Restriction map for
pFN21AE6410
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# NO CUT ENZYMES ( 136 enzymes) #
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AatII Acc65I AccI AccII AclI
AflII AflIII AgeI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspHI AviII BalI BamHI BanII
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglII BlpI BmyI
Bpu1102I BpuAI BsaAI BsaBI BseRI
BsiEI BsiHKAI BsiWI BsmBI Bsp1286I
Bsp1407I BspDI BspLU11I BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DpnII DraI DraIII
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI FbaI FseI
FspI HindIII KpnI KspI MamI
MboI MfeI MflI MluI MluNI
MscI MunI MvnI NaeI NdeI
NdeII NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI Sau3AI SbfI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccIII 634 TCCGGA T^CCGGA
AcsI 862 AAATTT [GA]^AATT[CT]
AhdI 1127 GACAGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 862 AAATTT [GA]^AATT[CT]
AspEI 1127 GACAGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1247 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1076 CTCGGG C^[CT]CG[GA]G
AvrII 576 CCTAGG C^CTAGG
BbeI 85 GGCGCC GGCGC^C
BcnI 8 CCCGG CC^[GC]GG
BglI 131 GCCTGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 576 CCTAGG C^CTAGG
BsaI 975 GGTCTC GGTCTC(1/5)
BsaWI 634 TCCGGA [AT]^CCGG[AT]
BseAI 634 TCCGGA T^CCGGA
BsmI 350 GAATGC GAATGC(1/-1)
BsoBI 1076 CTCGGG C^[CT]CG[GA]G
BspEI 634 TCCGGA T^CCGGA
BspHI 180 TCATGA T^CATGA
BsrBI 781 CCGCTC CCGCTC(-3/-3)
DraII 377 AGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 146 CCATGG C^C[GA][CT]GG
EaeI 545 TGGCCG [CT]^GGCC[GA]
Eam1105I 1127 GACAGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1265 CTCTTC CTCTTC(1/4)
EcoNI 68 CCTGGCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 377 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRV 1153 GATATC GAT^ATC
EcoT22I 445 ATGCAT ATGCA^T
HpaI 877 GTTAAC GTT^AAC
KasI 85 GGCGCC G^GCGCC
Ksp632I 1265 CTCTTC CTCTTC(1/4)
MroI 634 TCCGGA T^CCGGA
MseI 878 TTAA T^TAA
NarI 85 GGCGCC GG^CGCC
NciI 8 CCCGG CC^[GC]GG
NcoI 146 CCATGG C^CATGG
NsiI 445 ATGCAT ATGCA^T
PflFI 1247 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 554 CCAGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 940 CTGCAG CTGCA^G
RcaI 180 TCATGA T^CATGA
SapI 1264 GCTCTTC GCTCTTC(1/4)
ScaI 222 AGTACT AGT^ACT
SfoI 85 GGCGCC GGC^GCC
SmlI 795 CTTGAG C^T[CT][GA]AG
SphI 447 GCATGC GCATG^C
TfiI 656 GAATC G^A[AT]TC
Tru9I 878 TTAA T^TAA
Tth111I 1247 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 554 CCAGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcyI 85 GGCGCC G[GA]^CG[CT]C
364 GACGCC
BfaI 577 CTAG C^TAG
841 CTAG
BsaHI 85 GGCGCC G[GA]^CG[CT]C
364 GACGCC
BsgI 38 GTGCAG GTGCAG(16/14)
617 GTGCAG
BsmAI 976 GTCTC GTCTC(1/5)
1365 GTCTC
HgaI 364 GACGC GACGC(5/10)
1251 GCGTC
Hin1I 85 GGCGCC G[GA]^CG[CT]C
364 GACGCC
HincII 877 GTTAAC GT[CT]^[GA]AC
1135 GTCAAC
HindII 877 GTTAAC GT[CT]^[GA]AC
1135 GTCAAC
HphI 580 GGTGA GGTGA(8/7)
1173 TCACC
MaeI 577 CTAG C^TAG
841 CTAG
MboII 753 TCTTC GAAGA(8/7)
1266 TCTTC
MslI 175 CACTTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
823 CATGAACATG
PleI 550 GACTC GAGTC(4/5)
1109 GAGTC
PvuII 926 CAGCTG CAG^CTG
988 CAGCTG
StuI 129 AGGCCT AGG^CCT
1072 AGGCCT
TaqI 203 TCGA T^CGA
920 TCGA
TthHB8I 203 TCGA T^CGA
920 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AfaI AluI AvaII BanI
BbvI BpmI BsaJI BsiYI BslI
BsmFI BspMI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MaeII MaeIII
MnlI MspA1I MspI MvaI MwoI
NlaIII NlaIV NspI RsaI Sau96I
ScrFI SfaNI SfcI StyI TseI
Tsp45I Tsp509I TspRI