Restriction map for
pFN21AE1450
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# NO CUT ENZYMES ( 159 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspHI
AspI AvaI AviII AvrII BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BmyI
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmFI BsoBI Bsp1286I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoT14I EcoT22I FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HindIII HpaII KasI
KpnI KspI MamI MluI MroI
MspI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StyI
SwaI TaqI Tth111I TthHB8I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AlwNI 1058 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 183 ATTAAT AT^TAAT
AsnI 183 ATTAAT AT^TAAT
Asp700I 418 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 15 GGACC G^G[AT]CC
BalI 477 TGGCCA TGG^CCA
BlpI 1077 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1077 GCTCAGC GC^T[ACGT]AGC
BsaJI 1062 CCCTGG C^C[ACGT][ACGT]GG
BsgI 1067 GTGCAG GTGCAG(16/14)
BsmBI 654 GAGACG CGTCTC(1/5)
Bsp1407I 84 TGTACA T^GTACA
BspMI 17 ACCTGC ACCTGC(4/8)
BsrDI 640 GCAATG GCAATG(2/0)
BsrGI 84 TGTACA T^GTACA
BssSI 1168 CACGAG CACGAG(-5/-1)
BstAPI 1085 GCAGACTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I 1261 GCTGGC GC[ACGT]^[ACGT]GC
CelII 1077 GCTCAGC GC^T[ACGT]AGC
Cfr13I 15 GGACC G^G[ACGT]CC
EaeI 477 TGGCCA [CT]^GGCC[GA]
EcoRI 422 GAATTC G^AATTC
EcoRV 544 GATATC GAT^ATC
FauI 343 GCGGG CCCGC(4/6)
HincII 606 GTTAAC GT[CT]^[GA]AC
HindII 606 GTTAAC GT[CT]^[GA]AC
HpaI 606 GTTAAC GTT^AAC
HphI 1150 GGTGA GGTGA(8/7)
MaeII 751 ACGT A^CGT
MfeI 41 CAATTG C^AATTG
MluNI 477 TGGCCA TGG^CCA
MscI 477 TGGCCA TGG^CCA
MslI 955 CATAGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 11 CAGCGG C[AC]G^C[GT]G
MunI 41 CAATTG C^AATTG
NspI 493 ACATGC [GA]CATG^[CT]
PflMI 1057 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 656 GACGAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 183 ATTAAT AT^TAAT
PstI 691 CTGCAG CTGCA^G
Sau96I 15 GGACC G^G[ACGT]CC
SmlI 883 CTTGAG C^T[CT][GA]AG
SspBI 84 TGTACA T^GTACA
SspI 1003 AATATT AAT^ATT
Tsp45I 1151 GTGAC ^GT[GC]AC
Van91I 1057 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 418 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AcsI 422 GAATTC [GA]^AATT[CT]
1137 GAATTT
AlwI 628 GATCC GGATC(4/5)
1198 GGATC
ApoI 422 GAATTC [GA]^AATT[CT]
1137 GAATTT
BfaI 379 CTAG C^TAG
832 CTAG
BsmI 740 GAATGC GAATGC(1/-1)
398 GCATTC
BsrI 152 ACTGG ACTGG(1/-1)
450 CCAGT
BstYI 627 AGATCC [GA]^GATC[CT]
1198 GGATCT
DpnII 628 GATC ^GATC
1199 GATC
EarI 96 GAAGAG CTCTTC(1/4)
990 GAAGAG
HinfI 156 GAATC G^A[ACGT]TC
507 GATTC
Ksp632I 96 GAAGAG CTCTTC(1/4)
990 GAAGAG
MaeI 379 CTAG C^TAG
832 CTAG
MaeIII 1151 GTGAC ^GT[ACGT]AC
1248 GTTAC
MboI 628 GATC ^GATC
1199 GATC
MflI 627 AGATCC [GA]^GATC[CT]
1198 GGATCT
NdeII 628 GATC ^GATC
1199 GATC
Sau3AI 628 GATC ^GATC
1199 GATC
SfcI 581 CTACAG C^T[GA][CT]AG
691 CTGCAG
StuI 729 AGGCCT AGG^CCT
1097 AGGCCT
TfiI 156 GAATC G^A[AT]TC
507 GATTC
TspRI 511 CACTG CA[GC]TG[ACGT][ACGT]^
636 CAGTG
XhoII 627 AGATCC [GA]^GATC[CT]
1198 GGATCT
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AfaI AluI BbvI BpmI
BseRI BsiYI BslI BsmAI BssKI
BstF5I BstNI DdeI EcoRII Fnu4HI
FokI HaeIII ItaI MboII MnlI
MseI MvaI MwoI NlaIII RsaI
ScrFI SfaNI Tru9I TseI Tsp509I