Restriction map for
pFN21AE4549
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AccIII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI AspEI AspI AvrII BbeI
BbrPI BbsI BclI BlnI BlpI
Bpu1102I BpuAI BsaI BsaWI BseAI
BsiWI BsmBI BsmI BspEI BspLU11I
BsrBI BsrDI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII CpoI DraI DraIII Eam1105I
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HindIII HpaI
KasI MfeI MluI MroI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI PvuI RsrII SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tth111I XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 930 GACGTC GACGT^C
Acc65I 188 GGTACC G^GTACC
AccI 409 GTCGAC GT^[AC][GT]AC
AclI 1258 AACGTT AA^CGTT
AcsI 1761 GAATTT [GA]^AATT[CT]
AflII 838 CTTAAG C^TTAAG
ApaI 478 GGGCCC GGGCC^C
ApoI 1761 GAATTT [GA]^AATT[CT]
Asp718I 188 GGTACC G^GTACC
AspHI 1750 GAGCTC G[AT]GC[AT]^C
AviII 344 TGCGCA TGC^GCA
BbvCI 360 GCTGAGG CCTCAGC(-5/-2)
BfrI 838 CTTAAG C^TTAAG
BglII 740 AGATCT A^GATCT
BsaAI 1738 TACGTG [CT]AC^GT[GA]
BsaBI 1392 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 178 GAGGAG GAGGAG(10/8)
BsiHKAI 1750 GAGCTC G[AT]GC[AT]^C
BsmAI 1836 GAGAC GTCTC(1/5)
Bsp1407I 659 TGTACA T^GTACA
BspHI 1775 TCATGA T^CATGA
BsrFI 191 ACCGGC [GA]^CCGG[CT]
BsrGI 659 TGTACA T^GTACA
BssHII 56 GCGCGC G^CGCGC
Cfr10I 191 ACCGGC [GA]^CCGG[CT]
EagI 196 CGGCCG C^GGCCG
EarI 1675 GAAGAG CTCTTC(1/4)
EclXI 196 CGGCCG C^GGCCG
Eco52I 196 CGGCCG C^GGCCG
EcoNI 465 CCTGGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 344 TGCGCA TGC^GCA
KpnI 188 GGTACC GGTAC^C
Ksp632I 1675 GAAGAG CTCTTC(1/4)
KspI 1322 CCGCGG CCGC^GG
MamI 1392 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 839 TTAA T^TAA
NotI 195 GCGGCCGC GC^GGCCGC
PflMI 389 CCATCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 208 GAGTC GAGTC(4/5)
PshAI 265 GACTGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1258 AACGTT AA^CGTT
RcaI 1775 TCATGA T^CATGA
SacI 1750 GAGCTC GAGCT^C
SacII 1322 CCGCGG CCGC^GG
SalI 409 GTCGAC G^TCGAC
SmaI 5 CCCGGG CCC^GGG
SmlI 838 CTTAAG C^T[CT][GA]AG
SspBI 659 TGTACA T^GTACA
Tru9I 839 TTAA T^TAA
Van91I 389 CCATCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 5 CCCGGG C^CCGGG
XmaI 5 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwNI 337 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
855 CAGCCTCTG
AvaI 5 CCCGGG C^[CT]CG[GA]G
34 CTCGGG
BalI 1343 TGGCCA TGG^CCA
1562 TGGCCA
BamHI 974 GGATCC G^GATCC
1653 GGATCC
BcgI 882 CGAACGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1793 CGACGAGAGTGC
BciVI 582 GTATCC GTATCC(6/5)
1902 GTATCC
BglI 22 GCCTGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
486 GCCCTCCCGGC
BsiEI 196 CGGCCG CG[GA][CT]^CG
1325 CGGTCG
BsoBI 5 CCCGGG C^[CT]CG[GA]G
34 CTCGGG
BspDI 682 ATCGAT AT^CGAT
1210 ATCGAT
BstXI 701 CCACCAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1409 CCAAATCCTTGG
ClaI 682 ATCGAT AT^CGAT
1210 ATCGAT
DdeI 361 CTGAG C^T[ACGT]AG
1911 CTCAG
DrdI 688 GACATCACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1779 GACTGAACAGTC
EcoT14I 587 CCAAGG C^C[AT][AT]GG
1415 CCTTGG
HaeII 30 GGCGCT [GA]GCGC^[CT]
1236 GGCGCT
HincII 409 GTCGAC GT[CT]^[GA]AC
1042 GTCAAC
HindII 409 GTCGAC GT[CT]^[GA]AC
1042 GTCAAC
MluNI 1343 TGGCCA TGG^CCA
1562 TGGCCA
MscI 1343 TGGCCA TGG^CCA
1562 TGGCCA
NspI 227 ACATGC [GA]CATG^[CT]
1277 ACATGC
PpuMI 338 AGGACCT [GA]G^G[AT]CC[CT]
823 GGGACCC
SexAI 464 ACCTGGT A^CC[AT]GGT
1897 ACCAGGT
StyI 587 CCAAGG C^C[AT][AT]GG
1415 CCTTGG
Tsp509I 733 AATT ^AATT
1762 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI Asp700I AvaII BanI BanII
BbvI BcnI BfaI BmyI BpmI
BsaHI BsaJI BsgI BsiYI BslI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DpnII DraII DsaI
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TfiI TseI Tsp45I
TspRI TthHB8I XhoII XmnI