Restriction map for
pFN21AE3621
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# NO CUT ENZYMES ( 194 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvaII AviII AvrII BalI
BamHI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaJI BsaWI BseAI
BseRI BsiEI BsiWI BsiYI BslI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrFI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstYI
BstZ17I Bsu36I Cac8I CelII CfoI
Cfr10I ClaI CpoI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoT14I
EcoT22I FbaI FseI FspI HaeII
HapII HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII HphI
KasI KpnI Ksp632I KspI MamI
MboII MfeI MflI MluI MluNI
MroI MscI MspA1I MspI MunI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tsp45I
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AfaI 21 GTAC GT^AC
AflIII 563 ACATGT A^C[GA][CT]GT
AspHI 123 GAGCAC G[AT]GC[AT]^C
BbvI 487 GCTGC GCAGC(8/12)
BfaI 466 CTAG C^TAG
BsgI 628 GTGCAG GTGCAG(16/14)
BsiHKAI 123 GAGCAC G[AT]GC[AT]^C
BsmFI 194 GGGAC GGGAC(10/14)
BspLU11I 563 ACATGT A^CATGT
BsrDI 276 CATTGC GCAATG(2/0)
BssKI 159 CCAGG ^CC[ACGT]GG
BstF5I 156 CATCC GGATG(2/0)
BstNI 159 CCAGG CC^[AT]GG
BstXI 159 CCAGGTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I 462 GGCCC G^G[ACGT]CC
DraI 34 TTTAAA TTT^AAA
EcoRII 159 CCAGG ^CC[AT]GG
EcoRV 215 GATATC GAT^ATC
FauI 8 CCCGC CCCGC(4/6)
Fnu4HI 487 GCTGC GC^[ACGT]GC
FokI 156 CATCC GGATG(9/13)
HaeIII 462 GGCC GG^CC
HgaI 551 GACGC GACGC(5/10)
ItaI 487 GCTGC GC^[ACGT]GC
MaeI 466 CTAG C^TAG
MaeII 384 ACGT A^CGT
MaeIII 344 GTAAC ^GT[ACGT]AC
MnlI 30 CCTC CCTC(7/6)
MvaI 159 CCAGG CC^[AT]GG
NdeI 293 CATATG CA^TATG
NspI 563 ACATGT [GA]CATG^[CT]
PleI 469 GACTC GAGTC(4/5)
RsaI 21 GTAC GT^AC
Sau96I 462 GGCCC G^G[ACGT]CC
ScrFI 159 CCAGG CC^[ACGT]GG
SfaNI 155 GCATC GCATC(5/9)
TfiI 55 GATTC G^A[AT]TC
TseI 487 GCTGC G^C[AT]GC
Tsp509I 501 AATT ^AATT
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# TWO CUT ENZYMES ( 17 enzymes) #
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AccII 10 CGCG CG^CG
553 CGCG
AciI 9 CCGC CCGC(-3/-1)
519 CCGC
BanI 4 GGCACC G^G[CT][GA]CC
256 GGTGCC
BmyI 3 GGGCAC G[AGT]GC[ACT]^C
123 GAGCAC
BsmAI 84 GTCTC GTCTC(1/5)
567 GTCTC
Bsp1286I 3 GGGCAC G[AGT]GC[ACT]^C
123 GAGCAC
BstUI 10 CGCG CG^CG
553 CGCG
DpnII 331 GATC ^GATC
379 GATC
HinfI 55 GATTC G^A[ACGT]TC
469 GACTC
MboI 331 GATC ^GATC
379 GATC
MslI 185 CACAGATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
357 CATGCTTGTG
MvnI 10 CGCG CG^CG
553 CGCG
MwoI 250 GCTGTAGGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
621 GCCAAAGGTGC
NdeII 331 GATC ^GATC
379 GATC
Sau3AI 331 GATC ^GATC
379 GATC
SfcI 251 CTGTAG C^T[GA][CT]AG
404 CTACAG
TspRI 609 CACTG CA[GC]TG[ACGT][ACGT]^
631 CAGTG
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# 3 OR MORE CUT ENZYMES ( 8 enzymes) #
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AluI DdeI MseI NlaIII NlaIV
TaqI Tru9I TthHB8I