Restriction map for
pFN21AE6210
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BamHI BbrPI
BbsI BcgI BciVI BcnI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII Bst1107I BstAPI
BstBI BstUI BstYI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco52I
EcoNI EcoRI EcoRV EcoT22I FseI
HincII HindII HindIII HinfI HpaI
KpnI Ksp632I KspI MaeI MfeI
MflI MluI MroI MseI MunI
MvnI NciI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PstI PvuI RcaI RsaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TfiI Tru9I Tth111I TthHB8I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 553 GACGTC GACGT^C
AcsI 151 GAATTT [GA]^AATT[CT]
AocI 600 CCTCAGG CC^T[ACGT]AGG
Aor51HI 302 AGCGCT AGC^GCT
ApoI 151 GAATTT [GA]^AATT[CT]
AviII 443 TGCGCA TGC^GCA
BalI 674 TGGCCA TGG^CCA
BbeI 127 GGCGCC GGCGC^C
BbvCI 542 CCTCAGC CCTCAGC(-5/-2)
BclI 425 TGATCA T^GATCA
BpmI 616 CTCCAG CTGGAG(16/14)
BsaBI 426 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 649 GAGACC GGTCTC(1/5)
BseRI 598 CTCCTC GAGGAG(10/8)
BsmAI 649 GAGAC GTCTC(1/5)
BsmFI 742 GTCCC GGGAC(10/14)
BspMI 527 ACCTGC ACCTGC(4/8)
BsrFI 259 GCCGGC [GA]^CCGG[CT]
BssSI 697 CTCGTG CACGAG(-5/-1)
BstEII 342 GGTGACC G^GT[ACGT]ACC
BstPI 342 GGTGACC G^GT[ACGT]ACC
BstXI 677 CCATCGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 600 CCTCAGG CC^T[ACGT]AGG
Cfr10I 259 GCCGGC [GA]^CCGG[CT]
DpnII 426 GATC ^GATC
DraII 581 GGGGCCT [GA]G^G[ACGT]CC[CT]
Eco47III 302 AGCGCT AGC^GCT
Eco81I 600 CCTCAGG CC^T[ACGT]AGG
EcoO109I 581 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 342 GGTGACC G^GT[ACGT]ACC
FbaI 425 TGATCA T^GATCA
FspI 443 TGCGCA TGC^GCA
HapII 260 CCGG C^CGG
HgaI 207 GCGTC GACGC(5/10)
HpaII 260 CCGG C^CGG
KasI 127 GGCGCC G^GCGCC
MamI 426 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 426 GATC ^GATC
MluNI 674 TGGCCA TGG^CCA
MscI 674 TGGCCA TGG^CCA
MspA1I 166 CAGCTG C[AC]G^C[GT]G
MspI 260 CCGG C^CGG
NaeI 259 GCCGGC GCC^GGC
NarI 127 GGCGCC GG^CGCC
NcoI 644 CCATGG C^CATGG
NdeI 728 CATATG CA^TATG
NdeII 426 GATC ^GATC
NgoMIV 259 GCCGGC G^CCGGC
PshAI 651 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 166 CAGCTG CAG^CTG
SacI 613 GAGCTC GAGCT^C
Sau3AI 426 GATC ^GATC
SfoI 127 GGCGCC GGC^GCC
Tsp509I 152 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AvaI 132 CTCGGG C^[CT]CG[GA]G
362 CTCGGG
BbvI 314 GCAGC GCAGC(8/12)
441 GCTGC
BglI 73 GCCACCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
683 GCCTGGTGGGC
BsoBI 132 CTCGGG C^[CT]CG[GA]G
362 CTCGGG
DsaI 337 CCACGG C^C[GA][CT]GG
644 CCATGG
EcoT14I 77 CCAAGG C^C[AT][AT]GG
644 CCATGG
HaeII 127 GGCGCC [GA]GCGC^[CT]
302 AGCGCT
MaeII 329 ACGT A^CGT
554 ACGT
MaeIII 343 GTGAC ^GT[ACGT]AC
460 GTCAC
MboII 186 GAAGA GAAGA(8/7)
413 TCTTC
StyI 77 CCAAGG C^C[AT][AT]GG
644 CCATGG
TseI 314 GCAGC G^C[AT]GC
441 GCTGC
Tsp45I 343 GTGAC ^GT[GC]AC
460 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AcyI AluI AspHI AvaII
BanI BanII BmyI BsaHI BsaJI
BsiHKAI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI EaeI EcoRII FauI
Fnu4HI FokI HaeIII HhaI Hin1I
HinP1I HphI ItaI MnlI MslI
MvaI MwoI NlaIII NlaIV Sau96I
ScrFI SfaNI TspRI XcmI