Restriction map for
pFN21AE1430
##################################
# NO CUT ENZYMES ( 157 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
AlwI AocI Aor51HI ApaI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BclI
BfaI BfrI BglII BlnI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstYI BstZ17I Bsu36I ClaI
CpoI DpnII DraI DraIII DrdI
DsaI Eam1105I EarI Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
Hin1I HincII HindII HpaI KasI
KpnI Ksp632I KspI MaeI MaeII
MamI MboI MfeI MflI MluI
MluNI MroI MscI MslI MspA1I
MunI NarI NcoI NdeI NdeII
NheI NlaIII NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SapI
Sau3AI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tth111I TthHB8I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AcsI 284 AAATTC [GA]^AATT[CT]
AfaI 801 GTAC GT^AC
Alw44I 114 GTGCAC G^TGCAC
AlwNI 88 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 114 GTGCAC G^TGCAC
ApoI 284 AAATTC [GA]^AATT[CT]
AvaII 187 GGACC G^G[AT]CC
BanI 102 GGCACC G^G[CT][GA]CC
BcgI 353 CGAAATTAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 660 GGATAC GTATCC(6/5)
BsgI 220 CTGCAC GTGCAG(16/14)
BsmAI 233 GAGAC GTCTC(1/5)
BsmFI 343 GGGAC GGGAC(10/14)
BspMI 148 GCAGGT ACCTGC(4/8)
BsrBI 157 GAGCGG CCGCTC(-3/-3)
BssHII 144 GCGCGC G^CGCGC
BstAPI 87 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 497 CCAATTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 539 AGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 163 CGGCCG [CT]^GGCC[GA]
EagI 163 CGGCCG C^GGCCG
EclXI 163 CGGCCG C^GGCCG
Eco52I 163 CGGCCG C^GGCCG
EcoO109I 539 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 689 CCTTGG C^C[AT][AT]GG
FauI 70 CCCGC CCCGC(4/6)
HindIII 239 AAGCTT A^AGCTT
HphI 433 GGTGA GGTGA(8/7)
PflMI 711 CCACCTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 646 GAGTC GAGTC(4/5)
PstI 94 CTGCAG CTGCA^G
RsaI 801 GTAC GT^AC
SacI 772 GAGCTC GAGCT^C
SfaNI 413 GCATC GCATC(5/9)
SfcI 94 CTGCAG C^T[GA][CT]AG
SmlI 823 CTCAAG C^T[CT][GA]AG
StyI 689 CCTTGG C^C[AT][AT]GG
TfiI 685 GATTC G^A[AT]TC
TspRI 639 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 711 CCACCTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
BanII 36 GGGCTC G[GA]GC[CT]^C
772 GAGCTC
BbsI 586 GAAGAC GAAGAC(2/6)
817 GAAGAC
BglI 78 GCCTTTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
123 GCCCTTCGGGC
BlpI 135 GCTCAGC GC^T[ACGT]AGC
774 GCTCAGC
BpmI 317 CTGGAG CTGGAG(16/14)
787 CTGGAG
Bpu1102I 135 GCTCAGC GC^T[ACGT]AGC
774 GCTCAGC
BpuAI 586 GAAGAC GAAGAC(2/6)
817 GAAGAC
BseRI 39 CTCCTC GAGGAG(10/8)
436 GAGGAG
BsiEI 163 CGGCCG CG[GA][CT]^CG
732 CGACCG
BsrFI 49 GCCGGC [GA]^CCGG[CT]
99 GCCGGC
BsrI 640 ACTGG ACTGG(1/-1)
695 ACTGG
BstF5I 414 CATCC GGATG(2/0)
570 GGATG
BstNI 786 CCTGG CC^[AT]GG
795 CCAGG
CelII 135 GCTCAGC GC^T[ACGT]AGC
774 GCTCAGC
Cfr10I 49 GCCGGC [GA]^CCGG[CT]
99 GCCGGC
EcoRII 786 CCTGG ^CC[AT]GG
795 CCAGG
FokI 414 CATCC GGATG(9/13)
570 GGATG
HinfI 646 GAGTC G^A[ACGT]TC
685 GATTC
MaeIII 391 GTCAC ^GT[ACGT]AC
602 GTGAC
MseI 269 TTAA T^TAA
404 TTAA
MvaI 786 CCTGG CC^[AT]GG
795 CCAGG
NaeI 49 GCCGGC GCC^GGC
99 GCCGGC
NgoMIV 49 GCCGGC G^CCGGC
99 GCCGGC
Tru9I 269 TTAA T^TAA
404 TTAA
Tsp45I 391 GTCAC ^GT[GC]AC
602 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AccII AciI AluI AspHI BbvI
BcnI BmyI BsaJI BsiHKAI BsiYI
BslI Bsp1286I BssKI BstUI Cac8I
CfoI Cfr13I DdeI Fnu4HI HaeIII
HapII HhaI HinP1I HpaII ItaI
MboII MnlI MspI MvnI MwoI
NciI NlaIV Sau96I ScrFI TseI
Tsp509I