Restriction map for
pFN21AE1503
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# NO CUT ENZYMES ( 138 enzymes) #
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Acc65I AccI AccIII AcsI AflII
AflIII AgeI Alw44I AlwNI Aor51HI
ApaI ApaLI ApoI AseI AsnI
Asp700I Asp718I AspI AvaII AviII
AvrII BanII BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BsaWI BseAI BsiEI BsiWI BsmFI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrFI BsrGI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EagI EarI EclXI
Eco47III Eco52I EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI HincII HindII HindIII HpaI
HphI KpnI Ksp632I MaeI MaeIII
MfeI MluI MroI MslI MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmeI PpuMI PshAI PshBI
PstI PvuI PvuII RcaI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfcI SfiI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tsp45I TspRI Tth111I TthHB8I Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 731 GACGTC GACGT^C
AclI 289 AACGTT AA^CGTT
AhdI 726 GACTGGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 322 CCTGAGG CC^T[ACGT]AGG
AscI 163 GGCGCGCC GG^CGCGCC
AspEI 726 GACTGGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 301 GTGCTC G[AT]GC[AT]^C
BamHI 192 GGATCC G^GATCC
BbeI 31 GGCGCC GGCGC^C
BbrPI 55 CACGTG CAC^GTG
BbvCI 476 CCTCAGC CCTCAGC(-5/-2)
BlpI 103 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 103 GCTGAGC GC^T[ACGT]AGC
BsaAI 55 CACGTG [CT]AC^GT[GA]
BsaBI 193 GATCCGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 754 GTGCAG GTGCAG(16/14)
BsiHKAI 301 GTGCTC G[AT]GC[AT]^C
BsmBI 733 CGTCTC CGTCTC(1/5)
BsmI 242 GCATTC GAATGC(1/-1)
BsrBI 106 GAGCGG CCGCTC(-3/-3)
BssSI 590 CACGAG CACGAG(-5/-1)
BstYI 192 GGATCC [GA]^GATC[CT]
Bsu36I 322 CCTGAGG CC^T[ACGT]AGG
CelII 103 GCTGAGC GC^T[ACGT]AGC
Eam1105I 726 GACTGGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 322 CCTGAGG CC^T[ACGT]AGG
EcoT14I 176 CCAAGG C^C[AT][AT]GG
FauI 719 CCCGC CCCGC(4/6)
HinfI 612 GAATC G^A[ACGT]TC
KasI 31 GGCGCC G^GCGCC
KspI 159 CCGCGG CCGC^GG
MamI 193 GATCCGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 192 GGATCC [GA]^GATC[CT]
MseI 206 TTAA T^TAA
MspA1I 159 CCGCGG C[AC]G^C[GT]G
NarI 31 GGCGCC GG^CGCC
PmaCI 55 CACGTG CAC^GTG
PmlI 55 CACGTG CAC^GTG
Psp1406I 289 AACGTT AA^CGTT
SacII 159 CCGCGG CCGC^GG
SfoI 31 GGCGCC GGC^GCC
StyI 176 CCAAGG C^C[AT][AT]GG
TfiI 612 GAATC G^A[AT]TC
Tru9I 206 TTAA T^TAA
Tsp509I 522 AATT ^AATT
XhoII 192 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 29 enzymes) #
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AcyI 31 GGCGCC G[GA]^CG[CT]C
731 GACGTC
AvaI 153 CCCGGG C^[CT]CG[GA]G
380 CCCGGG
BalI 586 TGGCCA TGG^CCA
605 TGGCCA
BanI 31 GGCGCC G^G[CT][GA]CC
313 GGCACC
BbsI 432 GAAGAC GAAGAC(2/6)
184 GTCTTC
BmyI 301 GTGCTC G[AGT]GC[ACT]^C
312 GGGCAC
BpuAI 432 GAAGAC GAAGAC(2/6)
184 GTCTTC
BsaHI 31 GGCGCC G[GA]^CG[CT]C
731 GACGTC
BseRI 474 CTCCTC GAGGAG(10/8)
349 GAGGAG
BsoBI 153 CCCGGG C^[CT]CG[GA]G
380 CCCGGG
Bsp1286I 301 GTGCTC G[AGT]GC[ACT]^C
312 GGGCAC
BsrI 727 ACTGG ACTGG(1/-1)
255 CCAGT
BstNI 345 CCTGG CC^[AT]GG
358 CCTGG
DpnII 12 GATC ^GATC
193 GATC
EcoNI 171 CCTCGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
628 CCTTTTGGAGG
EcoRII 345 CCTGG ^CC[AT]GG
358 CCTGG
HaeII 31 GGCGCC [GA]GCGC^[CT]
73 GGCGCT
Hin1I 31 GGCGCC G[GA]^CG[CT]C
731 GACGTC
MboI 12 GATC ^GATC
193 GATC
MboII 432 GAAGA GAAGA(8/7)
185 TCTTC
MluNI 586 TGGCCA TGG^CCA
605 TGGCCA
MscI 586 TGGCCA TGG^CCA
605 TGGCCA
MvaI 345 CCTGG CC^[AT]GG
358 CCTGG
NdeII 12 GATC ^GATC
193 GATC
NlaIII 318 CATG CATG^
609 CATG
Sau3AI 12 GATC ^GATC
193 GATC
SmaI 153 CCCGGG CCC^GGG
380 CCCGGG
XmaCI 153 CCCGGG C^CCGGG
380 CCCGGG
XmaI 153 CCCGGG C^CCGGG
380 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
BbvI BcnI BpmI BsaI BsaJI
BsiYI BslI BsmAI BsrDI BssHII
BssKI BstF5I BstUI Cac8I CfoI
Cfr13I DdeI DsaI EaeI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII ItaI MaeII MnlI MspI
MvnI MwoI NciI NlaIV RsaI
Sau96I ScrFI SfaNI TseI XcmI