Restriction map for
pFN21AE3932
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# NO CUT ENZYMES ( 174 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AfaI AflII AgeI AhdI
Alw44I AlwI AlwNI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspHI AspI AviII
AvrII BalI BamHI BbrPI BbsI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmFI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstYI BstZ17I Cfr10I ClaI
CpoI DpnII DraI DraII DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HgaI HincII HindII
HindIII HpaI KpnI Ksp632I KspI
MaeI MamI MboI MboII MfeI
MflI MluI MluNI MroI MscI
MseI MunI MvnI NaeI NcoI
NdeI NdeII NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsaI RsrII SacI
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfcI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tsp509I Tth111I XbaI
XcmI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AcyI 152 GGCGCC G[GA]^CG[CT]C
AflIII 65 ACACGT A^C[GA][CT]GT
AocI 197 CCTCAGG CC^T[ACGT]AGG
Asp700I 51 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 338 CTCGAG C^[CT]CG[GA]G
BanI 152 GGCGCC G^G[CT][GA]CC
BanII 259 GAGCCC G[GA]GC[CT]^C
BbeI 152 GGCGCC GGCGC^C
BbvCI 129 CCTCAGC CCTCAGC(-5/-2)
BlpI 4 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4 GCTGAGC GC^T[ACGT]AGC
BsaHI 152 GGCGCC G[GA]^CG[CT]C
BsoBI 338 CTCGAG C^[CT]CG[GA]G
BsrI 382 ACTGG ACTGG(1/-1)
Bsu36I 197 CCTCAGG CC^T[ACGT]AGG
CelII 4 GCTGAGC GC^T[ACGT]AGC
DsaI 224 CCACGG C^C[GA][CT]GG
EaeI 500 TGGCCG [CT]^GGCC[GA]
Eco81I 197 CCTCAGG CC^T[ACGT]AGG
EcoT14I 357 CCTTGG C^C[AT][AT]GG
HaeII 152 GGCGCC [GA]GCGC^[CT]
Hin1I 152 GGCGCC G[GA]^CG[CT]C
HphI 242 TCACC GGTGA(8/7)
KasI 152 GGCGCC G^GCGCC
MaeII 67 ACGT A^CGT
MslI 231 CACTGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 289 CAGCTG C[AC]G^C[GT]G
NarI 152 GGCGCC GG^CGCC
PaeR7I 338 CTCGAG C^TCGAG
PflMI 539 CCATCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 289 CAGCTG CAG^CTG
SfaNI 482 GCATC GCATC(5/9)
SfoI 152 GGCGCC GGC^GCC
StyI 357 CCTTGG C^C[AT][AT]GG
TaqI 339 TCGA T^CGA
TthHB8I 339 TCGA T^CGA
Van91I 539 CCATCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 338 CTCGAG C^TCGAG
XmnI 51 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AccI 343 GTCTAC GT^[AC][GT]AC
417 GTCTAC
AluI 23 AGCT AG^CT
290 AGCT
BcnI 18 CCGGG CC^[GC]GG
149 CCCGG
BpmI 143 CTCCAG CTGGAG(16/14)
256 CTGGAG
BstXI 80 CCAAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
185 CCACAGTGCTGG
CfoI 153 GCGC GCG^C
330 GCGC
HapII 18 CCGG C^CGG
150 CCGG
HhaI 153 GCGC GCG^C
330 GCGC
HinP1I 153 GCGC G^CGC
330 GCGC
HinfI 141 GACTC G^A[ACGT]TC
341 GAGTC
HpaII 18 CCGG C^CGG
150 CCGG
MspI 18 CCGG C^CGG
150 CCGG
NciI 18 CCGGG CC^[GC]GG
149 CCCGG
NlaIII 237 CATG CATG^
268 CATG
PleI 141 GACTC GAGTC(4/5)
341 GAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AvaII BbvI BmyI BsaJI
BsiYI BslI Bsp1286I BssKI BstF5I
BstNI Cac8I Cfr13I DdeI EcoRII
Fnu4HI FokI HaeIII ItaI MaeIII
MnlI MvaI MwoI NlaIV Sau96I
ScrFI SmlI TseI Tsp45I TspRI