Restriction map for
pFN21AE3594
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccI AccIII AclI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BamHI BanII BbrPI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmAI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstEII
BstPI BstYI BstZ17I CelII Cfr10I
Cfr13I ClaI CpoI DpnII DraI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRV EcoT14I EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI MaeI MaeII MaeIII
MamI MboI MfeI MflI MluI
MluNI MroI MscI MseI MunI
NaeI NciI NcoI NdeI NdeII
NgoMIV NheI NotI NruI NsiI
NspI PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsaI RsrII SacI SalI
SapI Sau3AI Sau96I SbfI ScaI
SexAI SfiI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
StyI SwaI Tru9I Tsp45I Tth111I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AcsI 439 GAATTC [GA]^AATT[CT]
ApoI 439 GAATTC [GA]^AATT[CT]
Asp700I 417 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 113 GTGCTC G[AT]GC[AT]^C
BanI 100 GGCGCC G^G[CT][GA]CC
BbeI 100 GGCGCC GGCGC^C
BbsI 327 GAAGAC GAAGAC(2/6)
BmyI 113 GTGCTC G[AGT]GC[ACT]^C
BpmI 232 CTGGAG CTGGAG(16/14)
BpuAI 327 GAAGAC GAAGAC(2/6)
BseRI 235 GAGGAG GAGGAG(10/8)
BsiHKAI 113 GTGCTC G[AT]GC[AT]^C
BsiYI 490 CCACGGAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 490 CCACGGAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 260 GAATGC GAATGC(1/-1)
Bsp1286I 113 GTGCTC G[AGT]GC[ACT]^C
BspMI 398 ACCTGC ACCTGC(4/8)
BsrBI 61 CCGCTC CCGCTC(-3/-3)
BsrI 231 ACTGG ACTGG(1/-1)
BstBI 324 TTCGAA TT^CGAA
BstXI 224 CCAATGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI 47 TGGCCG [CT]^GGCC[GA]
EarI 417 GAAGAG CTCTTC(1/4)
EcoRI 439 GAATTC G^AATTC
HaeII 100 GGCGCC [GA]GCGC^[CT]
HapII 38 CCGG C^CGG
HgaI 55 GCGTC GACGC(5/10)
HpaII 38 CCGG C^CGG
HphI 396 TCACC GGTGA(8/7)
KasI 100 GGCGCC G^GCGCC
Ksp632I 417 GAAGAG CTCTTC(1/4)
KspI 50 CCGCGG CCGC^GG
MslI 397 CACCTGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 50 CCGCGG C[AC]G^C[GT]G
MspI 38 CCGG C^CGG
NarI 100 GGCGCC GG^CGCC
NlaIII 403 CATG CATG^
NlaIV 100 GGCGCC GG[ACGT]^[ACGT]CC
NspV 324 TTCGAA TT^CGAA
PflMI 490 CCACGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 382 CTGCAG CTGCA^G
SacII 50 CCGCGG CCGC^GG
SfoI 100 GGCGCC GGC^GCC
SfuI 324 TTCGAA TT^CGAA
SmlI 128 CTCAAG C^T[CT][GA]AG
SspI 120 AATATT AAT^ATT
Tsp509I 440 AATT ^AATT
TspRI 230 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 490 CCACGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 417 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 29 enzymes) #
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AccII 51 CGCG CG^CG
96 CGCG
AcyI 54 GGCGTC G[GA]^CG[CT]C
100 GGCGCC
AocI 214 CCTCAGG CC^T[ACGT]AGG
274 CCTCAGG
BsaHI 54 GGCGTC G[GA]^CG[CT]C
100 GGCGCC
BsmFI 57 GTCCC GGGAC(10/14)
449 GTCCC
BssKI 26 CCAGG ^CC[ACGT]GG
89 CCTGG
BstAPI 220 GCATCCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
372 GCATCCGTTGC
BstF5I 221 CATCC GGATG(2/0)
373 CATCC
BstNI 26 CCAGG CC^[AT]GG
89 CCTGG
BstUI 51 CGCG CG^CG
96 CGCG
Bsu36I 214 CCTCAGG CC^T[ACGT]AGG
274 CCTCAGG
CfoI 95 GCGC GCG^C
101 GCGC
DsaI 50 CCGCGG C^C[GA][CT]GG
490 CCACGG
Eco81I 214 CCTCAGG CC^T[ACGT]AGG
274 CCTCAGG
EcoRII 26 CCAGG ^CC[AT]GG
89 CCTGG
FokI 221 CATCC GGATG(9/13)
373 CATCC
HaeIII 48 GGCC GG^CC
488 GGCC
HhaI 95 GCGC GCG^C
101 GCGC
Hin1I 54 GGCGTC G[GA]^CG[CT]C
100 GGCGCC
HinP1I 95 GCGC G^CGC
101 GCGC
HinfI 205 GATTC G^A[ACGT]TC
422 GATTC
MvaI 26 CCAGG CC^[AT]GG
89 CCTGG
MvnI 51 CGCG CG^CG
96 CGCG
ScrFI 26 CCAGG CC^[ACGT]GG
89 CCTGG
SfaNI 220 GCATC GCATC(5/9)
372 GCATC
SfcI 154 CTACAG C^T[GA][CT]AG
382 CTGCAG
TaqI 325 TCGA T^CGA
468 TCGA
TfiI 205 GATTC G^A[AT]TC
422 GATTC
TthHB8I 325 TCGA T^CGA
468 TCGA
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# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
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AciI AluI BbvI BsaJI Cac8I
DdeI FauI Fnu4HI ItaI MboII
MnlI MwoI TseI