Restriction map for
pFN21AE1545
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# NO CUT ENZYMES ( 156 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BbvI BcgI BfrI BglI BglII
BlnI BlpI BmyI BpmI Bpu1102I
BsaAI BsaBI BsaHI BseAI BsgI
BsiEI BsiHKAI BsiWI BsiYI BslI
BsmBI BsmFI BsmI Bsp1286I Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstEII BstNI BstPI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraII DrdI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoRV
EcoT14I FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I MaeII MamI
MfeI MluI MluNI MroI MscI
MunI MvaI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SnaBI Sse8387I
SspBI SspI StuI StyI SwaI
TseI Tth111I Van91I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AseI 754 ATTAAT AT^TAAT
AsnI 754 ATTAAT AT^TAAT
AvaI 478 CCCGGG C^[CT]CG[GA]G
BbsI 1138 GAAGAC GAAGAC(2/6)
BciVI 783 GGATAC GTATCC(6/5)
BclI 980 TGATCA T^GATCA
BfaI 313 CTAG C^TAG
BpuAI 1138 GAAGAC GAAGAC(2/6)
BsaI 301 GAGACC GGTCTC(1/5)
BsaWI 956 TCCGGT [AT]^CCGG[AT]
BseRI 1189 GAGGAG GAGGAG(10/8)
BsoBI 478 CCCGGG C^[CT]CG[GA]G
BstAPI 435 GCAAAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 849 GCGC GCG^C
DraI 117 TTTAAA TTT^AAA
DraIII 788 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT22I 877 ATGCAT ATGCA^T
FauI 1004 GCGGG CCCGC(4/6)
FbaI 980 TGATCA T^GATCA
Fnu4HI 38 GCGGC GC^[ACGT]GC
HhaI 849 GCGC GCG^C
HinP1I 849 GCGC G^CGC
ItaI 38 GCGGC GC^[ACGT]GC
KspI 14 CCGCGG CCGC^GG
MaeI 313 CTAG C^TAG
MslI 1200 CATGAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 14 CCGCGG C[AC]G^C[GT]G
NsiI 877 ATGCAT ATGCA^T
NspI 879 GCATGC [GA]CATG^[CT]
PleI 124 GAGTC GAGTC(4/5)
PshBI 754 ATTAAT AT^TAAT
PstI 442 CTGCAG CTGCA^G
SacII 14 CCGCGG CCGC^GG
SmaI 478 CCCGGG CCC^GGG
SmlI 224 CTCAAG C^T[CT][GA]AG
SpeI 312 ACTAGT A^CTAGT
SphI 879 GCATGC GCATG^C
XcmI 326 CCACCAGAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 478 CCCGGG C^CCGGG
XmaI 478 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccII 15 CGCG CG^CG
1003 CGCG
AluI 186 AGCT AG^CT
228 AGCT
AlwNI 236 CAGGTACTG CAG[ACGT][ACGT][ACGT]^CTG
896 CAGTTTCTG
AvaII 54 GGACC G^G[AT]CC
921 GGACC
BcnI 478 CCCGG CC^[GC]GG
479 CCGGG
BspHI 683 TCATGA T^CATGA
944 TCATGA
BssKI 478 CCCGG ^CC[ACGT]GG
479 CCGGG
BstBI 422 TTCGAA TT^CGAA
698 TTCGAA
BstF5I 270 CATCC GGATG(2/0)
546 GGATG
BstUI 15 CGCG CG^CG
1003 CGCG
BstXI 329 CCAGAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1199 CCATGAATGTGG
BstYI 347 AGATCC [GA]^GATC[CT]
690 AGATCC
DdeI 230 CTCAG C^T[ACGT]AG
869 CTGAG
DsaI 14 CCGCGG C^C[GA][CT]GG
570 CCGTGG
EcoRI 701 GAATTC G^AATTC
952 GAATTC
FokI 270 CATCC GGATG(9/13)
546 GGATG
HaeIII 40 GGCC GG^CC
321 GGCC
HapII 479 CCGG C^CGG
957 CCGG
HpaII 479 CCGG C^CGG
957 CCGG
HphI 708 GGTGA GGTGA(8/7)
913 GGTGA
MflI 347 AGATCC [GA]^GATC[CT]
690 AGATCC
MspI 479 CCGG C^CGG
957 CCGG
MvnI 15 CGCG CG^CG
1003 CGCG
MwoI 253 GCCACATTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
435 GCAAAAACTGC
NciI 478 CCCGG CC^[GC]GG
479 CCGGG
NspV 422 TTCGAA TT^CGAA
698 TTCGAA
RcaI 683 TCATGA T^CATGA
944 TCATGA
ScrFI 478 CCCGG CC^[ACGT]GG
479 CCGGG
SfcI 442 CTGCAG C^T[GA][CT]AG
1181 CTACAG
SfuI 422 TTCGAA TT^CGAA
698 TTCGAA
TfiI 737 GAATC G^A[AT]TC
1149 GATTC
TspRI 809 CACTG CA[GC]TG[ACGT][ACGT]^
1184 CAGTG
XhoII 347 AGATCC [GA]^GATC[CT]
690 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AcsI AfaI AlwI ApoI
BsaJI BsmAI BsrI Cac8I Cfr13I
DpnII HinfI MaeIII MboI MboII
MnlI MseI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I SfaNI TaqI
Tru9I Tsp45I Tsp509I TthHB8I