Restriction map for
pFN21AE5522
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AhdI AocI Aor51HI ApaI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BsaAI BsaBI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrGI
BssHII Bst1107I BstAPI BstBI BstYI
BstZ17I Bsu36I ClaI CpoI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KpnI Ksp632I MamI
MfeI MflI MluI MroI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SwaI Tth111I Van91I XbaI
XhoII XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccI 643 GTCGAC GT^[AC][GT]AC
AcsI 685 GAATTT [GA]^AATT[CT]
AfaI 285 GTAC GT^AC
AflIII 53 ACGTGT A^C[GA][CT]GT
AgeI 500 ACCGGT A^CCGGT
Alw44I 762 GTGCAC G^TGCAC
AlwI 618 GATCC GGATC(4/5)
ApaLI 762 GTGCAC G^TGCAC
ApoI 685 GAATTT [GA]^AATT[CT]
BbeI 136 GGCGCC GGCGC^C
BfaI 718 CTAG C^TAG
BglI 255 GCCCTCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 607 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 607 GCTGAGC GC^T[ACGT]AGC
BsaI 878 GAGACC GGTCTC(1/5)
BsaWI 500 ACCGGT [AT]^CCGG[AT]
BseRI 490 CTCCTC GAGGAG(10/8)
BspMI 743 ACCTGC ACCTGC(4/8)
BsrFI 500 ACCGGT [GA]^CCGG[CT]
BssSI 895 CTCGTG CACGAG(-5/-1)
BstEII 84 GGTGACC G^GT[ACGT]ACC
BstPI 84 GGTGACC G^GT[ACGT]ACC
BstXI 5 CCAGTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 607 GCTGAGC GC^T[ACGT]AGC
Cfr10I 500 ACCGGT [GA]^CCGG[CT]
DpnII 618 GATC ^GATC
DraI 421 TTTAAA TTT^AAA
EcoO65I 84 GGTGACC G^GT[ACGT]ACC
HaeII 136 GGCGCC [GA]GCGC^[CT]
HincII 643 GTCGAC GT[CT]^[GA]AC
HindII 643 GTCGAC GT[CT]^[GA]AC
KasI 136 GGCGCC G^GCGCC
KspI 1041 CCGCGG CCGC^GG
MaeI 718 CTAG C^TAG
MboI 618 GATC ^GATC
MseI 422 TTAA T^TAA
NarI 136 GGCGCC GG^CGCC
NcoI 167 CCATGG C^CATGG
NdeII 618 GATC ^GATC
PaeR7I 196 CTCGAG C^TCGAG
PinAI 500 ACCGGT A^CCGGT
PleI 646 GACTC GAGTC(4/5)
PpuMI 731 AGGACCT [GA]G^G[AT]CC[CT]
RsaI 285 GTAC GT^AC
SacII 1041 CCGCGG CCGC^GG
SalI 643 GTCGAC G^TCGAC
Sau3AI 618 GATC ^GATC
SfaNI 383 GCATC GCATC(5/9)
SfoI 136 GGCGCC GGC^GCC
SmaI 827 CCCGGG CCC^GGG
SphI 210 GCATGC GCATG^C
SspI 472 AATATT AAT^ATT
StuI 302 AGGCCT AGG^CCT
Tru9I 422 TTAA T^TAA
Tsp509I 686 AATT ^AATT
XhoI 196 CTCGAG C^TCGAG
XmaCI 827 CCCGGG C^CCGGG
XmaI 827 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 93 CGCG CG^CG
1042 CGCG
AcyI 136 GGCGCC G[GA]^CG[CT]C
787 GGCGTC
AlwNI 574 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG
974 CAGCGTCTG
BalI 506 TGGCCA TGG^CCA
934 TGGCCA
BpmI 691 CTCCAG CTGGAG(16/14)
124 CTGGAG
BsaHI 136 GGCGCC G[GA]^CG[CT]C
787 GGCGTC
BsgI 229 CTGCAC GTGCAG(16/14)
745 CTGCAC
BspHI 614 TCATGA T^CATGA
1065 TCATGA
BsrBI 921 CCGCTC CCGCTC(-3/-3)
199 GAGCGG
BstF5I 360 GGATG GGATG(2/0)
657 GGATG
BstUI 93 CGCG CG^CG
1042 CGCG
Cac8I 210 GCATGC GC[ACGT]^[ACGT]GC
264 GCAGGC
DraII 731 AGGACCT [GA]G^G[ACGT]CC[CT]
1010 AGGCCCT
EcoO109I 731 AGGACCT [GA]G^G[ACGT]CC[CT]
1010 AGGCCCT
EcoT14I 167 CCATGG C^C[AT][AT]GG
633 CCTTGG
FauI 105 GCGGG CCCGC(4/6)
453 GCGGG
FokI 360 GGATG GGATG(9/13)
657 GGATG
Hin1I 136 GGCGCC G[GA]^CG[CT]C
787 GGCGTC
MluNI 506 TGGCCA TGG^CCA
934 TGGCCA
MscI 506 TGGCCA TGG^CCA
934 TGGCCA
MslI 18 CATCTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
663 CATGGACATG
MspA1I 146 CAGCGG C[AC]G^C[GT]G
1041 CCGCGG
MvnI 93 CGCG CG^CG
1042 CGCG
NspI 191 ACATGC [GA]CATG^[CT]
210 GCATGC
PstI 154 CTGCAG CTGCA^G
571 CTGCAG
RcaI 614 TCATGA T^CATGA
1065 TCATGA
SacI 610 GAGCTC GAGCT^C
706 GAGCTC
SfcI 154 CTGCAG C^T[GA][CT]AG
571 CTGCAG
StyI 167 CCATGG C^C[AT][AT]GG
633 CCTTGG
Tsp45I 85 GTGAC ^GT[GC]AC
590 GTGAC
XcmI 347 CCAAGAAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
984 CCACTCCCTCGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AluI AspHI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BmyI BpuAI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstNI CfoI Cfr13I
DdeI DsaI EaeI EcoRII Fnu4HI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboII MnlI MspI MvaI
MwoI NciI NlaIII NlaIV Sau96I
ScrFI SmlI TaqI TfiI TseI
TspRI TthHB8I