Restriction map for
pFN21AE1053
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI ApoI AscI AseI AsnI
Asp718I AspEI AspI BalI BamHI
BbrPI BbsI BciVI BclI BfrI
BglII BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI Bst1107I
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
Eam1105I EarI EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HindIII
HpaI KpnI Ksp632I MamI MfeI
MluI MluNI MroI MscI MseI
MunI NdeI NheI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsaI RsrII SalI
SapI SbfI ScaI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tru9I
Tsp509I Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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Alw44I 1114 GTGCAC G^TGCAC
AlwI 171 GGATC GGATC(4/5)
Aor51HI 653 AGCGCT AGC^GCT
ApaLI 1114 GTGCAC G^TGCAC
Asp700I 1044 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 1028 GGACC G^G[AT]CC
AvrII 9 CCTAGG C^CTAGG
BbvCI 94 CCTCAGC CCTCAGC(-5/-2)
BfaI 10 CTAG C^TAG
BlnI 9 CCTAGG C^CTAGG
BseRI 312 GAGGAG GAGGAG(10/8)
BssHII 527 GCGCGC G^CGCGC
BstXI 384 CCAGCCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 171 GGATCT [GA]^GATC[CT]
Eco47III 653 AGCGCT AGC^GCT
HgaI 435 GACGC GACGC(5/10)
HincII 499 GTCAAC GT[CT]^[GA]AC
HindII 499 GTCAAC GT[CT]^[GA]AC
MaeI 10 CTAG C^TAG
MaeII 182 ACGT A^CGT
MaeIII 433 GTGAC ^GT[ACGT]AC
MboII 1013 TCTTC GAAGA(8/7)
MflI 171 GGATCT [GA]^GATC[CT]
NcoI 108 CCATGG C^CATGG
PstI 888 CTGCAG CTGCA^G
SexAI 842 ACCTGGT A^CC[AT]GGT
SfcI 888 CTGCAG C^T[GA][CT]AG
SfiI 724 GGCCAGTGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TaqI 932 TCGA T^CGA
TfiI 358 GAATC G^A[AT]TC
Tsp45I 433 GTGAC ^GT[GC]AC
TthHB8I 932 TCGA T^CGA
XhoII 171 GGATCT [GA]^GATC[CT]
XmnI 1044 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 421 GGCGCC G[GA]^CG[CT]C
1060 GGCGCC
AocI 142 CCTAAGG CC^T[ACGT]AGG
309 CCTGAGG
ApaI 329 GGGCCC GGGCC^C
460 GGGCCC
AvaI 463 CCCGAG C^[CT]CG[GA]G
475 CCCGAG
AviII 299 TGCGCA TGC^GCA
773 TGCGCA
BbeI 421 GGCGCC GGCGC^C
1060 GGCGCC
BcgI 477 CGAGCGGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1070 GCAAGGCCTTCG
BcnI 64 CCGGG CC^[GC]GG
332 CCCGG
BpmI 135 CTCCAG CTGGAG(16/14)
915 CTGGAG
BsaHI 421 GGCGCC G[GA]^CG[CT]C
1060 GGCGCC
BsmAI 206 GAGAC GTCTC(1/5)
53 GTCTC
BsoBI 463 CCCGAG C^[CT]CG[GA]G
475 CCCGAG
BspMI 770 ACCTGC ACCTGC(4/8)
1022 ACCTGC
BstAPI 765 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
890 GCAGCCAGTGC
Bsu36I 142 CCTAAGG CC^T[ACGT]AGG
309 CCTGAGG
DpnII 172 GATC ^GATC
198 GATC
DraII 269 AGGCCCC [GA]G^G[ACGT]CC[CT]
328 AGGGCCC
Eco81I 142 CCTAAGG CC^T[ACGT]AGG
309 CCTGAGG
EcoO109I 269 AGGCCCC [GA]G^G[ACGT]CC[CT]
328 AGGGCCC
EcoT14I 9 CCTAGG C^C[AT][AT]GG
108 CCATGG
FspI 299 TGCGCA TGC^GCA
773 TGCGCA
Hin1I 421 GGCGCC G[GA]^CG[CT]C
1060 GGCGCC
KasI 421 GGCGCC G^GCGCC
1060 GGCGCC
MboI 172 GATC ^GATC
198 GATC
NarI 421 GGCGCC GG^CGCC
1060 GGCGCC
NciI 64 CCGGG CC^[GC]GG
332 CCCGG
NdeII 172 GATC ^GATC
198 GATC
NlaIII 109 CATG CATG^
121 CATG
NotI 731 GCGGCCGC GC^GGCCGC
998 GCGGCCGC
Sau3AI 172 GATC ^GATC
198 GATC
SfoI 421 GGCGCC GGC^GCC
1060 GGCGCC
StyI 9 CCTAGG C^C[AT][AT]GG
108 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AluI AlwNI AspHI
BanI BanII BbvI BglI BmyI
BsaJI BsgI BsiEI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BsrBI BsrFI
BsrI BssKI BssSI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DsaI EaeI EagI EclXI
Eco52I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI KspI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NgoMIV NlaIV
PleI SacI SacII Sau96I ScrFI
SfaNI StuI TseI TspRI