Restriction map for
pFN21AE1700
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# NO CUT ENZYMES ( 160 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaBI BsaHI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeIII MamI MluI MroI
MslI MspA1I MvnI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI StuI StyI SwaI Tsp45I
Tth111I Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AciI 191 CCGC CCGC(-3/-1)
AcsI 552 GAATTC [GA]^AATT[CT]
AflIII 215 ACACGT A^C[GA][CT]GT
AlwNI 202 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 652 GGGCCC GGGCC^C
ApoI 552 GAATTC [GA]^AATT[CT]
AvaI 62 CCCGGG C^[CT]CG[GA]G
AvaII 293 GGTCC G^G[AT]CC
BalI 267 TGGCCA TGG^CCA
BamHI 439 GGATCC G^GATCC
BanI 275 GGTGCC G^G[CT][GA]CC
BanII 652 GGGCCC G[GA]GC[CT]^C
BfaI 103 CTAG C^TAG
BpmI 401 CTCCAG CTGGAG(16/14)
BsaAI 216 CACGTA [CT]AC^GT[GA]
BsaI 662 GAGACC GGTCTC(1/5)
BsaWI 158 TCCGGT [AT]^CCGG[AT]
BseRI 318 CTCCTC GAGGAG(10/8)
BsoBI 62 CCCGGG C^[CT]CG[GA]G
DraII 651 AGGGCCC [GA]G^G[ACGT]CC[CT]
DrdI 510 GACAAAGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 267 TGGCCA [CT]^GGCC[GA]
EcoO109I 651 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRI 552 GAATTC G^AATTC
HgaI 75 GACGC GACGC(5/10)
HphI 559 TCACC GGTGA(8/7)
MaeI 103 CTAG C^TAG
MaeII 217 ACGT A^CGT
MboII 369 GAAGA GAAGA(8/7)
MfeI 456 CAATTG C^AATTG
MluNI 267 TGGCCA TGG^CCA
MscI 267 TGGCCA TGG^CCA
MseI 496 TTAA T^TAA
MunI 456 CAATTG C^AATTG
NdeI 480 CATATG CA^TATG
NspI 466 GCATGC [GA]CATG^[CT]
SexAI 350 ACCTGGT A^CC[AT]GGT
SfaNI 306 GATGC GCATC(5/9)
SmaI 62 CCCGGG CCC^GGG
SmlI 116 CTCAAG C^T[CT][GA]AG
SphI 466 GCATGC GCATG^C
TaqI 660 TCGA T^CGA
Tru9I 496 TTAA T^TAA
TthHB8I 660 TCGA T^CGA
XcmI 537 CCACCTAAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 62 CCCGGG C^CCGGG
XmaI 62 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AfaI 219 GTAC GT^AC
579 GTAC
AluI 101 AGCT AG^CT
502 AGCT
AspHI 226 GAGCAC G[AT]GC[AT]^C
285 GTGCTC
BbvI 357 GCAGC GCAGC(8/12)
699 GCTGC
BcnI 62 CCCGG CC^[GC]GG
63 CCGGG
BsgI 199 GTGCAG GTGCAG(16/14)
355 GTGCAG
BsiHKAI 226 GAGCAC G[AT]GC[AT]^C
285 GTGCTC
BsiYI 240 CCAGGGTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
562 CCTGGAATTGG
BslI 240 CCAGGGTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
562 CCTGGAATTGG
BsmAI 662 GAGAC GTCTC(1/5)
399 GTCTC
BsmFI 294 GTCCC GGGAC(10/14)
429 GTCCC
BstF5I 236 CATCC GGATG(2/0)
87 GGATG
BstYI 439 GGATCC [GA]^GATC[CT]
669 AGATCC
Cac8I 173 GCCAGC GC[ACGT]^[ACGT]GC
466 GCATGC
DpnII 440 GATC ^GATC
670 GATC
Fnu4HI 357 GCAGC GC^[ACGT]GC
699 GCTGC
FokI 236 CATCC GGATG(9/13)
87 GGATG
HapII 63 CCGG C^CGG
159 CCGG
HinfI 204 GATTC G^A[ACGT]TC
339 GATTC
HpaII 63 CCGG C^CGG
159 CCGG
ItaI 357 GCAGC GC^[ACGT]GC
699 GCTGC
MboI 440 GATC ^GATC
670 GATC
MflI 439 GGATCC [GA]^GATC[CT]
669 AGATCC
MspI 63 CCGG C^CGG
159 CCGG
NciI 62 CCCGG CC^[GC]GG
63 CCGGG
NdeII 440 GATC ^GATC
670 GATC
RsaI 219 GTAC GT^AC
579 GTAC
Sau3AI 440 GATC ^GATC
670 GATC
TfiI 204 GATTC G^A[AT]TC
339 GATTC
TseI 357 GCAGC G^C[AT]GC
699 GCTGC
TspRI 229 CACTG CA[GC]TG[ACGT][ACGT]^
674 CACTG
XhoII 439 GGATCC [GA]^GATC[CT]
669 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AlwI BmyI BsaJI Bsp1286I BsrI
BssKI BstNI Cfr13I DdeI EcoRII
HaeIII MnlI MvaI MwoI NlaIII
NlaIV Sau96I ScrFI Tsp509I