Restriction map for
pFN21AE1417
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII AccI AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
AocI Aor51HI ApoI AscI AseI
AsnI AspEI AspI AviII AvrII
BalI BbeI BbrPI BcgI BciVI
BfrI BglI BlnI BsaAI BsaBI
BsaHI BsaI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I ClaI CpoI
DraI DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
Hin1I HindIII KasI KspI MamI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI Sse8387I SspBI StuI SwaI
Tsp45I Tsp509I Tth111I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 34 enzymes) #
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Acc65I 434 GGTACC G^GTACC
Alw44I 421 GTGCAC G^TGCAC
ApaI 1112 GGGCCC GGGCC^C
ApaLI 421 GTGCAC G^TGCAC
Asp700I 297 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 434 GGTACC G^GTACC
BclI 90 TGATCA T^GATCA
BglII 341 AGATCT A^GATCT
BlpI 1599 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1599 GCTGAGC GC^T[ACGT]AGC
BsaWI 509 ACCGGA [AT]^CCGG[AT]
BsrDI 1535 GCAATG GCAATG(2/0)
BsrFI 1437 GCCGGT [GA]^CCGG[CT]
BstAPI 1014 GCAGATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1599 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1437 GCCGGT [GA]^CCGG[CT]
EcoNI 1290 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 90 TGATCA T^GATCA
HaeII 1160 AGCGCC [GA]GCGC^[CT]
HincII 699 GTTAAC GT[CT]^[GA]AC
HindII 699 GTTAAC GT[CT]^[GA]AC
HpaI 699 GTTAAC GTT^AAC
KpnI 434 GGTACC GGTAC^C
MaeII 816 ACGT A^CGT
MaeIII 25 GTTAC ^GT[ACGT]AC
MslI 897 CATCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 32 CCATGG C^CATGG
NspI 525 GCATGC [GA]CATG^[CT]
SmaI 926 CCCGGG CCC^GGG
SphI 525 GCATGC GCATG^C
SspI 676 AATATT AAT^ATT
XmaCI 926 CCCGGG C^CCGGG
XmaI 926 CCCGGG C^CCGGG
XmnI 297 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AlwNI 586 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
1588 CAGCTGCTG
AvaI 926 CCCGGG C^[CT]CG[GA]G
1622 CCCGAG
BamHI 512 GGATCC G^GATCC
836 GGATCC
BanI 434 GGTACC G^G[CT][GA]CC
789 GGCACC
BbsI 1068 GAAGAC GAAGAC(2/6)
1371 GAAGAC
BbvCI 438 CCTCAGC CCTCAGC(-5/-2)
268 GCTGAGG
BfaI 1165 CTAG C^TAG
1511 CTAG
BpuAI 1068 GAAGAC GAAGAC(2/6)
1371 GAAGAC
BsmAI 358 GAGAC GTCTC(1/5)
1212 GAGAC
BsoBI 926 CCCGGG C^[CT]CG[GA]G
1622 CCCGAG
BsrBI 448 GAGCGG CCGCTC(-3/-3)
955 GAGCGG
DraIII 142 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
1390 CACCTGGTG
EarI 1294 GAAGAG CTCTTC(1/4)
1327 GAAGAG
HgaI 1596 GACGC GACGC(5/10)
1698 GACGC
Ksp632I 1294 GAAGAG CTCTTC(1/4)
1327 GAAGAG
MaeI 1165 CTAG C^TAG
1511 CTAG
MseI 700 TTAA T^TAA
770 TTAA
PflMI 141 CCACTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
706 CCAAAGATTGG
SacI 274 GAGCTC GAGCT^C
1090 GAGCTC
SexAI 1364 ACCTGGT A^CC[AT]GGT
1391 ACCTGGT
Tru9I 700 TTAA T^TAA
770 TTAA
Van91I 141 CCACTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
706 CCAAAGATTGG
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# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaII BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MboI MboII MflI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI TseI TspRI TthHB8I
XhoII