Restriction map for
pFN21AE4575
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# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI AocI Aor51HI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BanI BbeI BcgI BciVI BclI
BfrI BlnI BsaBI BseAI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspMI BsrGI BssHII
BstAPI Bsu36I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRV EcoT22I
FbaI FseI FspI HaeII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI NarI NdeI
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AcyI 1982 GACGCC G[GA]^CG[CT]C
AflIII 1796 ACATGT A^C[GA][CT]GT
BbvCI 1009 CCTCAGC CCTCAGC(-5/-2)
BglI 1253 GCCGCACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 1982 GACGCC G[GA]^CG[CT]C
BspLU11I 1796 ACATGT A^CATGT
BsrFI 2012 GCCGGC [GA]^CCGG[CT]
BssSI 1451 CTCGTG CACGAG(-5/-1)
Bst1107I 1767 GTATAC GTA^TAC
BstBI 2108 TTCGAA TT^CGAA
BstXI 331 CCACTTGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1767 GTATAC GTA^TAC
Cfr10I 2012 GCCGGC [GA]^CCGG[CT]
DrdI 126 GACAGATTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 867 GAATTC G^AATTC
EcoT14I 683 CCATGG C^C[AT][AT]GG
HgaI 1982 GACGC GACGC(5/10)
Hin1I 1982 GACGCC G[GA]^CG[CT]C
HincII 1810 GTCAAC GT[CT]^[GA]AC
HindII 1810 GTCAAC GT[CT]^[GA]AC
HindIII 186 AAGCTT A^AGCTT
NaeI 2012 GCCGGC GCC^GGC
NcoI 683 CCATGG C^CATGG
NgoMIV 2012 GCCGGC G^CCGGC
NspV 2108 TTCGAA TT^CGAA
PflMI 1077 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 708 GCTCTTC GCTCTTC(1/4)
SfuI 2108 TTCGAA TT^CGAA
SgrAI 2011 CGCCGGCG C[GA]^CCGG[CT]G
SspI 689 AATATT AAT^ATT
StyI 683 CCATGG C^C[AT][AT]GG
Van91I 1077 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 359 CCATCATGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 35 enzymes) #
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AccI 352 GTCTAC GT^[AC][GT]AC
1767 GTATAC
AccII 21 CGCG CG^CG
23 CGCG
AcsI 867 GAATTC [GA]^AATT[CT]
1623 GAATTT
Alw44I 763 GTGCAC G^TGCAC
1207 GTGCAC
AlwNI 1660 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1915 CAGCCTCTG
ApaI 79 GGGCCC GGGCC^C
1944 GGGCCC
ApaLI 763 GTGCAC G^TGCAC
1207 GTGCAC
ApoI 867 GAATTC [GA]^AATT[CT]
1623 GAATTT
BbrPI 580 CACGTG CAC^GTG
1228 CACGTG
BbsI 1636 GAAGAC GAAGAC(2/6)
1588 GTCTTC
BglII 617 AGATCT A^GATCT
1391 AGATCT
BlpI 188 GCTTAGC GC^T[ACGT]AGC
252 GCTCAGC
Bpu1102I 188 GCTTAGC GC^T[ACGT]AGC
252 GCTCAGC
BpuAI 1636 GAAGAC GAAGAC(2/6)
1588 GTCTTC
BsaAI 580 CACGTG [CT]AC^GT[GA]
1228 CACGTG
BsaI 2175 GAGACC GGTCTC(1/5)
1410 GGTCTC
BsaWI 266 ACCGGA [AT]^CCGG[AT]
404 ACCGGA
BseRI 739 GAGGAG GAGGAG(10/8)
1038 GAGGAG
BsgI 1612 CTGCAC GTGCAG(16/14)
1465 GTGCAG
BsmI 558 GAATGC GAATGC(1/-1)
573 GAATGC
BsrBI 1215 CCGCTC CCGCTC(-3/-3)
244 GAGCGG
BsrDI 636 CATTGC GCAATG(2/0)
1593 CATTGC
BstEII 153 GGTGACC G^GT[ACGT]ACC
1478 GGTCACC
BstPI 153 GGTGACC G^GT[ACGT]ACC
1478 GGTCACC
BstUI 21 CGCG CG^CG
23 CGCG
CelII 188 GCTTAGC GC^T[ACGT]AGC
252 GCTCAGC
DsaI 609 CCGTGG C^C[GA][CT]GG
683 CCATGG
EcoO65I 153 GGTGACC G^GT[ACGT]ACC
1478 GGTCACC
MseI 1277 TTAA T^TAA
1861 TTAA
MvnI 21 CGCG CG^CG
23 CGCG
NspI 1325 ACATGC [GA]CATG^[CT]
1796 ACATGT
PmaCI 580 CACGTG CAC^GTG
1228 CACGTG
PmlI 580 CACGTG CAC^GTG
1228 CACGTG
PstI 1666 CTGCAG CTGCA^G
1912 CTGCAG
Tru9I 1277 TTAA T^TAA
1861 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BalI BanII BbvI BcnI
BfaI BmyI BpmI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII