Restriction map for
pFN21AE3934
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI AocI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstZ17I
Bsu36I CelII ClaI CpoI DsaI
EagI Eam1105I EclXI Eco52I Eco81I
EcoT22I FbaI FseI FspI HgaI
Hin1I HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MunI MvnI NarI NcoI NdeI
NotI NruI NsiI NspI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Alw44I 878 GTGCAC G^TGCAC
ApaI 365 GGGCCC GGGCC^C
ApaLI 878 GTGCAC G^TGCAC
BalI 1469 TGGCCA TGG^CCA
BamHI 777 GGATCC G^GATCC
BglI 490 GCCACCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1600 TACGTG [CT]AC^GT[GA]
BseRI 1435 GAGGAG GAGGAG(10/8)
BspMI 171 ACCTGC ACCTGC(4/8)
BsrBI 424 GAGCGG CCGCTC(-3/-3)
BsrFI 1283 GCCGGC [GA]^CCGG[CT]
BstEII 990 GGTGACC G^GT[ACGT]ACC
BstPI 990 GGTGACC G^GT[ACGT]ACC
BstYI 777 GGATCC [GA]^GATC[CT]
Cfr10I 1283 GCCGGC [GA]^CCGG[CT]
DraI 391 TTTAAA TTT^AAA
DraII 1145 GGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 790 GACTCCCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 699 CCTGTCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1145 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 990 GGTGACC G^GT[ACGT]ACC
EcoRI 847 GAATTC G^AATTC
EcoRV 481 GATATC GAT^ATC
HincII 1084 GTTGAC GT[CT]^[GA]AC
HindII 1084 GTTGAC GT[CT]^[GA]AC
MflI 777 GGATCC [GA]^GATC[CT]
MluNI 1469 TGGCCA TGG^CCA
MscI 1469 TGGCCA TGG^CCA
MseI 392 TTAA T^TAA
NaeI 1283 GCCGGC GCC^GGC
NgoMIV 1283 GCCGGC G^CCGGC
NheI 1312 GCTAGC G^CTAGC
PaeR7I 40 CTCGAG C^TCGAG
PpuMI 1145 GGGACCC [GA]G^G[AT]CC[CT]
PstI 580 CTGCAG CTGCA^G
SacI 1365 GAGCTC GAGCT^C
SapI 1372 GAAGAGC GCTCTTC(1/4)
ScaI 311 AGTACT AGT^ACT
SfcI 580 CTGCAG C^T[GA][CT]AG
TfiI 225 GATTC G^A[AT]TC
Tru9I 392 TTAA T^TAA
XcmI 494 CCAAGGCTGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 40 CTCGAG C^TCGAG
XhoII 777 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 220 GTAC GT^AC
312 GTAC
AlwNI 373 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1238 CAGAAGCTG
Aor51HI 584 AGCGCT AGC^GCT
956 AGCGCT
BfaI 472 CTAG C^TAG
1313 CTAG
BsaI 168 GAGACC GGTCTC(1/5)
596 GAGACC
BsgI 811 CTGCAC GTGCAG(16/14)
1213 GTGCAG
BstXI 671 CCACCCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
737 CCAAGTGCTTGG
DraIII 51 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1462 CACTGTGTG
EarI 1372 GAAGAG CTCTTC(1/4)
1387 GAAGAG
Eco47III 584 AGCGCT AGC^GCT
956 AGCGCT
Ksp632I 1372 GAAGAG CTCTTC(1/4)
1387 GAAGAG
MaeI 472 CTAG C^TAG
1313 CTAG
MslI 105 CACCGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
753 CATGAAGGTG
PflMI 648 CCAAGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
953 CCAAGCGCTGG
PvuII 376 CAGCTG CAG^CTG
397 CAGCTG
RsaI 220 GTAC GT^AC
312 GTAC
SmlI 40 CTCGAG C^T[CT][GA]AG
298 CTTGAG
Van91I 648 CCAAGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
953 CCAAGCGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BcnI BmyI BpmI
BpuAI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII EaeI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PshAI Sau3AI Sau96I ScrFI
SfaNI SmaI StyI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I XmaCI
XmaI