Restriction map for
pFN21AE1380
##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AvrII BamHI BbeI
BbrPI BciVI BfrI BglI BlnI
BsaBI BsaWI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I ClaI CpoI DraI
DraIII Eam1105I Eco47III EcoRI EcoRV
EcoT22I HaeII HindIII HpaI KasI
KpnI MamI MfeI MroI MseI
MunI NarI NcoI NdeI NheI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SspBI
SspI SwaI Tru9I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AlwNI 328 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 1668 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 1751 GCAAAGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1259 TGATCA T^GATCA
BfaI 137 CTAG C^TAG
BlpI 478 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 478 GCTGAGC GC^T[ACGT]AGC
BsaAI 772 TACGTG [CT]AC^GT[GA]
BsmBI 1446 CGTCTC CGTCTC(1/5)
BsrDI 1265 GCAATG GCAATG(2/0)
BstAPI 1946 GCACCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 478 GCTGAGC GC^T[ACGT]AGC
EarI 199 CTCTTC CTCTTC(1/4)
FbaI 1259 TGATCA T^GATCA
FseI 636 GGCCGGCC GGCCGG^CC
HincII 397 GTCAAC GT[CT]^[GA]AC
HindII 397 GTCAAC GT[CT]^[GA]AC
Ksp632I 199 CTCTTC CTCTTC(1/4)
KspI 6 CCGCGG CCGC^GG
MaeI 137 CTAG C^TAG
MaeII 773 ACGT A^CGT
MluI 1849 ACGCGT A^CGCGT
MslI 1239 CATGCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 637 GCCGGC GCC^GGC
NgoMIV 637 GCCGGC G^CCGGC
NotI 588 GCGGCCGC GC^GGCCGC
PaeR7I 580 CTCGAG C^TCGAG
PflFI 1668 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 240 GACTC GAGTC(4/5)
SacI 1293 GAGCTC GAGCT^C
SacII 6 CCGCGG CCGC^GG
SbfI 756 CCTGCAGG CCTGCA^GG
Sse8387I 756 CCTGCAGG CCTGCA^GG
TfiI 338 GAATC G^A[AT]TC
Tsp509I 361 AATT ^AATT
Tth111I 1668 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 580 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AcyI 790 GACGCC G[GA]^CG[CT]C
1096 GACGCC
AflIII 773 ACGTGT A^C[GA][CT]GT
1849 ACGCGT
Alw44I 1143 GTGCAC G^TGCAC
1318 GTGCAC
AocI 660 CCTGAGG CC^T[ACGT]AGG
1495 CCTGAGG
ApaLI 1143 GTGCAC G^TGCAC
1318 GTGCAC
BalI 41 TGGCCA TGG^CCA
956 TGGCCA
BbsI 313 GTCTTC GAAGAC(2/6)
1702 GTCTTC
BglII 809 AGATCT A^GATCT
839 AGATCT
BpuAI 313 GTCTTC GAAGAC(2/6)
1702 GTCTTC
BsaHI 790 GACGCC G[GA]^CG[CT]C
1096 GACGCC
BsaI 144 GGTCTC GGTCTC(1/5)
1673 GGTCTC
BsiEI 589 CGGCCG CG[GA][CT]^CG
683 CGGCCG
BspMI 1242 GCAGGT ACCTGC(4/8)
1425 GCAGGT
BsrFI 637 GCCGGC [GA]^CCGG[CT]
1630 ACCGGC
BstEII 372 GGTCACC G^GT[ACGT]ACC
1576 GGTGACC
BstPI 372 GGTCACC G^GT[ACGT]ACC
1576 GGTGACC
Bsu36I 660 CCTGAGG CC^T[ACGT]AGG
1495 CCTGAGG
Cfr10I 637 GCCGGC [GA]^CCGG[CT]
1630 ACCGGC
DrdI 1201 GACAAGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1681 GACCAGGAAGTC
EagI 589 CGGCCG C^GGCCG
683 CGGCCG
EclXI 589 CGGCCG C^GGCCG
683 CGGCCG
Eco52I 589 CGGCCG C^GGCCG
683 CGGCCG
Eco81I 660 CCTGAGG CC^T[ACGT]AGG
1495 CCTGAGG
EcoNI 897 CCTGGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1495 CCTGAGGGAGG
EcoO65I 372 GGTCACC G^GT[ACGT]ACC
1576 GGTGACC
Hin1I 790 GACGCC G[GA]^CG[CT]C
1096 GACGCC
HinfI 240 GACTC G^A[ACGT]TC
338 GAATC
MluNI 41 TGGCCA TGG^CCA
956 TGGCCA
MscI 41 TGGCCA TGG^CCA
956 TGGCCA
PflMI 327 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
561 CCAGGACATGG
SmaI 390 CCCGGG CCC^GGG
730 CCCGGG
SphI 545 GCATGC GCATG^C
1238 GCATGC
Van91I 327 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
561 CCAGGACATGG
XcmI 557 CCAGCCAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1958 CCACCAGCAGCATGG
XmaCI 390 CCCGGG C^CCGGG
730 CCCGGG
XmaI 390 CCCGGG C^CCGGG
730 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
ApaI AspHI AvaI AvaII AviII
BanI BanII BbvCI BbvI BcnI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeIII
HapII HgaI HhaI HinP1I HpaII
HphI ItaI MaeIII MboI MboII
MflI MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TseI Tsp45I TspRI TthHB8I XhoII