Restriction map for
pFN21AE6416
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BbvCI BclI BfrI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiHKAI BsiWI BsmBI BsmI
BsoBI BspDI BspEI BspLU11I BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII CfoI
ClaI CpoI DraIII DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT14I EcoT22I
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MunI MvnI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I StuI StyI SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 394 GTCTAC GT^[AC][GT]AC
AlwNI 980 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 374 GGGCCC GGGCC^C
Asp700I 148 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII 374 GGGCCC G[GA]GC[CT]^C
BcgI 522 CGACGTGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 162 GGATAC GTATCC(6/5)
BfaI 451 CTAG C^TAG
BglI 1036 GCCTTATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 374 GGGCCC G[AGT]GC[ACT]^C
BsaAI 244 TACGTG [CT]AC^GT[GA]
BsiEI 411 CGGTCG CG[GA][CT]^CG
BsmAI 359 GTCTC GTCTC(1/5)
Bsp1286I 374 GGGCCC G[AGT]GC[ACT]^C
Bsp1407I 1274 TGTACA T^GTACA
BspHI 461 TCATGA T^CATGA
BspMI 1102 GCAGGT ACCTGC(4/8)
BsrBI 78 GAGCGG CCGCTC(-3/-3)
BsrGI 1274 TGTACA T^GTACA
BstAPI 976 GCAGCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1413 AGATCC [GA]^GATC[CT]
DrdI 580 GACATTGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 559 GAATTC G^AATTC
HincII 603 GTTAAC GT[CT]^[GA]AC
HindII 603 GTTAAC GT[CT]^[GA]AC
HindIII 326 AAGCTT A^AGCTT
HpaI 603 GTTAAC GTT^AAC
HphI 4 TCACC GGTGA(8/7)
MaeI 451 CTAG C^TAG
MflI 1413 AGATCC [GA]^GATC[CT]
NaeI 32 GCCGGC GCC^GGC
NgoMIV 32 GCCGGC G^CCGGC
NspI 1280 GCATGT [GA]CATG^[CT]
PflMI 1324 CCACTTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1104 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 1100 CTGCAG CTGCA^G
RcaI 461 TCATGA T^CATGA
SspBI 1274 TGTACA T^GTACA
SspI 728 AATATT AAT^ATT
Van91I 1324 CCACTTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 617 CCAGGGAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1413 AGATCC [GA]^GATC[CT]
XmnI 148 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 1018 GTAC GT^AC
1275 GTAC
BcnI 132 CCGGG CC^[GC]GG
204 CCGGG
BsrDI 582 CATTGC GCAATG(2/0)
683 GCAATG
BsrFI 32 GCCGGC [GA]^CCGG[CT]
166 ACCGGC
BstXI 94 CCAGGCGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
476 CCACTCAGGTGG
Cfr10I 32 GCCGGC [GA]^CCGG[CT]
166 ACCGGC
DraI 1353 TTTAAA TTT^AAA
1594 TTTAAA
DraII 374 GGGCCCC [GA]G^G[ACGT]CC[CT]
1104 AGGTCCC
EaeI 43 CGGCCA [CT]^GGCC[GA]
566 TGGCCG
EcoO109I 374 GGGCCCC [GA]G^G[ACGT]CC[CT]
1104 AGGTCCC
MslI 477 CACTCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1619 CATCTTCGTG
MspA1I 125 CAGCGG C[AC]G^C[GT]G
665 CAGCGG
NciI 132 CCGGG CC^[GC]GG
204 CCGGG
RsaI 1018 GTAC GT^AC
1275 GTAC
SapI 75 GAAGAGC GCTCTTC(1/4)
151 GCTCTTC
TaqI 590 TCGA T^CGA
1461 TCGA
Tsp45I 3 GTCAC ^GT[GC]AC
59 GTCAC
TthHB8I 590 TCGA T^CGA
1461 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AvaII BbvI BsaJI BsiYI BslI
BsmFI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MseI MspI MvaI MwoI NdeII
NlaIII NlaIV PleI Sau3AI Sau96I
ScrFI SfaNI SfcI TfiI Tru9I
TseI Tsp509I TspRI