Restriction map for
pFN21AE1325
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI AocI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbsI BbvCI BcgI BciVI BclI
BfrI BlnI BpuAI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MamI MfeI MluI MroI MseI
MunI MvnI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Tsp45I Tth111I XbaI
XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 316 GTCTAC GT^[AC][GT]AC
AcsI 1322 GAATTC [GA]^AATT[CT]
Alw44I 716 GTGCAC G^TGCAC
AlwI 1305 GGATC GGATC(4/5)
ApaLI 716 GTGCAC G^TGCAC
ApoI 1322 GAATTC [GA]^AATT[CT]
BalI 137 TGGCCA TGG^CCA
BbeI 923 GGCGCC GGCGC^C
BfaI 659 CTAG C^TAG
BglII 795 AGATCT A^GATCT
BsgI 421 GTGCAG GTGCAG(16/14)
BsrBI 574 CCGCTC CCGCTC(-3/-3)
BstAPI 1413 GCACGTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 795 AGATCT [GA]^GATC[CT]
DraIII 719 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 636 CCGTGG C^C[GA][CT]GG
EaeI 137 TGGCCA [CT]^GGCC[GA]
EcoRI 1322 GAATTC G^AATTC
FauI 370 GCGGG CCCGC(4/6)
HphI 842 TCACC GGTGA(8/7)
KasI 923 GGCGCC G^GCGCC
MaeI 659 CTAG C^TAG
MaeIII 1001 GTAAC ^GT[ACGT]AC
MflI 795 AGATCT [GA]^GATC[CT]
MluNI 137 TGGCCA TGG^CCA
MscI 137 TGGCCA TGG^CCA
MslI 538 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 923 GGCGCC GG^CGCC
NspI 735 GCATGT [GA]CATG^[CT]
PaeR7I 959 CTCGAG C^TCGAG
SfoI 923 GGCGCC GGC^GCC
SmlI 959 CTCGAG C^T[CT][GA]AG
StuI 404 AGGCCT AGG^CCT
Tsp509I 1323 AATT ^AATT
XcmI 342 CCACACCTTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 959 CTCGAG C^TCGAG
XhoII 795 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Aor51HI 1296 AGCGCT AGC^GCT
1362 AGCGCT
BanI 923 GGCGCC G^G[CT][GA]CC
1257 GGTGCC
BbrPI 745 CACGTG CAC^GTG
1414 CACGTG
BlpI 181 GCTGAGC GC^T[ACGT]AGC
1358 GCTCAGC
Bpu1102I 181 GCTGAGC GC^T[ACGT]AGC
1358 GCTCAGC
BsaAI 745 CACGTG [CT]AC^GT[GA]
1414 CACGTG
BsaI 548 GAGACC GGTCTC(1/5)
853 GAGACC
CelII 181 GCTGAGC GC^T[ACGT]AGC
1358 GCTCAGC
Eco47III 1296 AGCGCT AGC^GCT
1362 AGCGCT
EcoNI 75 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
417 CCTCGTGCAGG
EcoT14I 194 CCAAGG C^C[AT][AT]GG
552 CCAAGG
NlaIII 736 CATG CATG^
1176 CATG
PleI 101 GACTC GAGTC(4/5)
804 GAGTC
PmaCI 745 CACGTG CAC^GTG
1414 CACGTG
PmlI 745 CACGTG CAC^GTG
1414 CACGTG
PpuMI 755 GGGACCC [GA]G^G[AT]CC[CT]
1071 GGGACCC
PstI 143 CTGCAG CTGCA^G
1366 CTGCAG
SacI 1356 GAGCTC GAGCT^C
1383 GAGCTC
SfcI 143 CTGCAG C^T[GA][CT]AG
1366 CTGCAG
SfiI 405 GGCCTTCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1087 GGCCGAGGGGGCC
SmaI 28 CCCGGG CCC^GGG
1067 CCCGGG
StyI 194 CCAAGG C^C[AT][AT]GG
552 CCAAGG
TaqI 788 TCGA T^CGA
960 TCGA
TthHB8I 788 TCGA T^CGA
960 TCGA
XmaCI 28 CCCGGG C^CCGGG
1067 CCCGGG
XmaI 28 CCCGGG C^CCGGG
1067 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwNI
ApaI AspHI AvaI AvaII BanII
BbvI BcnI BglI BmyI BpmI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BssSI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
ItaI Ksp632I MaeII MboI MboII
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIV PflMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
TfiI TseI TspRI Van91I