnI 696 CCTAGG C^CTAGG
Bsp1407I 2024 TGTACA T^GTACA
BsrFI 1361 GCCGGC [GA]^CCGG[CT]
BsrGI 2024 TGTACA T^GTACA
BssSI 274 CTCGTG CACGAG(-5/-1)
BstAPI 1907 GCAGTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1899 GGATCC [GA]^GATC[CT]
Bsu36I 1575 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1361 GCCGGC [GA]^CCGG[CT]
DpnII 1900 GATC ^GATC
DsaI 1133 CCGCGG C^C[GA][CT]GG
EarI 2017 CTCTTC CTCTTC(1/4)
Eco81I 1575 CCTGAGG CC^T[ACGT]AGG
HgaI 383 GCGTC GACGC(5/10)
HindIII 729 AAGCTT A^AGCTT
HinfI 16 GAGTC G^A[ACGT]TC
KasI 1358 GGCGCC G^GCGCC
KpnI 881 GGTACC GGTAC^C
Ksp632I 2017 CTCTTC CTCTTC(1/4)
KspI 1133 CCGCGG CCGC^GG
MboI 1900 GATC ^GATC
MflI 1899 GGATCC [GA]^GATC[CT]
NaeI 1361 GCCGGC GCC^GGC
NarI 1358 GGCGCC GG^CGCC
NdeII 1900 GATC ^GATC
NgoMIV 1361 GCCGGC G^CCGGC
NotI 229 GCGGCCGC GC^GGCCGC
PflFI 418 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 933 CCAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 16 GAGTC GAGTC(4/5)
SacII 1133 CCGCGG CCGC^GG
Sau3AI 1900 GATC ^GATC
SbfI 1545 CCTGCAGG CCTGCA^GG
SfiI 143 GGCCGCAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1358 GGCGCC GGC^GCC
SphI 314 GCATGC GCATG^C
Sse8387I 1545 CCTGCAGG CCTGCA^GG
SspBI 2024 TGTACA T^GTACA
TaqI 1951 TCGA T^CGA
Tsp509I 719 AATT ^AATT
Tth111I 418 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1951 TCGA T^CGA
Van91I 933 CCAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1899 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 382 GGCGTC G[GA]^CG[CT]C
1358 GGCGCC
AflIII 1287 ACATGT A^C[GA][CT]GT
1682 ACATGT
AlwI 1900 GATCC GGATC(4/5)
1899 GGATC
BsaHI 382 GGCGTC G[GA]^CG[CT]C
1358 GGCGCC
BsaI 754 GAGACC GGTCTC(1/5)
2122 GAGACC
BsaWI 652 ACCGGA [AT]^CCGG[AT]
1077 ACCGGA
BsiEI 151 CGGCCG CG[GA][CT]^CG
230 CGGCCG
BsmBI 348 CGTCTC CGTCTC(1/5)
384 CGTCTC
BspLU11I 1287 ACATGT A^CATGT
1682 ACATGT
BspMI 777 ACCTGC ACCTGC(4/8)
1086 GCAGGT
BsrBI 835 GAGCGG CCGCTC(-3/-3)
1081 GAGCGG
BsrDI 1304 CATTGC GCAATG(2/0)
1309 CATTGC
BstXI 1922 CCAAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2199 CCAGGGAGATGG
EagI 151 CGGCCG C^GGCCG
230 CGGCCG
EclXI 151 CGGCCG C^GGCCG
230 CGGCCG
Eco52I 151 CGGCCG C^GGCCG
230 CGGCCG
EcoT14I 389 CCTTGG C^C[AT][AT]GG
696 CCTAGG
Hin1I 382 GGCGTC G[GA]^CG[CT]C
1358 GGCGCC
MaeII 110 ACGT A^CGT
268 ACGT
MboII 671 TCTTC GAAGA(8/7)
2018 TCTTC
SmlI 632 CTCAAG C^T[CT][GA]AG
1321 CTCAAG
StuI 1550 AGGCCT AGG^CCT
1946 AGGCCT
StyI 389 CCTTGG C^C[AT][AT]GG
696 CCTAGG
XcmI 527 CCATCTCCCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1164 CCAGGTGCGGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
ApaI AspHI AvaI AvaII BanI
BanII BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
Cac8I CelII CfoI Cfr13I DdeI
DraII EaeI EcoNI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MaeI MaeIII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIII NlaIV NspI PpuMI PstI
PvuII RsaI SacI Sau96I ScrFI
SfaNI SfcI SmaI TseI Tsp45I
TspRI XmaCI XmaI