Restriction map for
pFN21AE5218
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII AccI AccII AccIII AciI
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspHI AspI
AviII AvrII BalI BamHI BanII
BbeI BbrPI BbsI BbvCI BcgI
BcnI BfrI BglI BglII BlnI
BmyI BpuAI BsaAI BsaBI BsaHI
BsaI BseAI BsiEI BsiHKAI BsiWI
BsmBI Bsp1286I Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrGI BsrI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
CfoI ClaI CpoI DraI DraII
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRI EcoT22I FauI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KspI MaeII MamI MfeI
MflI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NciI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tth111I
TthHB8I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 473 GGTACC G^GTACC
AlwI 3 GGATC GGATC(4/5)
AlwNI 376 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 473 GGTACC G^GTACC
AvaI 266 CCCGAG C^[CT]CG[GA]G
AvaII 328 GGACC G^G[AT]CC
BanI 473 GGTACC G^G[CT][GA]CC
BciVI 511 GGATAC GTATCC(6/5)
BclI 185 TGATCA T^GATCA
BfaI 235 CTAG C^TAG
BlpI 381 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 381 GCTGAGC GC^T[ACGT]AGC
BsaWI 175 TCCGGT [AT]^CCGG[AT]
BseRI 104 GAGGAG GAGGAG(10/8)
BsgI 412 CTGCAC GTGCAG(16/14)
BsmFI 322 GTCCC GGGAC(10/14)
BsmI 299 GCATTC GAATGC(1/-1)
BsoBI 266 CCCGAG C^[CT]CG[GA]G
BspHI 458 TCATGA T^CATGA
BsrFI 502 GCCGGT [GA]^CCGG[CT]
BstXI 341 CCATCCTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 275 GCCTGC GC[ACGT]^[ACGT]GC
CelII 381 GCTGAGC GC^T[ACGT]AGC
Cfr10I 502 GCCGGT [GA]^CCGG[CT]
DdeI 382 CTGAG C^T[ACGT]AG
DsaI 96 CCATGG C^C[GA][CT]GG
EarI 520 CTCTTC CTCTTC(1/4)
EcoNI 319 CCTGTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 550 GATATC GAT^ATC
EcoT14I 96 CCATGG C^C[AT][AT]GG
FbaI 185 TGATCA T^GATCA
HaeIII 501 GGCC GG^CC
KpnI 473 GGTACC GGTAC^C
Ksp632I 520 CTCTTC CTCTTC(1/4)
MaeI 235 CTAG C^TAG
MaeIII 288 GTCAC ^GT[ACGT]AC
MseI 152 TTAA T^TAA
NcoI 96 CCATGG C^CATGG
NdeI 16 CATATG CA^TATG
PflMI 12 CCACCATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 458 TCATGA T^CATGA
SmlI 256 CTCAAG C^T[CT][GA]AG
StyI 96 CCATGG C^C[AT][AT]GG
Tru9I 152 TTAA T^TAA
Tsp45I 288 GTCAC ^GT[GC]AC
Tsp509I 310 AATT ^AATT
Van91I 12 CCACCATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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BpmI 197 CTGGAG CTGGAG(16/14)
209 CTGGAG
BsaJI 96 CCATGG C^C[ACGT][ACGT]GG
324 CCCAGG
BsmAI 107 GAGAC GTCTC(1/5)
352 GAGAC
BssKI 325 CCAGG ^CC[ACGT]GG
423 CCAGG
BstAPI 372 GCAACAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
402 GCAAACAGTGC
BstNI 325 CCAGG CC^[AT]GG
423 CCAGG
Cfr13I 328 GGACC G^G[ACGT]CC
500 GGGCC
DpnII 4 GATC ^GATC
186 GATC
EcoRII 325 CCAGG ^CC[AT]GG
423 CCAGG
HapII 176 CCGG C^CGG
503 CCGG
HinfI 37 GACTC G^A[ACGT]TC
254 GACTC
HpaII 176 CCGG C^CGG
503 CCGG
MboI 4 GATC ^GATC
186 GATC
MspA1I 194 CAGCTG C[AC]G^C[GT]G
525 CAGCTG
MspI 176 CCGG C^CGG
503 CCGG
MvaI 325 CCAGG CC^[AT]GG
423 CCAGG
NdeII 4 GATC ^GATC
186 GATC
PleI 37 GACTC GAGTC(4/5)
254 GACTC
PvuII 194 CAGCTG CAG^CTG
525 CAGCTG
Sau3AI 4 GATC ^GATC
186 GATC
Sau96I 328 GGACC G^G[ACGT]CC
500 GGGCC
ScrFI 325 CCAGG CC^[ACGT]GG
423 CCAGG
SfaNI 126 GATGC GCATC(5/9)
378 GATGC
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AfaI AluI BbvI BsiYI BslI
BsrDI BstF5I Fnu4HI FokI HphI
ItaI MboII MnlI MslI MwoI
NlaIII NlaIV RsaI TseI TspRI