Restriction map for
pFN21AE1523
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# NO CUT ENZYMES ( 167 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BbvCI BbvI
BcgI BciVI BclI BfrI BglI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BseAI BseRI BsgI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRV EcoT22I FauI FbaI Fnu4HI
FseI FspI HaeII HhaI Hin1I
HinP1I HincII HindII HpaI ItaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MspA1I
MunI MvnI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI StuI SwaI TseI
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AfaI 568 GTAC GT^AC
BanII 890 GAGCTC G[GA]GC[CT]^C
BcnI 558 CCGGG CC^[GC]GG
BglII 853 AGATCT A^GATCT
BpmI 769 CTCCAG CTGGAG(16/14)
BsaI 658 GAGACC GGTCTC(1/5)
BsaWI 98 ACCGGA [AT]^CCGG[AT]
BsmFI 626 GGGAC GGGAC(10/14)
BsmI 88 GAATGC GAATGC(1/-1)
BspHI 251 TCATGA T^CATGA
BstAPI 943 GCAGTGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EaeI 555 TGGCCG [CT]^GGCC[GA]
EarI 805 GAAGAG CTCTTC(1/4)
EcoNI 369 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1057 GAATTC G^AATTC
HgaI 268 GACGC GACGC(5/10)
HindIII 967 AAGCTT A^AGCTT
Ksp632I 805 GAAGAG CTCTTC(1/4)
MaeII 111 ACGT A^CGT
NciI 558 CCGGG CC^[GC]GG
NdeI 983 CATATG CA^TATG
PflMI 672 CCAGTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 251 TCATGA T^CATGA
RsaI 568 GTAC GT^AC
SacI 890 GAGCTC GAGCT^C
SfaNI 562 GCATC GCATC(5/9)
SphI 78 GCATGC GCATG^C
SspI 213 AATATT AAT^ATT
Van91I 672 CCAGTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AciI 366 CCGC CCGC(-3/-1)
919 CCGC
AcsI 532 AAATTT [GA]^AATT[CT]
1057 GAATTC
ApoI 532 AAATTT [GA]^AATT[CT]
1057 GAATTC
AspHI 135 GAGCAC G[AT]GC[AT]^C
890 GAGCTC
BfaI 427 CTAG C^TAG
512 CTAG
BmyI 135 GAGCAC G[AGT]GC[ACT]^C
890 GAGCTC
BsiHKAI 135 GAGCAC G[AT]GC[AT]^C
890 GAGCTC
BsmAI 658 GAGAC GTCTC(1/5)
834 GTCTC
Bsp1286I 135 GAGCAC G[AGT]GC[ACT]^C
890 GAGCTC
DdeI 789 CTCAG C^T[ACGT]AG
1097 CTGAG
DsaI 83 CCATGG C^C[GA][CT]GG
778 CCATGG
EcoT14I 83 CCATGG C^C[AT][AT]GG
778 CCATGG
HapII 99 CCGG C^CGG
558 CCGG
HpaII 99 CCGG C^CGG
558 CCGG
HphI 42 GGTGA GGTGA(8/7)
96 TCACC
MaeI 427 CTAG C^TAG
512 CTAG
MslI 246 CACACTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
939 CATGGCAGTG
MspI 99 CCGG C^CGG
558 CCGG
NcoI 83 CCATGG C^CATGG
778 CCATGG
StyI 83 CCATGG C^C[AT][AT]GG
778 CCATGG
TaqI 585 TCGA T^CGA
1038 TCGA
Tsp45I 55 GTGAC ^GT[GC]AC
342 GTCAC
TthHB8I 585 TCGA T^CGA
1038 TCGA
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AluI AlwI AvaII BsaJI BsiYI
BslI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DpnII EcoRII
FokI HaeIII HinfI MaeIII MboI
MboII MflI MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV NspI
Sau3AI Sau96I ScrFI SfcI TfiI
Tru9I Tsp509I TspRI XhoII