Restriction map for
pFN21AE5511
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BbrPI
BcgI BciVI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsgI
BsiEI BsiWI BsmI BspDI BspLU11I
BsrBI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco52I EcoO65I EcoRV EcoT22I
FseI FspI HincII HindII HindIII
HpaI KpnI MaeI MfeI MluI
MseI MunI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SalI SapI
SbfI ScaI SfuI SmlI SnaBI
SpeI SphI Sse8387I SspI SwaI
TaqI TfiI Tru9I TthHB8I Van91I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcsI 1156 GAATTC [GA]^AATT[CT]
AocI 870 CCTGAGG CC^T[ACGT]AGG
Aor51HI 152 AGCGCT AGC^GCT
ApoI 1156 GAATTC [GA]^AATT[CT]
AspHI 82 GTGCTC G[AT]GC[AT]^C
AvaII 723 GGACC G^G[AT]CC
BamHI 866 GGATCC G^GATCC
BbvCI 635 CCTCAGC CCTCAGC(-5/-2)
BclI 446 TGATCA T^GATCA
BsaBI 447 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1207 GAGACC GGTCTC(1/5)
BsiHKAI 82 GTGCTC G[AT]GC[AT]^C
BsmBI 498 GAGACG CGTCTC(1/5)
Bsp1407I 644 TGTACA T^GTACA
BspMI 89 ACCTGC ACCTGC(4/8)
BsrDI 900 GCAATG GCAATG(2/0)
BsrGI 644 TGTACA T^GTACA
BssHII 52 GCGCGC G^CGCGC
BssSI 133 CACGAG CACGAG(-5/-1)
BstXI 1133 CCAGCACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 866 GGATCC [GA]^GATC[CT]
Bsu36I 870 CCTGAGG CC^T[ACGT]AGG
DsaI 183 CCGCGG C^C[GA][CT]GG
EarI 430 CTCTTC CTCTTC(1/4)
Eco47III 152 AGCGCT AGC^GCT
Eco81I 870 CCTGAGG CC^T[ACGT]AGG
EcoRI 1156 GAATTC G^AATTC
EcoT14I 759 CCAAGG C^C[AT][AT]GG
FbaI 446 TGATCA T^GATCA
HgaI 953 GCGTC GACGC(5/10)
Ksp632I 430 CTCTTC CTCTTC(1/4)
KspI 183 CCGCGG CCGC^GG
MaeIII 1004 GTGAC ^GT[ACGT]AC
MamI 447 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 866 GGATCC [GA]^GATC[CT]
PpuMI 722 GGGACCT [GA]G^G[AT]CC[CT]
PstI 1087 CTGCAG CTGCA^G
PvuII 18 CAGCTG CAG^CTG
SacII 183 CCGCGG CCGC^GG
SexAI 725 ACCTGGT A^CC[AT]GGT
SfcI 1087 CTGCAG C^T[GA][CT]AG
SgrAI 291 CACCGGCG C[GA]^CCGG[CT]G
SspBI 644 TGTACA T^GTACA
StyI 759 CCAAGG C^C[AT][AT]GG
Tsp45I 1004 GTGAC ^GT[GC]AC
Tsp509I 1157 AATT ^AATT
XhoII 866 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccIII 494 TCCGGA T^CCGGA
956 TCCGGA
AfaI 582 GTAC GT^AC
645 GTAC
ApaI 24 GGGCCC GGGCC^C
506 GGGCCC
AspI 265 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
724 GACCTGGTC
BanII 24 GGGCCC G[GA]GC[CT]^C
506 GGGCCC
BbsI 109 GTCTTC GAAGAC(2/6)
787 GTCTTC
BpuAI 109 GTCTTC GAAGAC(2/6)
787 GTCTTC
BsaWI 494 TCCGGA [AT]^CCGG[AT]
956 TCCGGA
BseAI 494 TCCGGA T^CCGGA
956 TCCGGA
BseRI 160 GAGGAG GAGGAG(10/8)
235 GAGGAG
BspEI 494 TCCGGA T^CCGGA
956 TCCGGA
BspHI 467 TCATGA T^CATGA
1187 TCATGA
EcoNI 36 CCTGAACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
510 CCTGCCCCAGG
HinfI 676 GACTC G^A[ACGT]TC
914 GACTC
MroI 494 TCCGGA T^CCGGA
956 TCCGGA
MslI 255 CATCCGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
792 CATCCTCATG
PflFI 265 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
724 GACCTGGTC
PleI 676 GACTC GAGTC(4/5)
914 GACTC
RcaI 467 TCATGA T^CATGA
1187 TCATGA
RsaI 582 GTAC GT^AC
645 GTAC
SfiI 240 GGCCACTCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1071 GGCCTCGTTGGCC
SmaI 27 CCCGGG CCC^GGG
1145 CCCGGG
StuI 762 AGGCCT AGG^CCT
1070 AGGCCT
Tth111I 265 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
724 GACCTGGTC
XmaCI 27 CCCGGG C^CCGGG
1145 CCCGGG
XmaI 27 CCCGGG C^CCGGG
1145 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AvaI BalI BanI BbeI BbvI
BcnI BglI BmyI BpmI BsaHI
BsaJI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HpaII HphI ItaI
KasI MaeII MboI MboII MluNI
MnlI MscI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI SfoI TseI
TspRI