Restriction map for
pFN21AE2350
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI AocI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrGI Bst1107I BstAPI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DdeI DraI
DraIII Eam1105I Eco47III Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FspI HgaI HincII HindII HindIII
HpaI KpnI MaeI MamI MfeI
MluI MroI MseI MunI NdeI
NheI NotI NruI NsiI NspI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SalI SbfI ScaI
SexAI SfiI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tsp509I
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 666 GTCTAC GT^[AC][GT]AC
AcyI 564 GGCGCC G[GA]^CG[CT]C
AfaI 902 GTAC GT^AC
Alw44I 844 GTGCAC G^TGCAC
ApaI 1321 GGGCCC GGGCC^C
ApaLI 844 GTGCAC G^TGCAC
BamHI 169 GGATCC G^GATCC
BbeI 564 GGCGCC GGCGC^C
BglI 58 GCCCTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 564 GGCGCC G[GA]^CG[CT]C
BsgI 323 GTGCAG GTGCAG(16/14)
BsiEI 117 CGGCCG CG[GA][CT]^CG
BsmAI 889 GTCTC GTCTC(1/5)
BspHI 608 TCATGA T^CATGA
BsrFI 119 GCCGGC [GA]^CCGG[CT]
BssHII 96 GCGCGC G^CGCGC
BssSI 735 CTCGTG CACGAG(-5/-1)
BstBI 817 TTCGAA TT^CGAA
BstYI 169 GGATCC [GA]^GATC[CT]
Cfr10I 119 GCCGGC [GA]^CCGG[CT]
DrdI 625 GACACCACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 117 CGGCCG C^GGCCG
EclXI 117 CGGCCG C^GGCCG
Eco52I 117 CGGCCG C^GGCCG
FseI 118 GGCCGGCC GGCCGG^CC
HaeII 564 GGCGCC [GA]GCGC^[CT]
Hin1I 564 GGCGCC G[GA]^CG[CT]C
KasI 564 GGCGCC G^GCGCC
KspI 836 CCGCGG CCGC^GG
MaeII 398 ACGT A^CGT
MflI 169 GGATCC [GA]^GATC[CT]
NaeI 119 GCCGGC GCC^GGC
NarI 564 GGCGCC GG^CGCC
NgoMIV 119 GCCGGC G^CCGGC
NspV 817 TTCGAA TT^CGAA
PpuMI 947 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 1291 CAGCTG CAG^CTG
RcaI 608 TCATGA T^CATGA
RsaI 902 GTAC GT^AC
SacII 836 CCGCGG CCGC^GG
SapI 258 GAAGAGC GCTCTTC(1/4)
SfoI 564 GGCGCC GGC^GCC
SfuI 817 TTCGAA TT^CGAA
TfiI 198 GATTC G^A[AT]TC
XhoII 169 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AlwI 170 GATCC GGATC(4/5)
169 GGATC
AlwNI 1276 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1288 CAGCAGCTG
BanI 564 GGCGCC G^G[CT][GA]CC
991 GGTGCC
BbsI 963 GAAGAC GAAGAC(2/6)
487 GTCTTC
BpuAI 963 GAAGAC GAAGAC(2/6)
487 GTCTTC
BsrBI 114 GAGCGG CCGCTC(-3/-3)
1105 GAGCGG
BsrDI 585 CATTGC GCAATG(2/0)
299 GCAATG
DpnII 170 GATC ^GATC
331 GATC
DraII 947 AGGACCC [GA]G^G[ACGT]CC[CT]
1320 AGGGCCC
EarI 258 GAAGAG CTCTTC(1/4)
979 GAAGAG
EcoO109I 947 AGGACCC [GA]G^G[ACGT]CC[CT]
1320 AGGGCCC
HphI 230 TCACC GGTGA(8/7)
746 TCACC
Ksp632I 258 GAAGAG CTCTTC(1/4)
979 GAAGAG
MaeIII 623 GTGAC ^GT[ACGT]AC
1309 GTGAC
MboI 170 GATC ^GATC
331 GATC
MslI 417 CACCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
921 CACAGATGTG
NcoI 1113 CCATGG C^CATGG
1220 CCATGG
NdeII 170 GATC ^GATC
331 GATC
PflMI 798 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1029 CCAGGTCTTGG
PleI 868 GACTC GAGTC(4/5)
1311 GACTC
SacI 4 GAGCTC GAGCT^C
22 GAGCTC
Sau3AI 170 GATC ^GATC
331 GATC
SfaNI 1233 GATGC GCATC(5/9)
344 GCATC
TaqI 575 TCGA T^CGA
818 TCGA
Tsp45I 623 GTGAC ^GT[GC]AC
1309 GTGAC
TthHB8I 575 TCGA T^CGA
818 TCGA
Van91I 798 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1029 CCAGGTCTTGG
XcmI 132 CCAAAAAGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
395 CCAACGTGGAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AluI AspHI AvaII
BalI BanII BbvI BcnI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DsaI EaeI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MboII MluNI MnlI MscI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIII NlaIV PstI Sau96I ScrFI
SfcI StyI TseI TspRI