Restriction map for
pFN21AE6420
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
Acc65I AclI AcsI AflII AgeI
AhdI Alw44I ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AvrII BbsI BcgI BciVI BclI
BfaI BfrI BglII BlnI BpuAI
BsaBI BseRI BsgI BsiWI BsmI
BspDI BspLU11I BsrBI BsrDI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DraIII Eam1105I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KpnI KspI
MaeI MaeIII MamI MfeI MluI
MseI MunI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmeI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI SwaI TfiI
Tru9I Tsp45I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AatII 721 GACGTC GACGT^C
AccIII 764 TCCGGA T^CCGGA
AocI 1298 CCTCAGG CC^T[ACGT]AGG
Aor51HI 200 AGCGCT AGC^GCT
AspI 757 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 384 TGCGCA TGC^GCA
BbeI 603 GGCGCC GGCGC^C
BbrPI 811 CACGTG CAC^GTG
BbvCI 793 GCTGAGG CCTCAGC(-5/-2)
BsaI 1453 GAGACC GGTCTC(1/5)
BsaWI 764 TCCGGA [AT]^CCGG[AT]
BseAI 764 TCCGGA T^CCGGA
BsmBI 1029 CGTCTC CGTCTC(1/5)
Bsp1407I 1073 TGTACA T^GTACA
BspEI 764 TCCGGA T^CCGGA
BspHI 617 TCATGA T^CATGA
BsrGI 1073 TGTACA T^GTACA
Bsu36I 1298 CCTCAGG CC^T[ACGT]AGG
DrdI 721 GACGTCGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 200 AGCGCT AGC^GCT
Eco81I 1298 CCTCAGG CC^T[ACGT]AGG
FspI 384 TGCGCA TGC^GCA
KasI 603 GGCGCC G^GCGCC
MroI 764 TCCGGA T^CCGGA
NarI 603 GGCGCC GG^CGCC
NcoI 995 CCATGG C^CATGG
PflFI 757 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 811 CACGTG CAC^GTG
PmlI 811 CACGTG CAC^GTG
PshAI 717 GACGGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 617 TCATGA T^CATGA
SfoI 603 GGCGCC GGC^GCC
SspBI 1073 TGTACA T^GTACA
Tsp509I 145 AATT ^AATT
Tth111I 757 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1055 CCAACCTGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 31 enzymes) #
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AccI 169 GTCTAC GT^[AC][GT]AC
886 GTCTAC
AcyI 603 GGCGCC G[GA]^CG[CT]C
721 GACGTC
AfaI 465 GTAC GT^AC
1074 GTAC
AflIII 467 ACGTGT A^C[GA][CT]GT
812 ACGTGT
AlwNI 856 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1153 CAGCCCCTG
ApaI 27 GGGCCC GGGCC^C
803 GGGCCC
BamHI 210 GGATCC G^GATCC
538 GGATCC
BglI 595 GCCGGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
688 GCCCTGCGGGC
BlpI 327 GCTGAGC GC^T[ACGT]AGC
630 GCTCAGC
Bpu1102I 327 GCTGAGC GC^T[ACGT]AGC
630 GCTCAGC
BsaAI 466 TACGTG [CT]AC^GT[GA]
811 CACGTG
BsaHI 603 GGCGCC G[GA]^CG[CT]C
721 GACGTC
BsiEI 278 CGGCCG CG[GA][CT]^CG
359 CGGCCG
BspMI 218 ACCTGC ACCTGC(4/8)
890 ACCTGC
BsrFI 361 GCCGGC [GA]^CCGG[CT]
818 GCCGGC
BsrI 734 ACTGG ACTGG(1/-1)
1360 CCAGT
BstXI 824 CCATCACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1409 CCATCTGCTTGG
CelII 327 GCTGAGC GC^T[ACGT]AGC
630 GCTCAGC
Cfr10I 361 GCCGGC [GA]^CCGG[CT]
818 GCCGGC
EagI 278 CGGCCG C^GGCCG
359 CGGCCG
EclXI 278 CGGCCG C^GGCCG
359 CGGCCG
Eco52I 278 CGGCCG C^GGCCG
359 CGGCCG
HgaI 417 GACGC GACGC(5/10)
940 GACGC
Hin1I 603 GGCGCC G[GA]^CG[CT]C
721 GACGTC
NaeI 361 GCCGGC GCC^GGC
818 GCCGGC
NgoMIV 361 GCCGGC G^CCGGC
818 GCCGGC
PflMI 519 CCAGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1244 CCATCACCTGG
RsaI 465 GTAC GT^AC
1074 GTAC
SapI 1023 GCTCTTC GCTCTTC(1/4)
1209 GCTCTTC
StuI 285 AGGCCT AGG^CCT
1093 AGGCCT
Van91I 519 CCAGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1244 CCATCACCTGG
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# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AluI AlwI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BmyI BpmI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TseI TspRI TthHB8I XhoII